Immune cell isolation articles from across Nature Portfolio

Antigen-specific B cells constitute a small proportion of the total B cell population, making identification for downstream analysis challenging. Here, this is achieved by pairing fluorescent antigen tetramer probes with a sensitive enrichment approach.

When and how microglia engulf synapses and myelin is still unclear. Here, the authors provide a suite of flow cytometry-based approaches to quantify engulfment, paving the way for high-throughput assessment of microglial function in health and disease.

Exosomal PD-L1 (exoPD-L1) is a biomarker predicting immunotherapeutic responses. Here the authors report NanoEPIC, a nanoscale cytometry platform that enables phenotypic sorting and exoPD-L1 profiling from blood plasma by using magnetic-activated ranking to differentiate exosomal subpopulations.

Time-Dependent Cell-State Selection combines live cell imaging and single-cell RNA sequencing to characterize and analyse ILC2s during their activation, revealing a set of genes that are transitionally upregulated (TIGs) in ILC2s during this phase.

On 15–16 June 2022, the National Institute of Allergy and Infectious Diseases hosted a virtual workshop on the topic of T cell technologies to discuss assays, novel technology development, bench and clinical application of those technologies, and challenges and innovations in the field.

In the manufacturing of cell-based therapeutics, isolating cells via DNA aptamers is an attractive alternative to magnetic-activated cell sorting.

A highIL2/IFNGmRNA ratio identifies rare tumour-specific T cells that can persist in humans following adoptive transfer.