Imaging studies articles from across Nature Portfolio

Bioimaging with photocontrol and multiplexing capability is vital for studying cellular interactions and dynamics, but multiplexed stimulated Raman scattering (SRS) imaging with reversible photocontrol is elusive. Here, the authors report SRS microscopy with Carbow-switch enabling multiplexed SRS imaging and tracking in live cells with reversible photocontrol and high spatiotemporal selectivity.

Near-infrared small molecule dyes are attractive in biophotonics, but generally rely on expanded aromatic structures to achieve this redshift. Here the authors report the use of a ground state antiaromatic strategy for 14 aminofluorene dyes, and their use in imaging kidney injury.

Single-molecule fluorescent probes can be used for nanoreporting, localization, and now multiplexing, but understanding their stochastic fluctuations in emission intensity is crucial for accurate signal interpretation. Here, the authors elucidate the blinking dynamics of rhodamine, BODIPY, and antraquinone dyes, demonstrate that multiplexing performance improves with photophysical differences, and suggest guidelines for the selection and design of organic fluorophores for single-molecule multiplexing.

Improving signal to noise ratio of Raman spectra is vital for the application. Here, authors show a noise learning method that learns the noise feature of a spectrometer. This improves the signal to noise ratio and makes deep learning to be instrument dependent instead of sample dependent.

To determine the physiologically relevant oligomeric form of membrane proteins is extremely challenging. Now an elegant method of counting the oligomers in membrane proteins in near-native states is presented, using photobleaching and nanodiscs formed directly from cellular membranes.

Hyperspectral imaging can reveal important information without the need for staining. To extract information from this extensive data, however, new methods are needed that can interpret the spatial and spectral patterns present in the images.

Bio-othogonal chemistry lets us determine the fate of a lignin monomer using a radical tag and EPR spectroscopy.

Overcoming the challenges of plastic detection in plants has made it possible to transfer many of the lessons learned from plant–metal nanoparticle interactions to plastic nanoparticles.

Real-space imaging reveals how thin layers of ice grow. Although the basal structure of bulk ice is still observed in 2D ice, the edges exhibit different molecular arrangements.