Cell growth

  • Article
    | Open Access

    A variety of signals have been reported to either activate or inhibit the Hippo kinase cascade. Here, Meng et al. show that mitogen activated protein kinase kinase kinase kinase (MAP4K) family members function in parallel to and are partially redundant with MST1/2 in regulating LATS in response to upstream signals.

    • Zhipeng Meng
    • , Toshiro Moroishi
    •  & Kun-Liang Guan
  • Article
    | Open Access

    Components of the Hippo signalling pathway localize to apical junctions in epithelial cells, where they regulate growth in response to mechanical and biochemical cues. Sun et al. show that these proteins are organized into distinct junctional complexes, which reorganize up on Hippo pathway activation.

    • Shuguo Sun
    • , B. V. V. G. Reddy
    •  & Kenneth D. Irvine
  • Article
    | Open Access

    Essential amino acids such as leucine activate mTORC1 signalling after entering the lysosome, but the molecular basis for lysosomal amino-acid uptake is unclear. Here Milkereit et al. show that LAPTM4b, a lysosomal membrane protein, recruits a leucine transporter to the lysosome and promotes amino-acid influx and mTORC1 signalling.

    • Ruth Milkereit
    • , Avinash Persaud
    •  & Daniela Rotin
  • Article |

    Angiogenesis is regulated by dynamic changes in endothelial cell contact. Here, the authors show that signals from endothelial cell junctions affect the subcellular localization and function of Yes-associated protein, ultimately modifying angiopoietin-2 expression and angiogenic activity of endothelial cells.

    • Hyun-Jung Choi
    • , Haiying Zhang
    •  & Young-Guen Kwon
  • Article |

    Transcriptional regulators Sox2 and YAP maintain expression of stemness genes in normal and cancerous cells. Here the authors show that, in osteosarcomas, Sox2 activates YAP by directly repressing transcription of its upstream negative regulators Nf2 and WWC1, promoting cancer cell stemness.

    • Upal Basu-Roy
    • , N. Sumru Bayin
    •  & Claudio Basilico
  • Article
    | Open Access

    Cells grown on a stiff substrate are stimulated through physical cues to spread, create actin stress fibres and proliferate. Here Cui et al. show that cyclic stretching cells on a soft pillar substrate has the same effect as growth on a stiff substrate, and results in nuclear translocation of YAP and MRTF-A.

    • Yidan Cui
    • , Feroz M. Hameed
    •  & Michael Sheetz
  • Article |

    The Hippo pathway plays a role in regulating organ size and stem cell renewal but the regulatory mechanisms that fine-tune this pathway are not well understood. Here the authors report on the role of NEDD4 as a negative regulator of the Hippo signalling components, WW45 and LATS kinase, and in controlling cell proliferation and intestinal stem cell homeostasis.

    • Sung Jun Bae
    • , Myungjin Kim
    •  & Jae Hong Seol
  • Article |

    The Yes-associated protein (YAP) is a core effector of the Hippo pathway, which regulates proliferation and apoptosis in organ development, but its function in adult skeletal muscle remains poorly defined. Here the authors show that YAP is an essential regulator of myofibre size in adult skeletal muscle, via interaction with TEAD transcription factors.

    • K. I. Watt
    • , B. J. Turner
    •  & P. Gregorevic
  • Article |

    Although much is known about the structural and trafficking molecules involved in generation of primary cilia, the signalling proteins that regulate ciliogenesis are poorly defined. Here, Kim et al. identify the MST1/2-SAV1 complex, a core component of the Hippo pathway, as a key regulator of ciliogenesis in cells and zebrafish.

    • Miju Kim
    • , Minchul Kim
    •  & Dae-Sik Lim
  • Article
    | Open Access

    LAMTOR2 is involved in mTOR and ERK signalling and plays a role in immunity, but its function in dendritic cells (DCs) is not clear. Here the authors show that deletion of LAMTOR2 in DCs results in increased mTOR signalling, accumulation of Flt3 on the cell surface and excessive DC proliferation in ageing mice.

    • Julia M. Scheffler
    • , Florian Sparber
    •  & Lukas A. Huber
  • Article
    | Open Access

    Angiomotins retain the transcription co-activator YAP in the cytoplasm and thereby regulate the Hippo pathway in mammalian cultured cells. Here Leung and Zernicka-Goetz show that Angiomotin family members prevent the differentiation of inner cell mass cells in the mouse blastocyst, via both Hippo pathway-dependent and -independent mechanisms.

    • Chuen Yan Leung
    •  & Magdalena Zernicka-Goetz
  • Article
    | Open Access

    Tumour suppressors can be inactivated in cancer not only as a result of mutation, but also by proteolytic degradation. Here the authors show that, during glioma development, the accumulation of the ubiquitin ligase praja2 sustains tumour growth by degrading MOB1—a core component of the Hippo pathway.

    • Luca Lignitto
    • , Antonietta Arcella
    •  & Antonio Feliciello
  • Article |

    The fusion of satellite cells to muscle fibres during adult life is required for both muscle growth and regeneration but it is unknown whether non-muscle cells contribute to this process. Now, Dellavalle and colleagues show that pericytes, cells associated with the vasculature can contribute to both growth and regeneration of muscle fibres.

    • A. Dellavalle
    • , G. Maroli
    •  & G. Cossu
  • Article
    | Open Access

    What controls the binding partner selection of the target of rapamycin protein, TOR, is unknown. Using theCaenorhabditis elegans tail as a model, Nukazuka et al. determine that signals of semaphorin through plexin control the binding partner selection of TOR and are required for the correct organization of rays in the tail.

    • Akira Nukazuka
    • , Shusaku Tamaki
    •  & Shin Takagi