Cell migration

  • Article
    | Open Access

    It is unclear how bacterial cells adapt the reversible switching of flagellar motor rotation to environments of different viscosities. Here, Antani et al. show that flagellar mechanosensors allosterically control the motor’s binding affinity for the chemotaxis response regulator, CheY-P, to adapt flagellar switching over varying viscous loads.

    • Jyot D. Antani
    • , Rachit Gupta
    •  & Pushkar P. Lele
  • Article
    | Open Access

    The assembly of branched actin networks depends on the heterodimeric capping protein CP/CapZ. Combining cryoEM, in vitro reconstitution and cell biological assays, the authors show that CP not only prevents actin filament elongation but also selectively masks actin filament ends to promote nucleation.

    • Johanna Funk
    • , Felipe Merino
    •  & Peter Bieling
  • Article
    | Open Access

    Cells locally expand and retract their surface in response to environmental factors such as changes in membrane tension. Here the authors show the membrane adapter, dynamin2, locally constricts surface membrane to form an isolated but contiguous membrane reservoir that can open upon phospholipid scrambling via TMEM16F.

    • Christine Deisl
    • , Donald W. Hilgemann
    •  & Michael Fine
  • Article
    | Open Access

    Environmental and genetic risk factors affect the distal airway epithelium in idiopatic pulmonary fibrosis (IPF) but the role of the epithelium in IPF remains unclear. Here the authors show that pathologic activation of the ERBB-YAP axis induces dynamic and structural dysfunction in the distal airway epithelium eliciting a pro-fibrotic phenotype in mesenchymal cells.

    • Ian T. Stancil
    • , Jacob E. Michalski
    •  & David A. Schwartz
  • Article
    | Open Access

    Cells can modify their environment by depositing biochemical signals or mechanically remodelling the extracellular matrix; the impact of such self-induced environmental perturbations on cell trajectories at various scales remains unexplored. Here authors show that motile cells leave long-lived physicochemical footprints along their way, which determine their future path.

    • Joseph d’Alessandro
    • , Alex Barbier--Chebbah
    •  & Benoît Ladoux
  • Article
    | Open Access

    Myosin II–mediated contractility is required for leukocyte migration. Here, authors show that lysosomes are involved in leukocyte migration by providing the platform where Ragulator complex interacts with the myosin phosphatase Rho-interacting protein (MPRIP) independently of mTORC1 and interferes with the interaction between MPRIP and a subunit of myosin light chain phosphatase (MLCP).

    • Takeshi Nakatani
    • , Kohei Tsujimoto
    •  & Atsushi Kumanogoh
  • Article
    | Open Access

    Communication between endothelial leader and follower cells during collective cell migration is crucial for vascular development. Here, the authors show that PACSIN2 guides collective cell migration and angiogenesis by recruiting a protein trafficking complex to asymmetric cell-cell junctions, controlling local junction plasticity.

    • Tsveta S. Malinova
    • , Ana Angulo-Urarte
    •  & Stephan Huveneers
  • Article
    | Open Access

    The absence of scaffold protein Ambra1 leads to hyperproliferation and growth in mouse models. Here the authors show that Ambra1 deficiency accelerates melanoma growth and increases metastasis in mouse models of melanoma through FAK1 hyperactivation.

    • Luca Di Leo
    • , Valérie Bodemeyer
    •  & Francesco Cecconi
  • Article
    | Open Access

    Contact stimulation of migration drives tissue morphogenesis. Here the authors report that filopodia-based contact-dependent asymmetry of cell–matrix adhesion drives directional movement, whereas contractile actin cables contribute to the integrity of the migrating cell cluster in the myotubes of Drosophila developing testes.

    • Maik C. Bischoff
    • , Sebastian Lieb
    •  & Sven Bogdan
  • Article
    | Open Access

    The cytoplasm in mammalian cells is considered homogeneous. Here authors report that the cytoplasmic fluidity is regulated in the blebbing cells, which is regulated by calcium concentration in the expanding blebs and involves the STIM-Orai1 pathway.

    • Kana Aoki
    • , Shota Harada
    •  & Junichi Ikenouchi
  • Article
    | Open Access

    Mesodermal directional cell migration is needed to establish body plan but how this is regulated is unclear. Here, the authors show that loss of the guanine nucleotide exchange factor for Rac1 and Cdc42, β-Pix, at mouse gastrulation disrupts the orderly, collective anterior migration of mesoderm cells due to defective cell protrusions.

    • Tatiana Omelchenko
    • , Alan Hall
    •  & Kathryn V. Anderson
  • Article
    | Open Access

    The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology. Here, the authors use a combination of live cell imaging and in vitro single molecule binding measurements to show that tandem calponin homology domains (CH1–CH2) are sensitive to actin filament conformation, biasing their subcellular localization.

    • Andrew R. Harris
    • , Pamela Jreij
    •  & Daniel A. Fletcher
  • Article
    | Open Access

    Breakdown of vascular barriers is a major complication of inflammatory diseases. However, the mechanisms underlying platelet recruitment to inflammatory micro-environments remains unclear. Here, the authors identify haptotaxis as a key effector function of immune-responsive platelets

    • Leo Nicolai
    • , Karin Schiefelbein
    •  & Florian Gaertner
  • Article
    | Open Access

    In neural development, progenitors transition from a proliferative to a differentiated state. Here, the authors show that cerebellar granule neurons retract primary cilia as they exit their proliferative niche upon decreased ECM engagement, enabling radial migration due to loss of Shh sensitivity.

    • Taren Ong
    • , Niraj Trivedi
    •  & David J. Solecki
  • Article
    | Open Access

    During repair, development, or cancer metastasis, epithelial cells can become migratory through partial or full epithelial to mesenchymal transition (EMT). Here, the authors report that differentiated epithelial collectives may undergo cooperative and collective migration without evidence of partial EMT through an unjamming transition (UJT).

    • Jennifer A. Mitchel
    • , Amit Das
    •  & Jin-Ah Park
  • Article
    | Open Access

    In vivo, cells migrate across a diverse landscape of extracellular matrix containing gaps which present a challenge for cells to protrude across. Here, the authors show that T-Plastin strengthens protrusive actin networks to promote protrusion, extracellular matrix gap-bridging, and cell migration.

    • Damien Garbett
    • , Anjali Bisaria
    •  & Tobias Meyer
  • Article
    | Open Access

    During development, primordial germ cell clusters undergo dispersal but how cell–cell adhesion and contractility are coordinated during this process in vivo is unclear. Here, the authors show that Drosophila primordial germ cells utilize migratory forces to disperse through G-protein coupled receptor mediated collective guidance of front-back polarity outwards from the cluster.

    • B. Lin
    • , J. Luo
    •  & R. Lehmann
  • Article
    | Open Access

    Migration and homing of B cells to lymph nodes are important for B cell functions, but their regulation is poorly understood. Here, the authors show that B cell-specific deletion of Cosmc results in decreased protein O-glycosylation, loss of B cell homing to both lymphoid and nonlymphoid organs, and altered transendothelial migration implicated in this loss.

    • Junwei Zeng
    • , Mahmoud Eljalby
    •  & Richard D. Cummings
  • Article
    | Open Access

    Drosophila tumours can be utilised to study the mechanisms of cell dissemination. Here, the authors use Drosophila midgut to examine the course of RasV12-transformed cell dissemination from midgut into circulation, which requires the actions of invasive protrusions and the mechanosensitive channel Piezo.

    • Jiae Lee
    • , Alejandra J. H. Cabrera
    •  & Young V. Kwon
  • Article
    | Open Access

    It is unclear if genetic alterations in endocytic proteins play a causal role in high incidence human cancers. Here, the authors report the oncogenic role of Epsin3 (EPN3) in breast cancer, and show EPN3 to drive tumorigenesis through induction of a partial epithelial mesenchymal transition state and a TGFβ-dependent regulatory loop that promotes cellular plasticity and invasive behaviour.

    • Irene Schiano Lomoriello
    • , Giovanni Giangreco
    •  & Pier Paolo Di Fiore
  • Article
    | Open Access

    During cell migration, cells are polarized with distinct front vs. rear regions but whether and how polarity is transmitted to the nucleus is unclear. Here the authors show that frontally-biased endoplasmic reticulum and the nuclear membrane protein Emerin contribute to front-rear nuclear cell polarity.

    • Paulina Nastały
    • , Divya Purushothaman
    •  & Paolo Maiuri
  • Article
    | Open Access

    The presence of phenotypic heterogeneity in collectively invading cells suggests cooperation amongst distinct subtypes of cells to promote invasion and metastasis. Here, the authors use chemical biology tools and report metabolic heterogeneity within the lung cancer collective invasion pack.

    • R. Commander
    • , C. Wei
    •  & A. I. Marcus
  • Article
    | Open Access

    Triple-negative breast cancer (TNBC) is an aggressive subtype lacking effective targeted therapies. Here, the authors show that RNF208, an estrogen-induced ubiquitin ligase, promotes the degradation of Vimentin, thereby suppressing lung metastasis of TNBC, and may serve as a biomarker for the disease.

    • Kyoungwha Pang
    • , Jinah Park
    •  & Seong-Jin Kim
  • Article
    | Open Access

    Inflammatory responses must be induced and resolved timely to serve protection from pathogens without inducing excessive tissue damage. Here the authors use live imaging in zebrafish to show that the intracellular trafficking of two chemokine receptors, Cxcr1 and Cxcr2, is differentially regulated on activated neutrophils to control their clustering and dispersal, respectively.

    • Caroline Coombs
    • , Antonios Georgantzoglou
    •  & Milka Sarris
  • Article
    | Open Access

    The mechanism of force production by invadopodia is unclear. Here, the authors show that cell surface MT1-MMP when in contact with collagen, induces Arp2/3 branched actin polymerisation on the concave side of invadopodia, which generates a pushing force along with collagen cleavage by MT1-MMP to invade.

    • Robin Ferrari
    • , Gaëlle Martin
    •  & Philippe Chavrier
  • Article
    | Open Access

    The Bin/Amphiphysin/Rvs167 (BAR) domain superfamily, which includes FCH-BAR (F-BAR) domain proteins are membrane-sculpting proteins. Here the authors combine a range of techniques and show that the F-BAR domain of growth-arrest specific protein 7 (GAS7) forms two-dimensional sheets on flat membranes and that these oligomeric assemblies of GAS7 are required for the formation of phagocytic cups in macrophages.

    • Kyoko Hanawa-Suetsugu
    • , Yuzuru Itoh
    •  & Shiro Suetsugu
  • Article
    | Open Access

    Migrating cells tune their energy utilization in response to their microenvironment, but how cellular energetics direct navigation remains unclear. Here, the authors report that energetic costs for motility, regulated by cell mechanics and confinement, predict the probability of migration choice.

    • Matthew R. Zanotelli
    • , Aniqua Rahman-Zaman
    •  & Cynthia A. Reinhart-King
  • Article
    | Open Access

    Mechanotransduction of cells is of interest for a number of reasons but model in vitro systems remain a challenge. Here, the authors report on a hydrogel which changes properties upon near infrared irradiation to create cyclic forces and demonstrate the application of these gels to study mechanotransduction.

    • Yashoda Chandorkar
    • , Arturo Castro Nava
    •  & Laura De Laporte
  • Article
    | Open Access

    Mobilization of hematopoietic stem and progenitor cells (HSPCs) into the circulation is essential for maintaining homeostasis. Here, the authors show that Phc2 in bone marrow stromal cells represses the cell adhesion molecule Vcam1 and facilitates mobilization of HSPCs through regulation of epigenetic marks.

    • Joonbeom Bae
    • , Sang-Pil Choi
    •  & Taehoon Chun
  • Article
    | Open Access

    The extracellular matrix can shape developing organs, but how external forces direct intercellular morphogenesis is unclear. Here, the authors use 3D imaging to show that elongation of the Drosophila egg chamber involves polarized cell reorientation signalled by changes in stiffness of the surrounding extracellular matrix.

    • Dong-Yuan Chen
    • , Justin Crest
    •  & David Bilder
  • Article
    | Open Access

    In both focal segmental glomerulosclerosis (FSGS) and crescentic glomerulonephritis (CGN), kidney injury is characterised by the invasion of glomerular tufts by parietal epithelial cells (PECs). Here Lazareth et al. identify the tetraspanin CD9 as a key regulator of PEC migration, and find its upregulation in FSGS and CGN contributes to kidney injury in both diseases.

    • Hélène Lazareth
    • , Carole Henique
    •  & Pierre-Louis Tharaux
  • Article
    | Open Access

    Triple-negative breast cancers (TNBC) have enhanced migratory behaviour. Here, the authors perform a phenotypic imaging-based RNAi screen to identify several genes associated with regulation of migratory phenotypes and show that one of the regulators, PRPF4B, mediates metastasis in TNBC in mice.

    • Esmee Koedoot
    • , Michiel Fokkelman
    •  & Bob van de Water