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Fluorescent proteins are proteins that absorb light and re-emit it at a longer wavelength. They can be genetically encoded as fusions to other proteins to act as labels.
Aberrant stem cell-like activity and impaired differentiation are central to the development of colorectal cancer. Here, authors develop a dual endogenous reporter system to identify functional regulators of aberrant stem cell and differentiation programs, showing that SMARCB1 restricts differentiation, and nominating other regulators with therapeutic potential.
Deng et al. expand the toolbox of neurotransmitter sensors with high-sensitivity green and red genetically encoded serotonin sensors. These are suitable for in vivo applications, as demonstrated in a variety of applications in mice.
G protein responses mediated by GPCRs may differ depending on their environment. Here, using highly sensitive Gi/o sensors, the authors reveal the specific pharmacological and Gi/o protein responses of some native GPCRs in neurons, and the influence of G protein composition.
FiLa biosensors allow real-time subcellular analysis of lactate metabolism. This protocol describes their preparation and characterization, and their use in in vitro and in vivo assays, under various nutritional and pharmacological conditions.
The exceptionally photostable green fluorescent protein StayGold has been monomerized in different laboratories, which has generated three unique monomeric variants that will enable new imaging applications.
ABACUS2 FRET biosensors allow an unparalleled live view of the dynamics of the plant hormone abscisic acid in plants. Well-watered roots accumulate abscisic acid in growing cells when shoots become dehydrated — a response that is essential to maintain root growth in low-humidity conditions.
Three groundbreaking studies have created a new generation of genetically encoded voltage indicators, empowering us to tackle a host of questions on our path toward understanding the brain.
Jane Liao and Allie C. Obermeyer explore the discovery, modification and applications of green fluorescent protein, best known for its use as a tool to cast light on cellular processes.
We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.
