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| Open AccessMechanism of glycoform specificity and in vivo protection by an anti-afucosylated IgG nanobody
Afucosylated immunoglobulins have been shown to enhance certain viral infections. Here, the authors generate and structurally characterize a unique nanobody that inhibits these pathogenic glycoforms.
- Aaron Gupta
- , Kevin S. Kao
- & Jeffrey V. Ravetch
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| Open AccessA neutrophil mimicking metal-porphyrin-based nanodevice loaded with porcine pancreatic elastase for cancer therapy
Porcine neutrophil elastase (PPE) has been demonstrated to have a histone H1 isoform dependent anti-tumour effect. Here, the authors develop a biomimetic nanoplatform combining PPE and elevated histone H1 release induced by porphyrin nuclear localisation signal peptide and demonstrate its efficacy in preclinical cancer models.
- Tingting Cui
- , Yu Zhang
- & Xiaogang Qu
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Article
| Open AccessHIV-1 CD4-binding site germline antibody–Env structures inform vaccine design
VRC01-class bNAbs against the CD4 binding site of HIV-1 Env are targets of vaccine design. Here, the authors structurally characterized germline versions of the VRC01-class bNAb, BG24, bound to Env. They reveal mechanisms of germline binding, informing the design of VRC01-class targeting immunogens.
- Kim-Marie A. Dam
- , Christopher O. Barnes
- & Pamela J. Bjorkman
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| Open AccessEngineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action
HER2 acts an oncogenic driver in numerous cancers. Here, the authors design an anti-HER2 biparatopic and tetravalent IgG fusion with inhibitory effects in a xenograft model.
- Florian Kast
- , Martin Schwill
- & Andreas Plückthun
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| Open AccessHigh throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.
- Sarah A. Overall
- , Jugmohit S. Toor
- & Nikolaos G. Sgourakis
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Article
| Open AccessreChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4+ memory T cells
Co-localizing chromatin modifications and regulators can exert a combinatorial effect on chromatin structure and function. Here the authors describe reChIP-seq and normR to identify co-localizing proteins in an unbiased genome-wide manner.
- Sarah Kinkley
- , Johannes Helmuth
- & Ho-Ryun Chung