Embryonic stem cells

Embryonic stem cells are pluripotent cells isolated from the inner cell mass of a blastocyst, the early mammalian embryo that implants into the uterus. Embryonic stem cells self-renew by dividing and can differentiate into any specialised cell of the body, but not extra-embryonic tissues such as the placenta.

Latest Research and Reviews

  • Research |

    Daneshvar et al. show that BRD3 and the lncRNA DIGIT form phase-separated condensates at genomic regions with H3K18ac to co-regulate the transcription of genes that drive endoderm differentiation.

    • Kaveh Daneshvar
    • , M. Behfar Ardehali
    • , Isaac A. Klein
    • , Fu-Kai Hsieh
    • , Arcadia J. Kratkiewicz
    • , Amin Mahpour
    • , Sabrina O. L. Cancelliere
    • , Chan Zhou
    • , Brett M. Cook
    • , Wenyang Li
    • , Joshua V. Pondick
    • , Sweta K. Gupta
    • , Sean P. Moran
    • , Richard A. Young
    • , Robert E. Kingston
    •  & Alan C. Mullen
  • Research
    | Open Access

    Rohani et al show that converting human pluripotent stem cells (hPSCs) to the naïve state enhances hPSC manufacturing. Further, through transcriptomics, the expression of epigenetic regulators, metabolomics, and cell-surface protein marker analyses, they indicate a potentially important role for the stirred bioreactor’s mechanical environment in maintaining naïve-like pluripotency.

    • Leili Rohani
    • , Breanna S. Borys
    • , Golsa Razian
    • , Pooyan Naghsh
    • , Shiying Liu
    • , Adiv A. Johnson
    • , Pranav Machiraju
    • , Heidrun Holland
    • , Ian A. Lewis
    • , Ryan A. Groves
    • , Derek Toms
    • , Paul M. K. Gordon
    • , Joyce W. Li
    • , Tania So
    • , Tiffany Dang
    • , Michael S. Kallos
    •  & Derrick E. Rancourt
  • Research
    | Open Access

    Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction.

    • Dounia Djeghloul
    • , Bhavik Patel
    • , Holger Kramer
    • , Andrew Dimond
    • , Chad Whilding
    • , Karen Brown
    • , Anne-Céline Kohler
    • , Amelie Feytout
    • , Nicolas Veland
    • , James Elliott
    • , Tanmay A. M. Bharat
    • , Abul K. Tarafder
    • , Jan Löwe
    • , Bee L. Ng
    • , Ya Guo
    • , Jacky Guy
    • , Miles K. Huseyin
    • , Robert J. Klose
    • , Matthias Merkenschlager
    •  & Amanda G. Fisher
  • Research
    | Open Access

    Epigenetic reprogramming is a hallmark of cancer. Here the authors find that resetting primed human embryonic stem cells to naïve state results in the acquisition of a DNA methylation landscape that mirrors the cancer DNA methylome and provides evidence that the transition to naïve pluripotency and oncogenic transformation share common epigenetic trajectories.

    • Hemalvi Patani
    • , Michael D. Rushton
    • , Jonathan Higham
    • , Saul A. Teijeiro
    • , David Oxley
    • , Pedro Cutillas
    • , Duncan Sproul
    •  & Gabriella Ficz

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