Holoenzymes articles from across Nature Portfolio

Latest Research and Reviews

• 

  Structure of dynein–dynactin on microtubules shows tandem adaptor binding

  The structure of the complete dynein–dynactin complex and its interaction with microtubules and cargo adaptors are visualized using cryo-electron microscopy.

  • Sami Chaaban
  • Andrew P. Carter

  Research07 Sept 2022 Nature

  Volume: 610, P: 212-216
• 

  Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis

  Helicobacter pylori FabX, a dehydrogenase/isomerase flavoprotein, is required for unsaturated fatty acid synthesis. Here, the authors characterize FabX substrate recognition and catalytic mechanism, and reveal that it contains an atypical [4Fe-4S] cluster, which is essential and participates in the catalytic cycle.

  • Jiashen Zhou
  • Lin Zhang
  • Liang Zhang

  ResearchOpen Access26 Nov 2021 Nature Communications

  Volume: 12, P: 6932
• 

  Structural basis of ribosomal RNA transcription regulation

  Ribosomal RNA (rRNA) expression is regulated at the initiation stage of RNA synthesis. Here, the authors report cryo-EM structures of E. coli RNA polymerase and rRNA promoter complex with DksA/ppGpp on the way to open complex formation, identifying key steps in promoter recognition and opening.

  • Yeonoh Shin
  • M. Zuhaib Qayyum
  • Katsuhiko S. Murakami

  ResearchOpen Access22 Jan 2021 Nature Communications

  Volume: 12, P: 528
• 

  Enzymatic kinetic resolution of desmethylphosphinothricin indicates that phosphinic group is a bioisostere of carboxyl group

  Escherichia coli glutamate decarboxylase is highly selective for its natural substrate, L-glutamate, which it converts to γ-aminobutyric acid (GABA). Here this enzyme is shown to also decarboxylate a phosphinyl analogue of glutamate, suggesting that the phosphinic group can be considered a bioisostere of the carboxylic group.

  • Daniela De Biase
  • Francesca Cappadocio
  • Alex Khomutov

  ResearchOpen Access02 Sept 2020 Communications Chemistry

  Volume: 3, P: 121
• 

  Structural basis of RNA cap modification by SARS-CoV-2

  Specific non-structural proteins (nsp) of SARS coronaviruses are involved in methylation of virally encoded mRNAs to mimic cellular mRNAs for protection against host innate immune restriction. Here, the authors present a high resolution structure of SARS-CoV-2 nsp16/nsp10 ternary complex in the presence of cognate RNA substrate analogue and methyl donor, S-adenosyl methionine, revealing unique ligand-binding sites that may represent alternative targets for antiviral development.

  • Thiruselvam Viswanathan
  • Shailee Arya
  • Yogesh K. Gupta

  ResearchOpen Access24 Jul 2020 Nature Communications

  Volume: 11, P: 3718
• 

  Structures of fungal and plant acetohydroxyacid synthases

  Structures of the acetohydroxyacid synthase complexes of Saccharomyces cerevisiae and Arabidopsis thaliana provide insights into the biosynthesis of and feedback inhibition by branched-chain amino acids.

  • Thierry Lonhienne
  • Yu Shang Low
  • Luke W. Guddat

  Research08 Jul 2020 Nature

  Volume: 586, P: 317-321