Flow cytometry articles from across Nature Portfolio

Real-time lab analysis is key to support clinical research during space missions. Here, the authors show scant test samples can be measured in microgravity using a miniature cytometery-based analyzer, the rHEALTH ONE with specific spaceflight modifications.

Barcoding individual cells with microparticles emitting near-infrared laser light enables the use of flow cytometry for the measurement of time-resolved single-cell dynamics with more markers and fewer colours.

A new study combining experimental treatments of human blood cells from thousands of individuals with flow-cytometry-based phenotyping and then genome-wide association analyses identifies genetic loci associated with non-resting cell states. Integrating the results with disease association signals yields insights into the underlying biology.

Barcoding cells with microparticles that emit near-infrared laser light enables the use of flow cytometry to track the dynamics of single cells by using more markers and fewer colours.

High-dimensional cytometry experiments measuring 20–50 cellular markers have become routine in many laboratories. The increased complexity of these datasets requires added rigor during the experimental planning and the subsequent manual and computational data analysis to avoid artefacts and misinterpretation of results. Here we discuss pitfalls frequently encountered during high-dimensional cytometry data analysis and aim to provide a basic framework and recommendations for reporting and analyzing these datasets.

For genome-wide screens and other applications that require the processing of a large number of cells, the immunomagnetic sorting of cells on a microfluidic chip is a scalable, rapid and cost-efficient alternative to fluorescence-activated cell sorting.