Glycomics

  • Article
    | Open Access

    Glycomics can uncover important molecular changes but measured glycans are highly interconnected and incompatible with common statistical methods, introducing pitfalls during analysis. Here, the authors develop an approach to identify glycan dependencies across samples to facilitate comparative glycomics.

    • Bokan Bao
    • , Benjamin P. Kellman
    •  & Nathan E. Lewis
  • Article
    | Open Access

    The fate of ocean carbon is determined by the balance between primary productivity and heterotrophic breakdown of that photosynthate. Here the authors show that diatoms produce a polysaccharide that resists bacterial degradation, accumulates, aggregates and stores carbon during spring blooms.

    • Silvia Vidal-Melgosa
    • , Andreas Sichert
    •  & Jan-Hendrik Hehemann
  • Article
    | Open Access

    While mass spectrometry-based proteomics largely relies on digesting proteins into peptides, there is no equivalent strategy for polysaccharide analysis. Here, the authors develop a chemical approach to break down poly- into oligosaccharides and present a workflow to identify polysaccharides by oligosaccharide fingerprinting.

    • Matthew J. Amicucci
    • , Eshani Nandita
    •  & Carlito B. Lebrilla
  • Review Article
    | Open Access

    Glycomics is gaining momentum in basic, translational and clinical research. Here, the authors review current reporting standards and analysis tools for mass-spectrometry-based glycomics, and propose an e-infrastructure for standardized reporting and online deposition of glycomics data.

    • Miguel A. Rojas-Macias
    • , Julien Mariethoz
    •  & Niclas G. Karlsson
  • Article
    | Open Access

    In-depth characterization of complex glycomes is complicated by the immense structural diversity of glycans. Here, the authors present a mass spectrometry-based strategy for untargeted, sensitive glycan profiling and identify 167 N-glycan compositions in total human plasma.

    • Guinevere S. M. Lageveen-Kammeijer
    • , Noortje de Haan
    •  & Manfred Wuhrer
  • Article
    | Open Access

    The glycome of parasites can have immunomodulatory properties or help to avoid immune surveillance, but details are unknown. Here, Martini et al. characterize the N-glycome of the canine heartworm, reveal an unprecedented complexity, particularly in anionic N-glycans, and determine recognition by components of the immune system.

    • Francesca Martini
    • , Barbara Eckmair
    •  & Katharina Paschinger
  • Article
    | Open Access

    Post-translational modifications can affect antibody function in health and disease, but identification of all variants is difficult using existing technologies. Here the authors develop a microfluidic method to identify and quantify low-abundance IgG N-glycans and show some of these IgGs can be used as biomarkers for rheumatoid arthritis.

    • Jing-Rong Wang
    • , Wei-Na Gao
    •  & Zhi-Hong Jiang
  • Article
    | Open Access

    Protein glycosylation is a heterogeneous post-translational modification that generates greater proteomic diversity that is difficult to analyze. Here the authors describe pGlyco 2.0, a workflow for the precise one step identification of intact N-glycopeptides at the proteome scale.

    • Ming-Qi Liu
    • , Wen-Feng Zeng
    •  & Peng-Yuan Yang
  • Article
    | Open Access

    The analysis of site-specific glycosylation of HIV Envelope glycoprotein (Env) is challenging as it contains 25–30 glycosylation sites with multiple glycan forms at each site. Here the authors present a generally applicable mass spectrometry-based method for site-specific analysis of protein glycosylation that they apply to the analysis of the HIV-1 Env.

    • Liwei Cao
    • , Jolene K. Diedrich
    •  & James C. Paulson