Lab-on-a-chip

  • Article
    | Open Access

    The ever-growing need for highly functional, compact, and integrated microfluidic devices often incurs lengthy and expensive manufacturing processes. Here, authors introduce a generalized 3D printing process that enables fast parallel fabrication of miniaturized, high resolution 3D components.

    • Jose L. Sanchez Noriega
    • , Nicholas A. Chartrand
    •  & Gregory P. Nordin
  • Article
    | Open Access

    Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIFTER) and use this to probe the effects of cellular stress.

    • Julea Vlassakis
    • , Louise L. Hansen
    •  & Amy E. Herr
  • Article
    | Open Access

    Placenta accreta spectrum (PAS) is a high-risk obstetrical complication associated with significant morbidity and mortality. Here the authors discover a uniquely high prevalence of circulating trophoblasts clusters in PAS and explore their diagnostic potential to augment current diagnostic modalities for the early detection of PAS.

    • Yalda Afshar
    • , Jiantong Dong
    •  & Yazhen Zhu
  • Article
    | Open Access

    Sustainable strategies for shepherding active particles are at the heart of many prospective applications. Here, Palacios et al. use the emerging topological properties of a microfluidic maze array to passively guide self-propelled colloids from the interior to the edges of the device.

    • Lucas S. Palacios
    • , Serguei Tchoumakov
    •  & Adolfo G. Grushin
  • Article
    | Open Access

    Here, the authors present a pseudo-diamagnetophoresis mattertronic approach for programmable manipulation of label-free cells. Immersed in biocompatible ferrofluids, single cells are moved along linear negative micromagnetic patterns, switched at eclipse diode patterns and stored in potential wells.

    • Sandhya Rani Goudu
    • , Hyeonseol Kim
    •  & CheolGi Kim
  • Article
    | Open Access

    Devices for droplet generation are at the heart of many microfluidic applications but difficult to tailor for specific cases. Lashkaripour et al. show how design customization can greatly be simplified by combining rapid prototyping with data-driven machine learning strategies.

    • Ali Lashkaripour
    • , Christopher Rodriguez
    •  & Douglas Densmore
  • Article
    | Open Access

    Multi-Omic approaches are a powerful way for obtaining in-depth understanding of a cell’s state. Here the authors present DISCO, combining digital microfluidics, laser cell lysis, and artificial intelligence-driven image processing to analyze single-cell genomes, transcriptomes and proteomes in a mixed population.

    • Julian Lamanna
    • , Erica Y. Scott
    •  & Aaron R. Wheeler
  • Article
    | Open Access

    On-chip synthesis and screening has been used to automate drug discovery but on-chip analysis still remains a major limitation. Here, the authors report on a dendrimer-based surface patterning method to create nanodroplet arrays on materials which allow for on-chip high-throughput analysis.

    • Maximilian Benz
    • , Arndt Asperger
    •  & Pavel A. Levkin
  • Article
    | Open Access

    Current methods of neuronal network imaging cannot be used for continuous, long-term functional recordings. Here, the authors present a dual-mode high-density microelectrode array, which can simultaneously record in full-frame and high-signal-to-noise modes for label-free electrophysiological measurements.

    • Xinyue Yuan
    • , Manuel Schröter
    •  & Urs Frey
  • Article
    | Open Access

    Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no need for labeling.

    • Nao Nitta
    • , Takanori Iino
    •  & Keisuke Goda
  • Article
    | Open Access

    Calorimetrically measuring the heat of single cells is currently not possible due to the sensitivity of existing calorimeters. Here the authors present on-chip single cell calorimetry, with a sensitivity over ten-fold greater than the current gold-standard.

    • Sahngki Hong
    • , Edward Dechaumphai
    •  & Renkun Chen
  • Article
    | Open Access

    High-throughput rheological measurements of cells and cell clusters by microfluidics is limited by fixed channel dimensions. Here the authors create virtual fluidic channels inside the cuvette of commercial flow cytometers to dynamically tune channel cross section to enable rheological measurements from cells and cell clusters.

    • Muzaffar H. Panhwar
    • , Fabian Czerwinski
    •  & Oliver Otto
  • Article
    | Open Access

    There is a need for more robust sample delivery methods for serial crystallography. Here the authors present the design and characterization of ultracompact 3D microfluidic devices that can be printed, which require less sample, have a lower background signal and allow 3D mixing for time resolved experiments.

    • Juraj Knoška
    • , Luigi Adriano
    •  & Michael Heymann
  • Article
    | Open Access

    Developing an in vitro blood-brain-barrier (BBB) model that reproduces the organ’s complex structure and function is an open challenge. Here the authors present a BBB-on-a-chip that includes endothelial cells, pericytes and a 3D astrocytic network which resembles the morphology and function of astrocytes in the BBB in vivo.

    • Song Ih Ahn
    • , Yoshitaka J. Sei
    •  & YongTae Kim
  • Article
    | Open Access

    Large scale culture of adherent cells would benefit from a platform for continuous monitoring and control of cell growth and culture conditions. Here the authors develop an integrated, smart cell culture platform where cells are grown on multiple layers of thin sensors that can be wirelessly integrated across several incubators.

    • Kyoung Won Cho
    • , Seok Joo Kim
    •  & Dae-Hyeong Kim
  • Article
    | Open Access

    Cellular systems have numerous mechanisms to control gene expression. Here the authors build a Tet-On system with conditional destablising elements to regulate gene expression and protein stability, allowing fine modulation of mESC signalling pathways.

    • Elisa Pedone
    • , Lorena Postiglione
    •  & Lucia Marucci
  • Article
    | Open Access

    The glomerular filtration barrier is a complex structure in charge of renal ultrafiltration. Here the authors present a glomerulus-on-a-chip for disease modelling and high-throughput drug screening where human podocytes and human glomerular endothelial cells are separated by an extracellular matrix resembling the in vivo basement membrane.

    • Astgik Petrosyan
    • , Paolo Cravedi
    •  & Stefano Da Sacco
  • Article
    | Open Access

    Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimulation, and dPLA in a smaller reaction volume.

    • Jing Lin
    • , Christian Jordi
    •  & Savaş Tay
  • Article
    | Open Access

    The understanding of liquid-liquid phase separation is crucial to cell biology and benefits from cell-mimicking in vitro assays. Here, the authors develop a microfluidic platform to study coacervate formation inside liposomes and show the potential of these hybrid systems to create synthetic cells.

    • Siddharth Deshpande
    • , Frank Brandenburg
    •  & Cees Dekker
  • Article
    | Open Access

    DNA hydrogels have received considerable attention in analytical science but limitations still exist in the applications of intelligent hydrogels. Here, the authors describe a DNA hydrogel sensor for quantitative detection of cocaine based on the permeability change in a DNA hydrogel film.

    • Yansheng Li
    • , Yanli Ma
    •  & Yongqiang Wen
  • Article
    | Open Access

    Myocardial tissue undergoes steady functional decline when cultured in vitro. Here, the authors report a protocol for culture of human cardiac slices that allows maintenance of contractility for up to four months, and show that the model is suitable for evaluation of drug safety, as exemplified for drugs interfering with cardiomyocyte repolarization.

    • Carola Fischer
    • , Hendrik Milting
    •  & Andreas Dendorfer
  • Article
    | Open Access

    Single-cell detection methods are limited by the trade-off between flow rate and measurement precision. Here the authors introduce active loading, an optically triggered microfluidic system to concentrate diluted cell samples, which reduces clogging and decreases processing time in single-cell assays.

    • Nicholas L. Calistri
    • , Robert J. Kimmerling
    •  & Scott R. Manalis
  • Article
    | Open Access

    Plasmacytoid dendritic cells (pDC) are a pivotal component of the immune system. Here, the authors utilize single-cell microfluidics to interrogate the human pDC compartment and reveal a subset of type I IFN secreting pDCs that is regulated by stochastic gene expression and amplified by microenvironmental cues.

    • Florian Wimmers
    • , Nikita Subedi
    •  & Jurjen Tel
  • Article
    | Open Access

    Contamination is an obstacle to the functioning of microfluidic devices. Here the authors exploit acoustic streaming to manipulate droplets which float on a layer of immiscible oil. This prevents contamination and enables rewritability by which different fluids can be used on the same substrate.

    • Steven Peiran Zhang
    • , James Lata
    •  & Tony Jun Huang
  • Article
    | Open Access

    Rhinovirus (RV) infections can trigger acute exacerbations of respiratory diseases. Here, Niespodziana et al. develop a PreDicta chip that identifies the culprit RV strain from small blood samples and show that RV-A and RV-C strains are associated with most severe symptoms.

    • Katarzyna Niespodziana
    • , Katarina Stenberg-Hammar
    •  & Rudolf Valenta
  • Article
    | Open Access

    A bottleneck in developing new anti-fibrosis therapies is the absence of suitable in vitro models that recapitulate key features of fibrogenesis. Here the authors develop a tissue-on-a-chip model of lung fibrosis and test the therapeutic efficacy of two recent FDA-approved drugs.

    • Mohammadnabi Asmani
    • , Sanjana Velumani
    •  & Ruogang Zhao
  • Article
    | Open Access

    Spatial localization of genetic information is important for tissue heterogeneity but difficult to capture with current analytical techniques. Here the authors present “Pixelated RT-LAMP”, an approach that uses parallel on-chip reactions to provide the distribution of target sequences directly from tissue.

    • A. Ganguli
    • , A. Ornob
    •  & R. Bashir
  • Article
    | Open Access

    Detecting metastatic cells in tumor/lymph node samples of breast cancer patients is extremely important for diagnosis. Here the authors develop a microelectronic biochip that detect the presence of invasive/metastatic cells in unprocessed biopsies and performs better than the current gold standards.

    • Mohammad Saeid Nikshoar
    • , Mohammad Ali Khayamian
    •  & Mohammad Abdolahad
  • Article
    | Open Access

    Implementing a nucleic acid preconcentration method can improve the sensitivity of microfluidic analysis systems. Here Friedrich et al. concentrate DNA by many orders of magnitude using pressure-driven flow, which could lead to a simple and practical microanalysis platform.

    • Sarah M. Friedrich
    • , Jeffrey M. Burke
    •  & Tza-Huei Wang
  • Article
    | Open Access

    The complexity of fabricating and operating microfluidic devices limits their use. Walsh et al. describe a method in which circuits are printed as quickly and simply as writing with a pen, and liquids in them are confined by fluid instead of solid walls.

    • Edmond J. Walsh
    • , Alexander Feuerborn
    •  & Peter R. Cook
  • Article
    | Open Access

    The fabrication of vascularized 3D tissues requires an understanding of how material properties govern endothelial cell invasion into the surrounding matrix. Here the authors integrate a non-swelling synthetic hydrogel with a microfluidic device to study chemokine gradient-driven angiogenic sprouting and find that matrix degradability modulates the collectivity of cell migration.

    • Britta Trappmann
    • , Brendon M. Baker
    •  & Christopher S. Chen
  • Article
    | Open Access

    Efforts to determine the effects of drugs on epithelial barriers could benefit from better in vitro models. Here the authors develop a microfluidic device supporting the growth and function of extracellular matrix-supported intestinal tubules, and evaluate the effect of staurosporine and acetylsalicylic acid on barrier integrity.

    • Sebastiaan J. Trietsch
    • , Elena Naumovska
    •  & Paul Vulto
  • Article
    | Open Access

    The female reproductive tract constitutes the ovary, fallopian tubes, uterus, and cervix, but it is challenging to engineer this systemin vitro. Here, the authors develop a microfluidic device (EVATAR) with reproductive tract and peripheral tissues to replicate hormone release of a 28-day menstrual cycle.

    • Shuo Xiao
    • , Jonathan R. Coppeta
    •  & Teresa K. Woodruff
  • Article
    | Open Access

    Experimenter scoring of cellular imaging data can be biased. This study describes an automated and unbiased multidimensional phenotyping method that relies on machine learning and complex feature computation of imaging data, and identifies weak alleles affecting synapse morphology in live C. elegans.

    • Adriana San-Miguel
    • , Peri T. Kurshan
    •  & Hang Lu
  • Article
    | Open Access

    Analysis of bacteria and their response to antibiotics in real time is challenging. Here the authors report a microcantilever based system that can detect and discriminate between bacteria species and, due to the ability to discriminate between alive and dead samples, measure response to antibiotics.

    • Hashem Etayash
    • , M. F. Khan
    •  & Thomas Thundat