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| Open AccessPEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation
It is still a challenge to accurately identify off-target endonuclease edits. Here the authors report PEAC-seq using a Prime Editor to insert a tag to the editing sites and enrich the tagged regions with site-specific primers for sequencing: they show that PEAC-seq could identify DNA translocations.
- Zhenxing Yu
- , Zhike Lu
- & Lijia Ma
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| Open AccessMolecular signatures of aneuploidy-driven adaptive evolution
Aneuploidy (abnormal chromosome number) can enable rapid adaptation to stress conditions, but it also entails fitness costs from gene imbalance. Here, the authors experimentally evolve yeast while forcing maintenance of aneuploidy to identify the mechanisms that promote tolerance of aneuploidy.
- Alaattin Kaya
- , Marco Mariotti
- & Vadim N. Gladyshev
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| Open AccessTrisomy 21 activates the kynurenine pathway via increased dosage of interferon receptors
Down syndrome (DS) is caused by trisomy 21 (T21), but the underlying etiology of the related immune and neurological dysfunction is unclear. Here, the authors show that T21 activates the kynurenine pathway via increased interferon receptor copy number, which could contribute to DS pathophysiology.
- Rani K. Powers
- , Rachel Culp-Hill
- & Joaquin M. Espinosa
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| Open AccessGWAS of mosaic loss of chromosome Y highlights genetic effects on blood cell differentiation
Mosaic loss of chromosome Y (mLOY) is associated with age and smoking but also genetic factors play a role. Here, Terao et al. perform GWAS for mLOY in 95,380 Japanese men and identify 46 loci that overlap with hematopoietic stem cell enhancers and transcription factor binding sites critical for hematopoiesis.
- Chikashi Terao
- , Yukihide Momozawa
- & Yoichiro Kamatani
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| Open AccessDistinct adaptive mechanisms drive recovery from aneuploidy caused by loss of the Ulp2 SUMO protease
Transient aneuploidy enables cells to survive sudden environmental changes before longterm cellular adaptations are established. Here, the authors show that yeast cells respond to the acute loss of Ulp2 SUMO protease by rapid induction of aneuploidy, and reveal predictable long-term adaptation mechanisms that restore euploidy.
- Hong-Yeoul Ryu
- , Francesc López-Giráldez
- & Mark Hochstrasser
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| Open AccessFoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence
Evidence for mitotic decline in aged cells and for aneuploidy-driven progression into full senescence is limited. Here, the authors find that in aged cells, mitotic gene repression leads to increased chromosome mis-segregation and aneuploidy that triggers permanent cell cycle arrest and full senescence.
- Joana Catarina Macedo
- , Sara Vaz
- & Elsa Logarinho
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| Open AccessSystematic proteome and proteostasis profiling in human Trisomy 21 fibroblast cells
Trisomy 21 (T21) is a major cause of Down syndrome but little is known about its impact on the cellular proteome. Here, the authors define the proteome of T21 fibroblasts and its turnover and also map proteomic differences in monozygotic T21-discordant twins, revealing extensive, organelle-specific changes caused by T21.
- Yansheng Liu
- , Christelle Borel
- & Ruedi Aebersold
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| Open AccessAnalysis of chromosomal aberrations and recombination by allelic bias in RNA-Seq
Chromosomal aberrations can be detected by global gene expression analysis. Here, the authors report eSNP-Karyotyping, a new method that can detect chromosomal aberrations by measuring the ratio of expression between the two alleles without comparison to a matched diploid sample.
- Uri Weissbein
- , Maya Schachter
- & Nissim Benvenisty
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| Open AccessThe landscape of chromosomal aberrations in breast cancer mouse models reveals driver-specific routes to tumorigenesis
Genetically engineered mouse models of cancer are useful in identifying oncogenes and tumour suppressors. Here, the authors use gene expression profiles to generate a catalogue of copy number aberrations in 45 mouse models, and compare mouse and human tumours to identify additional drivers of tumorigenesis.
- Uri Ben-David
- , Gavin Ha
- & Todd R. Golub
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| Open AccessFemale chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome
It is unclear how often genetic mosaicism of chromosome X arises. Here, the authors examine women with cancer and cancer-free controls and show that X chromosome mosaicism occurs more frequently than on autosomes, especially on the inactive X chromosome, but is not linked to non-haematologic cancer risk
- Mitchell J. Machiela
- , Weiyin Zhou
- & Stephen J. Chanock
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| Open AccessMouse model of chromosome mosaicism reveals lineage-specific depletion of aneuploid cells and normal developmental potential
The developmental potential of mosaic embryos of euploid and aneuploid cells is unknown. Here, the authors create a mouse model of chromosome mosaicism, showing that aneuploid cells in the fetus are eliminated by apoptosis and developmental potential is dependent on the presence of sufficient euploid cells.
- Helen Bolton
- , Sarah J. L. Graham
- & Magdalena Zernicka-Goetz
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| Open AccessThe presence of extra chromosomes leads to genomic instability
One of the hallmarks of cancer cells is aneuploidy, however the molecular effects are poorly understood. Here the authors show that trisomic and tetrasomic cells display increased genomic instability and reduced levels of the helicase MCM2-7.
- Verena Passerini
- , Efrat Ozeri-Galai
- & Zuzana Storchová
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| Open AccessNSD1 mutations generate a genome-wide DNA methylation signature
Sotos syndrome is an growth syndrome characterized by advanced growth in childhood, characteristic facial appearance and intellectual disability. Here the authors identify a genome-wide DNA methylation signature that accurately diagnoses Sotos Syndrome and distinguishes it from similar conditions.
- S. Choufani
- , C. Cytrynbaum
- & R. Weksberg
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| Open AccessAF4 uses the SL1 components of RNAP1 machinery to initiate MLL fusion- and AEP-dependent transcription
Protein fusions between MLL and AEP (AF4 family/ENL family/P-TEFb) constitutively activate their target genes to immortalize hematopoietic progenitors. Here, Okuda et al. show that MLL-AEP binds SL1, a component of the pre-initiation complex of RNA polymerase (RNAP) I, to initiate RNAP II dependent transcription.
- Hiroshi Okuda
- , Akinori Kanai
- & Akihiko Yokoyama
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| Open AccessAneuploidy causes premature differentiation of neural and intestinal stem cells
It is unclear why certain tissues are more susceptible to the consequences of aneuploidy. Here, in Drosophila, Gogendeau et al.identify aneuploidy as the cause of lengthened G1 and premature differentiation in both neural and adult intestinal stem cells, which prevents cells with abnormal genomes from cycling.
- Delphine Gogendeau
- , Katarzyna Siudeja
- & Renata Basto
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| Open AccessMutagenic consequences of a single G-quadruplex demonstrate mitotic inheritance of DNA replication fork barriers
Barriers to DNA replication are potent sources of genome instability. Here, the authors provide a mechanistic model for how a single persistent G-quadruplex structure generates multiple substrates for polymerase theta-mediated end-joining, thus causing multiple deletions during animal development.
- Bennie Lemmens
- , Robin van Schendel
- & Marcel Tijsterman
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| Open AccessCell populations can use aneuploidy to survive telomerase insufficiency
The loss of telomeres is a catastrophic event and eukaryotes have evolved multiple strategies to overcome this. Here the authors show that Saccharomyces cerevisiaecan generate aneuploid survivors that upregulate telomerase to overcome telomere loss.
- Caroline Millet
- , Darya Ausiannikava
- & Svetlana Makovets
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| Open AccessThe DNA structure and sequence preferences of WRN underlie its function in telomeric recombination events
The loss of WRN helicase leads to abnormalities at chromosome ends and is associated with premature ageing phenotypes characteristic of Werner syndrome. Here the authors show that WRN acts in a structure- and sequence-specific manner on recombination intermediates relevant to telomere maintenance.
- Deanna N. Edwards
- , Amrita Machwe
- & David K. Orren
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| Open AccessMulticohort analysis of the maternal age effect on recombination
The question of whether recombination rate increases with maternal age is controversial, with conflicting prior evidence. Here, Martin et al.analyse nine cohorts in the largest SNP-based analysis of this question and find a small positive increase with maternal age in the number of crossovers.
- Hilary C. Martin
- , Ryan Christ
- & Peter Donnelly
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| Open AccessPrediction model for aneuploidy in early human embryo development revealed by single-cell analysis
Aneuploidy may be fatal for the embryo, hence predicting its occurrence is important for successfulin vitrofertilization. Here the authors monitor development of human preimplantation embryos in real-time and correlate the blastomere ploidy with cleavage dynamics and gene expression, identifying 12-transcript signature that determines ploidy.
- Maria Vera-Rodriguez
- , Shawn L. Chavez
- & Carlos Simon
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| Open AccessAneuploidy generates proteotoxic stress and DNA damage concurrently with p53-mediated post-mitotic apoptosis in SAC-impaired cells
CENP-E regulates chromosome alignment during mitosis to distribute chromosomes equally into daughter cells. Here, the authors show that CENP-E inhibition causes p53-mediated post-mitotic apoptosis in tumours where the spindle assembly checkpoint is compromised, suggesting that CENP-E is a therapeutic target for these cancers.
- Akihiro Ohashi
- , Momoko Ohori
- & Kentaro Iwata
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| Open AccessBivalent separation into univalents precedes age-related meiosis I errors in oocytes
As oocytes age the frequency of chromosome segregation errors during meiosis I increases. Here the authors use live imaging of oocytes from naturally aged mice to provide direct evidence that bivalent separation into univalents is the primary defect responsible for age-related aneuploidy.
- Yogo Sakakibara
- , Shu Hashimoto
- & Tomoya S. Kitajima
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Loss of kinesin-14 results in aneuploidy via kinesin-5-dependent microtubule protrusions leading to chromosome cut
Loss of the motor protein kinesin-14 can lead to aneuploidy, but the mechanism is not known. Here the authors show that loss of kinesin-14 in fission yeast leads to long spindle microtubule protrusions that push properly segregated chromosomes into the division site, leading to chromosome cut during cytokinesis.
- Viktoriya Syrovatkina
- & Phong T. Tran
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APOBEC family mutational signatures are associated with poor prognosis translocations in multiple myeloma
Rearrangements of the Ig loci are essential for generating antibody diversity but abnormal translocations can be a driving event for myeloma. Here Walker et al. perform whole exome sequencing on myeloma patients to capture the diversity of mutational changes.
- Brian A. Walker
- , Christopher P. Wardell
- & Gareth J. Morgan
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Numerical chromosomal instability mediates susceptibility to radiation treatment
Ionizing radiations (IRs) cause widespread genomic damage and can, through unknown mechanisms, lead to changes in chromosome numbers by perturbing the cells undergoing mitosis. Here, the authors investigate the potential mechanism behind the increased susceptibility of mitotic cells to IRs.
- Samuel F. Bakhoum
- , Lilian Kabeche
- & Duane A. Compton
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Aneuploidy induces profound changes in gene expression, proliferation and tumorigenicity of human pluripotent stem cells
Trisomy 12 is the most frequent chromosomal abnormality detected in cultures of human pluripotent stem cells. Here the authors show that human pluripotent stem cells carrying this abnormality exhibit gene expression profiles more similar to those of germ cell tumours, and give rise to more aggressive teratomas.
- Uri Ben-David
- , Gal Arad
- & Juan Carlos Biancotti
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Merotelic attachments allow alignment and stabilization of chromatids in meiosis II oocytes
Chromosome mis-segregation is a frequent occurrence in meiosis. Here the authors show that single unpaired chromatids avoid the spindle assembly checkpoint in the second meiotic division as they form bi-directional microtubule–kinetochore attachments, which leads to the completion of meiosis and the establishment of aneuploidy.
- Anna Kouznetsova
- , Abrahan Hernández-Hernández
- & Christer Höög
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Low-grade chromosomal mosaicism in human somatic and embryonic stem cell populations
De novocopy number variations are known to occur in somatic cell populations and pluripotent stem cells. Here the authors use single-cell array comparative genomic hybridization to identify copy number variations in individual human somatic and embryonic stem cells.
- Kurt Jacobs
- , Afroditi Mertzanidou
- & Claudia Spits
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Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR–Cas9 system
CRISPR and Cas9 are endonucleases that are found in bacteria and have recently been exploited for genome engineering. Here, the authors use this system in cultured mammalian cells to engineer chromosomal translocations that are found in acute myeloid leukaemia and Ewing’s sarcoma.
- R. Torres
- , M. C. Martin
- & S. Rodriguez-Perales
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Non-canonical function of spindle assembly checkpoint proteins after APC activation reduces aneuploidy in mouse oocytes
The spindle assembly checkpoint (SAC) has been viewed as a switch that prevents chromosome segregation until all chromosomes are correctly attached to spindle microtubules. Lane and Jones show that in meiosis I, SAC proteins remain partially active after prometaphase, and prevent aneuploidy by prolonging meiosis.
- Simon I.R. Lane
- & Keith T. Jones
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Two sequential cleavage reactions on cruciform DNA structures cause palindrome-mediated chromosomal translocations
Palindromic DNA sequences in the genome can cause gross chromosomal rearrangements. Inagaki et al.demonstrate how the pathways of Holliday-junction resolution and antigen-receptor gene rearrangement interact to process cruciform conformation of palindrome DNA into chromosomal translocations in human embryonic kidney cells.
- Hidehito Inagaki
- , Tamae Ohye
- & Hiroki Kurahashi
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| Open AccessDynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage
Abnormal human embryo development is implicated in the embryo arrest observed during in vitrofertilization. Chavez and colleagues perform time-lapse imaging on human embryos and find that chromosomally abnormal embryos exhibit diverse cell cycle parameters that may contribute to arrest.
- Shawn L. Chavez
- , Kevin E. Loewke
- & Renee A. Reijo Pera