Kinetics

Reaction kinetics describes rate dynamics in chemical and biological systems. It allows for the experimental determination of reaction rates, from which rate constants may be estimated, in order to better understand biological systems modelled by reaction–diffusion processes.

Latest Research and Reviews

  • Research
    | Open Access

    Feng et al address the technical challenge of monitoring the protein folding during membrane translocation. Using thioredoxin as a model and with electric potential driving its translocation through single a-hemolysin nanopore, they observe that after translocation, folding is slower than in bulk due to existence of kinetic traps.

    • Jianfei Feng
    • , Pablo Martin-Baniandres
    • , Michael J. Booth
    • , Gianluca Veggiani
    • , Mark Howarth
    • , Hagan Bayley
    •  & David Rodriguez-Larrea
  • Research
    | Open Access

    T4 Lysozyme (T4L) is a model protein whose structure is extensively studied. Here the authors combine single-molecule and ensemble FRET measurements, FRET-positioning and screening and EPR spectroscopy to study the structural dynamics of T4L and describe its conformational landscape during the catalytic cycle by an extended Michaelis–Menten mechanism and identify an excited conformational state of the enzyme.

    • Hugo Sanabria
    • , Dmitro Rodnin
    • , Katherina Hemmen
    • , Thomas-Otavio Peulen
    • , Suren Felekyan
    • , Mark R. Fleissner
    • , Mykola Dimura
    • , Felix Koberling
    • , Ralf Kühnemuth
    • , Wayne Hubbell
    • , Holger Gohlke
    •  & Claus A. M. Seidel
  • Research |

    Single-molecule visualization shows that condensin—a motor protein that extrudes DNA in one direction only—can encounter and pass a second condensin molecule to form a new type of DNA loop that gathers DNA from both sides.

    • Eugene Kim
    • , Jacob Kerssemakers
    • , Indra A. Shaltiel
    • , Christian H. Haering
    •  & Cees Dekker
    Nature 579, 438-442
  • Research
    | Open Access

    The important tuberculosis drug pyrazinamide (PZA) is converted to its active form pyrazinoic acid (POA) in Mycobacterium tuberculosis (Mtb). Here the authors identify the pantothenate biosynthesis pathway enzyme aspartate decarboxylase (PanD) as the target of PZA and determine the POA bound Mtb PanD crystal structure.

    • Qingan Sun
    • , Xiaojun Li
    • , Lisa M. Perez
    • , Wanliang Shi
    • , Ying Zhang
    •  & James C. Sacchettini
  • Research
    | Open Access

    The poly(ADP-ribose) polymerases play a key role in maintaining genomic integrity by detecting DNA damage and mediating repair. Here the authors characterize the kinetics of PARP1 binding to a variety of nucleosomes harbouring DNA double-strand breaks.

    • Deepti Sharma
    • , Louis De Falco
    • , Sivaraman Padavattan
    • , Chang Rao
    • , Susana Geifman-Shochat
    • , Chuan-Fa Liu
    •  & Curt A. Davey

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