DNA replication

DNA replication is the biological process by which an exact copy of a deoxyribonucleic acid (DNA) molecule is created and it is the basis for biological inheritance. Each of the two strands of the DNA molecule acts as a template for the synthesis of a complementary strand.

Latest Research and Reviews

  • Research
    | Open Access

    In metazoan the DNA sequence elements characterizing origin specification are unknown. By generating and analysing 19 SNS-seq datasets from different human cell types, the authors reveal a class and features of Core origins of replication which can be predicted by an algorithm.

    • Ildem Akerman
    • , Bahar Kasaai
    • , Alina Bazarova
    • , Pau Biak Sang
    • , Isabelle Peiffer
    • , Marie Artufel
    • , Romain Derelle
    • , Gabrielle Smith
    • , Marta Rodriguez-Martinez
    • , Manuela Romano
    • , Sandrina Kinet
    • , Peter Tino
    • , Charles Theillet
    • , Naomi Taylor
    • , Benoit Ballester
    •  & Marcel Méchali
  • Research
    | Open Access

    In mammalian cells, during transcription and replication, RNA:DNA hybrid structures known as R-loops can arise, posing as obstacles to replication fork progression. Here the authors reveal that the ATP-dependent chromatin remodelling INO80 complex promotes resolution of R-loops to prevent replication associated DNA damage in cancer cells.

    • Lisa Prendergast
    • , Urszula L. McClurg
    • , Rossitsa Hristova
    • , Rolando Berlinguer-Palmini
    • , Sarah Greener
    • , Katie Veitch
    • , Inmaculada Hernandez
    • , Philippe Pasero
    • , Daniel Rico
    • , Jonathan M. G. Higgins
    • , Anastas Gospodinov
    •  & Manolis Papamichos-Chronakis
  • Research
    | Open Access

    A unique cell envelope contributes to the antibiotic resistance of the pathogen Acinetobacter baumannii. Here, Geisinger et al. identify A. baumannii mutants with altered antibiotic susceptibility, infer the function of uncharacterized proteins involved in envelope synthesis, and predict antibiotic synergies.

    • Edward Geisinger
    • , Nadav J. Mortman
    • , Yunfei Dai
    • , Murat Cokol
    • , Sapna Syal
    • , Andrew Farinha
    • , Delaney G. Fisher
    • , Amy Y. Tang
    • , David W. Lazinski
    • , Stephen Wood
    • , Jon Anthony
    • , Tim van Opijnen
    •  & Ralph R. Isberg
  • Research
    | Open Access

    WABS patient derived cells display loss of sister chromatid cohesion. Here the authors by analyzing WABS patient derived cells, reveal a role of the DDX11 helicase in resolving G-Quadruplex structures to support sister chromatid cohesion.

    • Janne J. M. van Schie
    • , Atiq Faramarz
    • , Jesper A. Balk
    • , Grant S. Stewart
    • , Erika Cantelli
    • , Anneke B. Oostra
    • , Martin A. Rooimans
    • , Joanna L. Parish
    • , Cynthia de Almeida Estéves
    • , Katja Dumic
    • , Ingeborg Barisic
    • , Karin E. M. Diderich
    • , Marjon A. van Slegtenhorst
    • , Mohammad Mahtab
    • , Francesca M. Pisani
    • , Hein te Riele
    • , Najim Ameziane
    • , Rob M. F. Wolthuis
    •  & Job de Lange
  • Research
    | Open Access

    The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its cofactor Cdc6 and also report an in vitro reconstitution system for Drosophila Mcm2–7 loading, revealing unexpected features of ORC’s DNA binding and remodeling mechanism during Mcm2–7 loading.

    • Jan Marten Schmidt
    •  & Franziska Bleichert
  • Research
    | Open Access

    Mycobacteria Prim-PolC performs short gap synthesis following removal of lesions during excision repair. Here the authors resolve crystal structures of pre- and post-catalytic Prim-PolC complexes bound to gapped DNA substrates to define its mechanism.

    • Nigel C. Brissett
    • , Katerina Zabrady
    • , Przemysław Płociński
    • , Julie Bianchi
    • , Małgorzata Korycka-Machała
    • , Anna Brzostek
    • , Jarosław Dziadek
    •  & Aidan J. Doherty

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