Confocal microscopy articles from across Nature Portfolio

Here, the authors describe a pathogenic fungus from a 400-million-year-old fossil plant from the Devonian Rhynie Chert in Scotland. They use advanced imaging methods to determine that the fungus belongs to the sac fungi, the most diverse group of Fungi today.

Mie resonances are typically manipulated through varying nanostructure shape/size. Here, authors found that Gaussian beam displacement excites higher-order multipolar modes, not accessible by plane wave, featuring maximal linear and nonlinear scattering efficiency when the focus is misaligned.

The prevalence of centrosome amplification (CA) and the genomic landscape of chromosomal instability in high-grade serous ovarian carcinoma (HGSOC) remain to be explored. Here the authors suggest CA as a potential driver of tumour evolution and a biomarker for treatment response in HGSOC.

The optimized and disordered structures of planar diffractive lenses enable sub-diffraction limit focusing but destroy wide-field imaging. Here, the authors introduce the information entropy to evaluate the disorder of PDL and achieve good balance between super-focusing and imaging.

Microscopic visualization of the ARP2/3 complex showed that it colocalizes with peroxisomes in Arabidopsis thaliana and tobacco cells in vivo. Colocalization with an autophagy marker and analysis of peroxisomes in autophagy-mutant or ARP2/3-mutant lines demonstrated that ARP2/3 facilitates peroxisome degradation by the autophagic pathway, that is, pexophagy.

Researchers push the limits of confocal microscopy by combining multiple views, super-resolution and deep learning.

Sharing, teaching and singing to bring people together on microscopy standards.