Fluorescent proteins articles from across Nature Portfolio

Fluorescent proteins are proteins that absorb light and re-emit it at a longer wavelength. They can be genetically encoded as fusions to other proteins to act as labels.

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News and Comment

  • Comments & Opinion |

    Jane Liao and Allie C. Obermeyer explore the discovery, modification and applications of green fluorescent protein, best known for its use as a tool to cast light on cellular processes.

    • Jane Liao
    •  & Allie C. Obermeyer
  • News & Views |

    We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.

    Nature Methods 20, 497-498
  • News & Views |

    Hyperfolder yellow fluorescent protein (hfYFP) and its variants are fluorescent proteins with high chemical and thermal stability. They resist aggregation, withstand diverse chemical challenges and show promise in expansion and electron microscopies. The chloride resistance and uncanny stability in guanidinium of hfYFP enable fluorescence-guided protein purification under denaturing conditions.

    Nature Methods 19, 1534-1535