Cloning articles within Nature Communications

Featured

• Article
  16 January 2024 | Open Access

  Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer

  Somatic cloning of rhesus monkey has not been successful until now. Here, authors report epigenetic abnormalities in SCNT embryos and placentas and develop a trophoblast replacement method that enables them to successful clone of a healthy male rhesus monkey.

  • Zhaodi Liao
  • , Jixiang Zhang
  •  & Qiang Sun

• Article
  26 July 2016 | Open Access

  Healthy ageing of cloned sheep

  Since the birth of the first cloned animal, Dolly the sheep, concerns have been raised about potential long-term health consequences of cloning. Here the authors report on a cohort of 13 aged cloned sheep, including four created from the same cells as Dolly, and find they are healthy and seem to age normally.

  • K. D. Sinclair
  • , S. A. Corr
  •  & D. S. Gardner

• Article
  01 September 2015 | Open Access

  Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

  Genomic engineering often requires the cloning of long DNA segments that contain large gene clusters. Here, the authors describe an RNA-guided Cas9 nuclease assistedin vitrotechnique that allows the targeted cloning of near-arbitrary, long bacterial genomic sequences of up to 100 kb in a single step.

  • Wenjun Jiang
  • , Xuejin Zhao
  •  & Ting F. Zhu