Fluorescence imaging

Fluorescence imaging is the visualization of fluorescent dyes or proteins as labels for molecular processes or structures. It enables a wide range of experimental observations including the location and dynamics of gene expression, protein expression and molecular interactions in cells and tissues.

Latest Research and Reviews

  • Protocols |

    This protocol describes the design and fabrication of nanoscale structures for studying intracellular responses to membrane curvature, which are visualized by fluorescence imaging; FIB–SEM is used to characterize the nanostructure–cell interface.

    • Xiao Li
    • , Laura Matino
    • , Wei Zhang
    • , Lasse Klausen
    • , Allister F. McGuire
    • , Claudia Lubrano
    • , Wenting Zhao
    • , Francesca Santoro
    •  & Bianxiao Cui
  • Research |

    SEDS family peptidoglycan transglycosylases, RodA and FtsW, in Staphylococcus aureus pair with the cognate transpeptidases PBP3 and PBP1 to mediate sidewall elongation and septal peptidoglycan incorporation, respectively, and maintain coccoid morphology.

    • Nathalie T. Reichmann
    • , Andreia C. Tavares
    • , Bruno M. Saraiva
    • , Ambre Jousselin
    • , Patricia Reed
    • , Ana R. Pereira
    • , João M. Monteiro
    • , Rita G. Sobral
    • , Michael S. VanNieuwenhze
    • , Fábio Fernandes
    •  & Mariana G. Pinho
  • Research |

    Light-sheet microscopy in the NIR-II window enables rapid volumetric imaging of tissues at impressive depths in vivo without invasive preparations owing to the reduced light scattering and tissue autofluorescence at these wavelengths.

    • Feifei Wang
    • , Hao Wan
    • , Zhuoran Ma
    • , Yeteng Zhong
    • , Qinchao Sun
    • , Ye Tian
    • , Liangqiong Qu
    • , Haotian Du
    • , Mingxi Zhang
    • , Lulin Li
    • , Huilong Ma
    • , Jian Luo
    • , Yongye Liang
    • , Wen Jung Li
    • , Guosong Hong
    • , Lianqing Liu
    •  & Hongjie Dai
  • Protocols |

    This protocol for clearing and high-resolution 3D imaging of entire organoids expressing fluorescence reporters or following immunolabeling enables confocal, super-resolution confocal, multiphoton and light-sheet microscopy to be performed.

    • Johanna F. Dekkers
    • , Maria Alieva
    • , Lianne M. Wellens
    • , Hendrikus C. R. Ariese
    • , Paul R. Jamieson
    • , Annelotte M. Vonk
    • , Gimano D. Amatngalim
    • , Huili Hu
    • , Koen C. Oost
    • , Hugo J. G. Snippert
    • , Jeffrey M. Beekman
    • , Ellen J. Wehrens
    • , Jane E. Visvader
    • , Hans Clevers
    •  & Anne C. Rios
  • Research | | open

    3D single molecule localization microscopy suffers from several experimental biases that degrade the resolution or localization precision. Here the authors present a dual-view detection scheme combining supercritical angle fluorescence and astigmatic imaging to obtain precise and unbiased 3D super resolution images.

    • Clément Cabriel
    • , Nicolas Bourg
    • , Pierre Jouchet
    • , Guillaume Dupuis
    • , Christophe Leterrier
    • , Aurélie Baron
    • , Marie-Ange Badet-Denisot
    • , Boris Vauzeilles
    • , Emmanuel Fort
    •  & Sandrine Lévêque-Fort

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