Light-sheet microscopy articles from across Nature Portfolio

Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation compared to conventional light microscopy methods.

Latest Research and Reviews

Research
27 February 2023 | Open Access

Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture
- L. Dvinskikh
- , H. Sparks
- & C. Dunsby
Scientific Reports 13, 3342
Research
14 January 2023 | Open Access

An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish

Studying chemosensory processing desires precise chemical cue presentation, behavioral response monitoring, and large-scale neuronal activity recording. Here, the authors report a fluidics-based toolkit for studying chemosensation in larval zebrafish, and used it to reveal the brainwide neural representations of cadaverine sensing and its binasal input-dependent behavioral avoidance.
- Samuel K. H. Sy
- , Danny C. W. Chan
- & Ho Ko
Nature Communications 14, 227
Research
28 November 2022 | Open Access

A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems
- Paulina M. Wysmolek
- , Filippo D. Kiessler
- & Friedhelm Serwane
Scientific Reports 12, 20420
Research
02 November 2022 | Open Access

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

We present experimentally unsupervised learned deconvolution for light-sheet microscopy using propagation-invariant beams. By training a generative adversarial network using simulated paired images consistent with the physics of the imaging system, and real unpaired experimental data, we create a network that can rapidly and robustly deconvolve microscopy images encoded by a complex point-spread function, such as from Airy or Bessel beams. The method is facile and does not need data for training outwith that obtained during conventional imaging.
- Philip Wijesinghe
- , Stella Corsetti
- & Kishan Dholakia
Light: Science & Applications 11, 319
Research | 24 October 2022

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

The longstanding goal of combining the optical sectioning of light-sheet illumination and the resolving power of multidirectional structured illumination microscopy is realized using an oblique plane microscope for improved fast 3D subcellular imaging.
- Bingying Chen
- , Bo-Jui Chang
- & Reto P. Fiolka
Nature Methods 19, 1419-1426
Reviews | 20 October 2022

Practical considerations for quantitative light sheet fluorescence microscopy

This Perspective describes common pitfalls that can occur when using light sheet microscopy and offers guidance for improved quantitative imaging with these instruments.
- Chad M. Hobson
- , Min Guo
- & Teng-Leong Chew
Nature Methods 19, 1538-1549

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News and Comment

News & Views | 24 October 2022

Doubling the resolution of light-sheet fluorescence microscopy

A combination of light-sheet fluorescence microscopy (LSFM) with structured illumination doubles resolving power over LSFM alone. We show a practical implementation using a single objective for illumination and fluorescence detection and demonstrate its use for rapid volumetric imaging.

Nature Methods 19, 1355-1356
News & Views | 10 October 2022

Combining a deep neural network with physical properties for super-resolution live imaging

We integrated the pre-characterized physical model of super-resolution (SR) microscopy into a deep neural network to guide the denoising of raw images for high-quality SR image reconstruction. This approach enabled us to investigate a wide variety of fragile and rapidly evolving bioprocesses at ultrahigh spatiotemporal resolution over extended imaging times.

Nature Biotechnology 41, 328-329
News & Views | 11 May 2022

Imaging cleared tissues made easy

A flexible open-top light-sheet microscope has been developed that can perform deep three-dimensional imaging on all clearing protocols with low and high optical resolution.
- Shigeaki Kanatani
- & Per Uhlén
Nature Methods 19, 527-529
News & Views | 21 March 2022

DaXi: pushing the limits of single-objective light-sheet microscopy

A new single-objective light-sheet microscope has been developed that uses novel optics and imaging protocols to increase resolution without compromising imaging speed and volume.

Nature Methods 19, 414-415
News | 28 June 2021

Reto Fiolka

A flight path into biology and fast 3D projection of sample dynamics.
- Vivien Marx
Nature Methods 18, 697
Comments & Opinion | 06 January 2021

Single-objective light sheet microscopy

Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.
- Rita Strack
Nature Methods 18, 28

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Light-sheet microscopy articles from across Nature Portfolio

Latest Research and Reviews

Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture

An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish

A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems

Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams

Resolution doubling in light-sheet microscopy via oblique plane structured illumination

Practical considerations for quantitative light sheet fluorescence microscopy

News and Comment

Doubling the resolution of light-sheet fluorescence microscopy

Combining a deep neural network with physical properties for super-resolution live imaging

Imaging cleared tissues made easy

DaXi: pushing the limits of single-objective light-sheet microscopy

Reto Fiolka

Single-objective light sheet microscopy

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