Immune cell isolation articles from across Nature Portfolio

This protocol enables single-cell multi-omic analysis of the mouse brain immune compartment to explore immune cell heterogeneity and activation in the healthy and diseased brain, using a workflow based on HD flow cytometry, scRNA-seq and CITE-seq.

The authors provide an optimized step-by-step protocol that uses mass cytometry to characterize well-known and emerging human dendritic cell populations in human blood and tissues. The protocol may also be applicable to other rare cell populations.

Bulk tumor preservation and dissociation enable multiple analyses of the brain tumor immune microenvironment via immunofluorescent staining and cell sorting followed by transcriptomics, genomics or in vitro culture of specific cell populations.

The detection of antigen-specific CD4⁺ T cells is improved with engineered peptide:MHCII tetramers.