Fluorescent probes

Fluorescent probes are molecules that absorb light of a specific wavelength and emit light of a different, typically longer, wavelength (a process known as fluorescence), and are used to study biological samples. The molecules, also known as fluorophores, can be attached to a target molecule and act as a marker for analysis with fluorescence microscopy.

Latest Research and Reviews

News and Comment

  • News & Views |

    Understanding the mechanics of acute kidney injury from toxins, ischemia and sepsis remains challenging. Molecular probes with high renal clearance have now been developed for real-time optical detection of early-stage biomarkers of drug-induced acute kidney injury, and for the understanding of the mechanisms of injury.

    • Stephen M. Hewitt
    •  & Robert A. Star
    Nature Materials 18, 1034-1035
  • News & Views |

    For decades chemists have focused on increasing the brightness of fluorophores. In super-resolution microscopy, however, fluorophores that preferentially exist in a non-fluorescent state, but occasionally re-arrange into a fluorescent form, can give better results.

    • Gražvydas Lukinavičius
    •  & Kai Johnsson
    Nature Chemistry 6, 663-664
  • News & Views |

    Tuning the luminescence lifetimes of upconversion nanocrystals through lanthanide doping provides new opportunities for optical multiplexing in the time domain for applications in imaging and security marking.

    • Renren Deng
    •  & Xiaogang Liu
    Nature Photonics 8, 10-12
  • News & Views |

    A new biocompatible near-infrared fluorescent probe enables super-resolution imaging of cellular proteins in live cells using a range of different labelling techniques.

    • Kathrin Lang
    •  & Jason W. Chin
    Nature Chemistry 5, 81-82
  • News & Views |

    Conjugation of a known, mechanism-based glycosidase inhibitor to sensitive fluorophores yielded unexpectedly potent and selective probes for quantifying active lysosomal glucocerebrosidase. These conjugates could prove to be invaluable tools for diagnosing and studying Gaucher disease.

    • Ethan D Goddard-Borger
    • , Tom Wennekes
    •  & Stephen G Withers