Cryoelectron tomography articles from across Nature Portfolio

Using cryo-electron tomography, Dendooven et al. determined the structure of the native budding yeast γ-tubulin ring complex (γTuRC) capping spindle microtubules and showed that γTuRC adopts an active closed conformation to function as a perfect geometric template for microtubule nucleation.

It can be challenging to obtain meaningful and accurate structural information for air-sensitive proteins. This protocol describes the application of customized vacuum manifold and anaerobic chamber setups for the purification and cryo-electron microscopy analysis of air-sensitive nitrogenase enzymes.

The interplay between cryo-electron microscopy and cryo-electron tomography to define complex macromolecular assemblies and visualize them in situ is explored.

Here the authors report a method for cryogenic electron microscopy imaging of human brain tissue samples directly obtained from autopsy, offering insights into cellular ultrastructure and a tool to study potential pathologic features.

Square and rectangular beams made with new condenser apertures enable more efficient image capture in low-dose transmission electron microscopy with no compromise to image quality in single-particle cryoelectron microscopy.

TomoDRGN is a deep learning framework designed to model conformational and compositional heterogeneity from cryo-ET datasets on a per-particle basis.

Advances will enable proteome-scale structure determination in cells.

To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.

Sperm flagella of highly divergent eukaryotic species share an architectural plan. Despite their ostensible ultrastructural similarities, mammalian sperm flagella beat with an asymmetric waveform, in contrast to the symmetrical beats of other eukaryotic flagella. Structural findings elucidate the molecular basis for this evolutionary divergence.