Endocytosis

Endocytosis is the internalisation of extracellular material. Endocytic pathways include clathrin-dependent endocytosis, caveolae-dependent endocytosis, macropinocytosis and phagocytosis. Endosomes that are generated by endocytosis can be recycled back to the plasma membrane, targeted to another subcellular location, or sent to multivesicular bodies for degradation.

Latest Research and Reviews

  • Research
    | Open Access

    Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.

    • Laura I. FitzGerald
    • , Luigi Aurelio
    • , Moore Chen
    • , Daniel Yuen
    • , Joshua J. Rennick
    • , Bim Graham
    •  & Angus P. R. Johnston
  • Research
    | Open Access

    Receptor tyrosine kinase mutations are frequent and associated with poor prognosis in acute myeloid leukemia (AML). Here the authors show that the antipsychotic drug chlorpromazine reduces AML cells viability by perturbing the intracellular localization of FLT3-ITD and KIT-D816V.

    • Shinya Rai
    • , Hirokazu Tanaka
    • , Mai Suzuki
    • , J. Luis Espinoza
    • , Takahiro Kumode
    • , Akira Tanimura
    • , Takafumi Yokota
    • , Kenji Oritani
    • , Toshio Watanabe
    • , Yuzuru Kanakura
    •  & Itaru Matsumura
  • Protocols |

    This protocol describes a procedure for live-cell imaging of endocytic events in cultured cells using a pH-sensitive fluorophore and fast extracellular pH changes. A MATLAB-based analysis pipeline is provided to facilitate automated data processing.

    • Silvia Sposini
    • , Morgane Rosendale
    • , Léa Claverie
    • , Thi Nhu Ngoc Van
    • , Damien Jullié
    •  & David Perrais
    Nature Protocols 15, 3088-3104
  • Research
    | Open Access

    Endothelial cell (EC) dysfunction and inflammation contribute to plaque destabilization in atherosclerosis, increasing the risk of thrombotic events. Here, the authors show that epsin promotes EC inflammation via a mechanism involving IP3R1 degradation, and that deletion of epsin in the endothelium prevents EC dysfunctoin and atherosclerosis in mice.

    • Yunzhou Dong
    • , Yang Lee
    • , Kui Cui
    • , Ming He
    • , Beibei Wang
    • , Sudarshan Bhattacharjee
    • , Bo Zhu
    • , Tadayuki Yago
    • , Kun Zhang
    • , Lin Deng
    • , Kunfu Ouyang
    • , Aiyun Wen
    • , Douglas B. Cowan
    • , Kai Song
    • , Lili Yu
    • , Megan L. Brophy
    • , Xiaolei Liu
    • , Jill Wylie-Sears
    • , Hao Wu
    • , Scott Wong
    • , Guanglin Cui
    • , Yusuke Kawashima
    • , Hiroyuki Matsumoto
    • , Yoshio Kodera
    • , Richard J. H. Wojcikiewicz
    • , Sanjay Srivastava
    • , Joyce Bischoff
    • , Da-Zhi Wang
    • , Klaus Ley
    •  & Hong Chen
  • Research |

    CD44 is a cell-surface adhesion receptor associated with many biological processes that rely on cellular plasticity. Now, CD44 has been shown to mediate endocytosis of iron-bound hyaluronates. Furthermore, iron catalyses the demethylation of repressive histone marks, thereby unlocking the expression of genes regulating cellular plasticity.

    • Sebastian Müller
    • , Fabien Sindikubwabo
    • , Tatiana Cañeque
    • , Anne Lafon
    • , Antoine Versini
    • , Bérangère Lombard
    • , Damarys Loew
    • , Ting-Di Wu
    • , Christophe Ginestier
    • , Emmanuelle Charafe-Jauffret
    • , Adeline Durand
    • , Céline Vallot
    • , Sylvain Baulande
    • , Nicolas Servant
    •  & Raphaël Rodriguez
    Nature Chemistry 12, 929-938

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