Lab-on-a-chip articles from across Nature Portfolio

Flow cytometry is often hindered by undesired fluctuations in fluorescence intensity. Here, the authors propose high-throughput fluorescence lifetime imaging flow cytometry, which enables imaging at a rate of over 10,000 cells per second and, therefore, enhances the capabilities of cellular analysis.

Physical characterisation of proteins is challenging. Here the authors report single-molecule microfluidic diffusional sizing (smMDS) to enable calibration-free single-molecule diffusional-sizing based monitoring of protein hydrodynamic radii even within heterogenous multicomponent mixtures.

Kambez Benam reviews preclinical approaches to assess lung health impacts of e-cigarettes, highlighting limitations of current strategies in capturing 3D lung architecture and inhalation mechanics. The review article emphasizes the promise of Organs-on-Chips and Bioinspired Robotics.

This Perspective discusses the need for standards that allow for comparisons of quantitative physiological features in microphysiological systems and humans.

Sushila Maharjan is a biochemist and bioengineer and co-founder of Nepal’s Research Institute for Bioscience and Biotechnology (RIBB).

Lab-grown ‘mini-colons’ enable the simulation of colorectal tumor dynamics and the high-resolution study of interactions with cells from the tumor’s native microenvironment. These next-generation organoids offer a wealth of new experimental opportunities, propelling the frontiers of cancer modeling in multiple directions.

Pharmaceutical companies continue to advocate for the use of in vitro models towards the reduction of animal use in drug discovery and development while acknowledging that further advancements are needed to heighten the models’ current state of readiness.

The physical phenotypes of malignant cells in tissue biopsies can be rapidly characterized at high throughput via deformability cytometry after singularizing the cells into a suspension by using a tissue grinder.

A combined modality enables real-time imaging of mouse lungs and spans whole-organ to cellular scales.

We developed a 3D human neuroimmune axis model to study the interplay of brain innate immune cells and peripheral adaptive immune cells in Alzheimer’s disease. Alzheimer’s disease pathology induced a marked increase in CD8⁺ T cell infiltration, exacerbating neurodegeneration. The CXCL10–CXCR3 pathway has a key role in mediating this process.