Fluorescent proteins articles from across Nature Portfolio

Fluorescent proteins are proteins that absorb light and re-emit it at a longer wavelength. They can be genetically encoded as fusions to other proteins to act as labels.

Latest Research and Reviews

Research
06 June 2023 | Open Access

NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems

NADP(H) is a crucial cofactor, acting as a reducing agent in numerous pathways in living organisms. Here the authors report a ratiometric biosensor named NERNST, which can be used to estimate the NADP(H) redox status in bacterial, plant and animal cells and organelles.
- Pamela E. Molinari
- , Adriana R. Krapp
- & Matias D. Zurbriggen
Nature Communications 14, 3277
Research
23 May 2023 | Open Access

Effects of 5G-modulated 3.5 GHz radiofrequency field exposures on HSF1, RAS, ERK, and PML activation in live fibroblasts and keratinocytes cells
- Alexandre Joushomme
- , Rosa Orlacchio
- & Yann Percherancier
Scientific Reports 13, 8305
Research
04 May 2023 | Open Access

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

iGluSnFR variants with improved signal-to-noise ratios and targeting to postsynaptic sites have been developed, enabling the analysis of glutamatergic neurotransmission in vivo as illustrated in the mouse visual and somatosensory cortex.
- Abhi Aggarwal
- , Rui Liu
- & Kaspar Podgorski
Nature Methods 20, 925-934
Research
02 May 2023 | Open Access

Specific, sensitive and quantitative protein detection by in-gel fluorescence

Recombinant proteins in complex solutions are typically detected with tag-specific antibodies in Western blots. Here, the author describes an antibody-free alternative in which tagged proteins are detected directly in polyacrylamide gels via fluorophore-labelling of the tagged protein using a ligase.
- Adrian C. D. Fuchs
Nature Communications 14, 2505
Research
20 April 2023 | Open Access

Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

The NEMO series of genetically encoded calcium indicators report calcium activity in neuronal and non-neuronal cells with high signal-to-baseline ratio, which is shown in neuronal culture, slice preparations, in vivo and in planta.
- Jia Li
- , Ziwei Shang
- & Youjun Wang
Nature Methods 20, 918-924
Research | 27 March 2023

High-speed low-light in vivo two-photon voltage imaging of large neuronal populations

A suite of tools including positive-going voltage indicators, a high-speed two-photon microscope, and denoising software enables prolonged imaging of electrical activity in neurons with limited toxicity.
- Jelena Platisa
- , Xin Ye
- & Jerry L. Chen
Nature Methods, 1-9

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News and Comment

Comments & Opinion | 30 March 2023

Giving the green light

Jane Liao and Allie C. Obermeyer explore the discovery, modification and applications of green fluorescent protein, best known for its use as a tool to cast light on cellular processes.
- Jane Liao
- & Allie C. Obermeyer
Nature Chemistry 15, 588
News & Views | 27 March 2023

A brilliant monomeric red fluorescent protein combining high brightness and fast maturation

We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.

Nature Methods 20, 497-498
Research Highlights | 10 February 2023

A cell’s growth rings

Two research groups independently develop tools to record the history of cellular dynamics.
- Hui Hua
Nature Methods 20, 174
Comments & Opinion | 12 January 2023

A light switch for targeted genomics

The combination of microscopy, targeted illumination and single-cell sequencing is driving applications from direct evolution to spatial omics.
- Rita Strack
Nature Methods 20, 32
News & Views | 07 November 2022

Robust fluorescent proteins for high-resolution microscopy and biochemical techniques

Hyperfolder yellow fluorescent protein (hfYFP) and its variants are fluorescent proteins with high chemical and thermal stability. They resist aggregation, withstand diverse chemical challenges and show promise in expansion and electron microscopies. The chloride resistance and uncanny stability in guanidinium of hfYFP enable fluorescence-guided protein purification under denaturing conditions.

Nature Methods 19, 1534-1535
Research Highlights | 10 June 2022

A fluorescent protein with staying power

A newly described fluorescent protein, StayGold, is bright and extremely photostable, enabling extended time-lapse imaging.
- Rita Strack
Nature Methods 19, 642

All News & Comment

Fluorescent proteins articles from across Nature Portfolio

Latest Research and Reviews

NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems

Effects of 5G-modulated 3.5 GHz radiofrequency field exposures on HSF1, RAS, ERK, and PML activation in live fibroblasts and keratinocytes cells

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Specific, sensitive and quantitative protein detection by in-gel fluorescence

Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

High-speed low-light in vivo two-photon voltage imaging of large neuronal populations

News and Comment

Giving the green light

A brilliant monomeric red fluorescent protein combining high brightness and fast maturation

A cell’s growth rings

A light switch for targeted genomics

Robust fluorescent proteins for high-resolution microscopy and biochemical techniques

A fluorescent protein with staying power

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