Fluorescence imaging articles from across Nature Portfolio

A microscope objective inspired by the Schmidt telescope offers a large field of view, high numerical aperture, long working distance and compatibility with all homogeneous immersion media for versatile bioimaging.

We present the Schmidt objective, a novel astronomy-inspired concept for designing multi-immersion microscope objectives with high numerical aperture, long working distance and large field of view. The Schmidt objective uses a spherical mirror and a correction plate to focus light. It is well suited for high-resolution imaging deep inside cleared biological samples.

New three-photon miniature microscopes open the study of neuronal networks to those deep in the brains of behaving animals.

STARmap PLUS is a new spatial gene mapping method combined with histological staining. With STARmap PLUS, we created high-resolution, comprehensive maps of altered molecular pathways and reactive cells in a mouse model of Alzheimer’s disease. These maps enabled us to infer the trajectories of biological processes and cell states during disease progression.

A new twist on expansion microscopy called Magnify uses a mechanically sturdy gel to simultaneously anchor and expand diverse biological samples for super-resolution imaging.

Cellular senesence is believed to be a driver of aging. We designed and synthesized a photosensitive prodrug that destroys senescent cells by integrating multiple technologies that combine biomarker guidance with a fluorescence tag, target-site anchoring and photodynamic therapy, providing a strategy for monitoring and specifically eliminating senescent cells to regulate aging.