Featured
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Brief Communication
| Open Access
Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems
This work presents a highly versatile open-top, dual-view and dual-illumination light-sheet microscope for live imaging of large specimens.
- Franziska Moos
- , Simon Suppinger
- & Prisca Liberali
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Article
| Open Access
Super-sectioning with multi-sheet reversible saturable optical fluorescence transitions (RESOLFT) microscopy
Multi-sheet RESOLFT combines the speed and optical sectioning of light-sheet fluorescence microscopy with reversibly photoswitchable fluorescent proteins to enable fast, volumetric super-resolution imaging in live cells.
- Andreas Bodén
- , Dirk Ollech
- & Ilaria Testa
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Article
| Open Access
Image restoration of degraded time-lapse microscopy data mediated by near-infrared imaging
InfraRed-mediated Image Restoration (IR2) uses deep learning to combine the benefits of deep-tissue imaging with NIR probes and the convenience of imaging with GFP for improved time-lapse imaging of embryogenesis.
- Nicola Gritti
- , Rory M. Power
- & Jan Huisken
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Research Highlight |
Capturing hyperspectral images
A single-shot hyperspectral phasor camera (SHy-Cam) enables fast, multiplexed volumetric imaging.
- Rita Strack
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Research Briefing |
Doubling the resolution of light-sheet fluorescence microscopy
A combination of light-sheet fluorescence microscopy (LSFM) with structured illumination doubles resolving power over LSFM alone. We show a practical implementation using a single objective for illumination and fluorescence detection and demonstrate its use for rapid volumetric imaging.
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Article |
Resolution doubling in light-sheet microscopy via oblique plane structured illumination
The longstanding goal of combining the optical sectioning of light-sheet illumination and the resolving power of multidirectional structured illumination microscopy is realized using an oblique plane microscope for improved fast 3D subcellular imaging.
- Bingying Chen
- , Bo-Jui Chang
- & Reto P. Fiolka
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Perspective |
Practical considerations for quantitative light sheet fluorescence microscopy
This Perspective describes common pitfalls that can occur when using light sheet microscopy and offers guidance for improved quantitative imaging with these instruments.
- Chad M. Hobson
- , Min Guo
- & Teng-Leong Chew
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News & Views |
Imaging cleared tissues made easy
A flexible open-top light-sheet microscope has been developed that can perform deep three-dimensional imaging on all clearing protocols with low and high optical resolution.
- Shigeaki Kanatani
- & Per Uhlén
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Article |
A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues
A ‘hybrid’ open-top light-sheet microscope is described, which can be used for broad multi-scale volumetric imaging of one or more large tissues, cleared with diverse protocols, and conveniently mounted on an array of sample holders.
- Adam K. Glaser
- , Kevin W. Bishop
- & Jonathan T. C. Liu
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Research Briefing |
DaXi: pushing the limits of single-objective light-sheet microscopy
A new single-objective light-sheet microscope has been developed that uses novel optics and imaging protocols to increase resolution without compromising imaging speed and volume.
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Article
| Open Access
DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
The DaXi single-objective light-sheet microscope achieves fast, high-quality imaging of large volumes. DaXi’s design allows increased scanning range without sacrificing imaging speed or quality, multiview imaging and versatile sample mounting.
- Bin Yang
- , Merlin Lange
- & Loic A. Royer
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Article |
Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales
Combining a double-ring modulated SPIM with reduced side lobes and a sectionalized deep-learning based super-resolution algorithm enables fast, high-resolution, volumetric imaging of organelle interactions and dynamics in live cells.
- Yuxuan Zhao
- , Meng Zhang
- & Yu-Hui Zhang
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Article |
Universal autofocus for quantitative volumetric microscopy of whole mouse brains
RAPID (rapid autofocusing via pupil-split image phase detection) is a sample-agnostic real-time autofocus method for widefield microscopy. RAPID removes most image degradation in large, cleared samples for enhanced quantitative analyses.
- L. Silvestri
- , M. C. Müllenbroich
- & F. S. Pavone
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This Month |
Reto Fiolka
A flight path into biology and fast 3D projection of sample dynamics.
- Vivien Marx
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Article |
Real-time multi-angle projection imaging of biological dynamics
Multiangle projection imaging accelerates volumetric imaging by up to two orders of magnitude and is readily implemented on diverse microscopes, including spinning disk confocal and light-sheet microscopes.
- Bo-Jui Chang
- , James D. Manton
- & Reto Fiolka
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Method to Watch |
Single-objective light sheet microscopy
Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.
- Rita Strack
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Research Highlight |
Be still, my beating heart
An optical gating strategy allows day-long light-sheet imaging of the developing heart in zebrafish embryos.
- Rita Strack
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Article |
Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0
SCAPE 2.0 is a versatile imaging platform that enables real-time three-dimensional microscopy of cellular function and dynamic motion in living organisms at over 100 volumes per second with minimal photodamage, and high-throughput structural imaging in fixed, cleared and expanded samples.
- Venkatakaushik Voleti
- , Kripa B. Patel
- & Elizabeth M. C. Hillman
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Brief Communication |
The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue
The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.
- Fabian F. Voigt
- , Daniel Kirschenbaum
- & Fritjof Helmchen
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Article |
Robust and automated detection of subcellular morphological motifs in 3D microscopy images
A computational workflow combining image segmentation, computer graphics and supervised machine learning enables automated and robust 3D analysis of the coupling of cell shape and signaling.
- Meghan K. Driscoll
- , Erik S. Welf
- & Gaudenz Danuser
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Brief Communication |
Oblique-plane single-molecule localization microscopy for tissues and small intact animals
Single-molecule oblique-plane microscopy (obSTORM) enables deep volumetric super-resolution imaging in a light-sheet microscopy platform that is convenient for standard tissues and small intact animals.
- Jeongmin Kim
- , Michal Wojcik
- & Xiang Zhang
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This Month |
Stephan Preibisch
Staying mobile and building a Toy-Story-related way to stitch terabyte-sized images in microscopy.
- Vivien Marx
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Brief Communication |
BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples
BigStitcher enables fast and accurate alignment and reconstruction of terabyte-sized imaging datasets of cleared and expanded samples.
- David Hörl
- , Fabio Rojas Rusak
- & Stephan Preibisch
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Article |
Light-sheet microscopy in the near-infrared II window
Light-sheet microscopy in the NIR-II window enables rapid volumetric imaging of tissues at impressive depths in vivo without invasive preparations owing to the reduced light scattering and tissue autofluorescence at these wavelengths.
- Feifei Wang
- , Hao Wan
- & Hongjie Dai
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Brief Communication |
Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution
Epi-illumination SPIM enables fast, volumetric, high-resolution, subcellular imaging of any sample compatible with a standard inverted fluorescence microscope.
- Bin Yang
- , Xingye Chen
- & Bo Huang
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Brief Communication |
Instantaneous isotropic volumetric imaging of fast biological processes
Iso-LFM enables rapid, instantaneous volumetric imaging of biological processes with isotropic and improved resolution by simultaneously capturing orthogonal light fields.
- Nils Wagner
- , Nils Norlin
- & Robert Prevedel
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Research Highlight |
Mapping mouse development
Adaptive light-sheet microscopy enables imaging of mouse development through early organogenesis with single-cell resolution.
- Rita Strack
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Article |
An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas
An ex vivo 3D culture model of mycobacterial granulomas recapitulates the in vivo physiology of these structures and enables longitudinal imaging studies. The platform allows genetic and pharmacological manipulation of this key structure.
- Mark R. Cronan
- , Molly A. Matty
- & David M. Tobin
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Article |
Brain-wide circuit interrogation at the cellular level guided by online analysis of neuronal function
Imaging of neuronal activity across the whole zebrafish brain in combination with online analysis allows for manipulating neuronal activity according to function. This approach is used to ablate or activate neurons in fictively swimming zebrafish larvae.
- Nikita Vladimirov
- , Chen Wang
- & Misha B. Ahrens
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Research Highlight |
Corrected in vivo imaging
Aberration correction adds depth to light-sheet microscopy.
- Zachary J. Lapin
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Research Highlight |
Simulating the imaging process in scattering tissue
Biobeam is a software package that simulates optical effects during imaging within scattering tissue, which could help improve image acquisition and extraction.
- Nina Vogt
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Methods in Brief |
Tilted light-sheet microscopy with 3D point-spread functions
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Research Highlights |
Reflecting on light sheets
Reflective coverslips can improve spatiotemporal resolution and collection efficiency in diSPIM light-sheet fluorescence microscopy.
- Christian Schnell
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Methods in Brief |
Imaging fast subcellular dynamics with light sheets
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Review Article |
A guide to light-sheet fluorescence microscopy for multiscale imaging
This Review introduces the fundamental considerations for building a light sheet microscope, describes the pros and cons associated with available implementations, and offers practical advice for users.
- Rory M Power
- & Jan Huisken
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Technology Feature |
Microscopy: OpenSPIM 2.0
A maturing open hardware and open-source software movement seeks to expand DIY light-sheet microscopy.
- Vivien Marx
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Article |
Shrinkage-mediated imaging of entire organs and organisms using uDISCO
uDISCO clearing renders whole animals transparent and capitalizes on shrinkage to image them. This method allows the analysis of intact nervous systems and whole-body screens for transplanted cells and human tissue samples after prolonged storage.
- Chenchen Pan
- , Ruiyao Cai
- & Ali Ertürk
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Research Highlights |
Tunable light-sheet microscopy
Tiling light-sheet microscopy improves live cell imaging of multicellular organisms.
- Rita Strack
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Technology Feature |
Optimizing probes to image cleared tissue
Probes and imaging finesse for a clearer view of tissue.
- Vivien Marx
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Brief Communication |
Inverted light-sheet microscope for imaging mouse pre-implantation development
An inverted light-sheet microscope enables imaging of mouse embryos from zygote to blastocyst with minimal photodamage and high resolution for automatic lineage tree reconstruction, allowing new insight into cell fate specification.
- Petr Strnad
- , Stefan Gunther
- & Jan Ellenberg
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Research Highlights |
Super-resolution in live cells
Two strategies improve structured illumination microscopy for use in live-cell super-resolution imaging.
- Rita Strack
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Article |
Whole-animal functional and developmental imaging with isotropic spatial resolution
IsoView microscopy achieves rapid isotropic-resolution imaging of large, nontransparent samples using simultaneous light-sheet illumination and fluorescence detection in four orthogonal directions.
- Raghav K Chhetri
- , Fernando Amat
- & Philipp J Keller
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Methods in Brief |
Axially swept light-sheet microscopy
Selective-plane-activation structured illumination microscopy