Fluorescence resonance energy transfer articles from across Nature Portfolio

By designing a fluorescent protein with a well characterized non-native ensemble, which captures the structural modulation of a substrate by the Hsp70/DnaJ/NEF system, Tiwari et al. resolved the individual steps of the disaggregation unfolding mechanism of Hsp70.

The ability to infer quantitative kinetic information from single-molecule FRET (smFRET) data can be challenging. Here the authors perform a blind benchmark study assessing different analysis tools used to infer kinetic rate constants from smFRET trajectories, testing on simulated and experimental data.

Afterglow luminescence is promising for non-background molecular imaging in vivo. Here the authors report a ratiometric afterglow luminescent nanoplatform to generate activatable afterglow probes for quantification of specific analytes including NO.

In this work, the authors demonstrate the application of multi-parameter photon-by-photon hidden Markov modeling (mpH²MM) on alternating laser excitation (ALEX)-based smFRET measurements. The utility of mpH²MM in identifying and quantifying dynamic biomolecular sub-populations is demonstrated in three different systems.

Fluorescent reporters spanning the visible spectrum are needed for imaging live cells and organisms. Here the authors report a collection of fluorogenic chromophores that cover the visible spectrum from blue to red using a single engineered and optimised protein tag.