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The enzyme-linked immunosorbent spot (ELISPOT) assay is a sensitive method to count cells of a specific type by capturing molecules that they secrete on an immunosorbent surface and detecting them with the ELISA method. It is commonly used to count immune cells based on the secretion of cytokines.
Time-Dependent Cell-State Selection combines live cell imaging and single-cell RNA sequencing to characterize and analyse ILC2s during their activation, revealing a set of genes that are transitionally upregulated (TIGs) in ILC2s during this phase.
Understanding the immune response to SARS-CoV-2 is dependent on being able to distinguish COVID-19 immune responses from cross-reactive immune responses to other coronaviruses. Here the authors show that choice of antigens and whether an ICS, ELISPOT or T cell proliferation assay is used has a major effect on this discriminatory ability.
This protocol summarizes the recommendations developed after a comparison of Elispot plate-reading approaches by a panel of laboratories and subsequent agreement regarding the optimal steps to follow when evaluating Elispot plates.
