Cell biology

  • Article
    | Open Access

    ESCRT-III complexes assemble in vivo inside membrane structures with a negative Gaussian curvature, but how membrane shape influences ESCRT-III polymerization remains unclear. Here authors use structural and biophysical methods to show how human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation.

    • Aurélie Bertin
    • , Nicola de Franceschi
    • , Eugenio de la Mora
    • , Sourav Maiti
    • , Maryam Alqabandi
    • , Nolwen Miguet
    • , Aurélie di Cicco
    • , Wouter H. Roos
    • , Stéphanie Mangenot
    • , Winfried Weissenhorn
    •  & Patricia Bassereau
  • Article
    | Open Access

    Oocyte meiosis must achieve spindle bipolarization without predefined spatial cues. Yoshida et al. demonstrate that spindle bipolarization during meiosis I in mouse oocytes requires kinetochores to prevent chromosome segregation errors, a phenomenon that does not occur in error-prone human oocytes.

    • Shuhei Yoshida
    • , Sui Nishiyama
    • , Lisa Lister
    • , Shu Hashimoto
    • , Tappei Mishina
    • , Aurélien Courtois
    • , Hirohisa Kyogoku
    • , Takaya Abe
    • , Aki Shiraishi
    • , Meenakshi Choudhary
    • , Yoshiharu Nakaoka
    • , Mary Herbert
    •  & Tomoya S. Kitajima
  • Article
    | Open Access

    Mammary epithelial cells are epigenetically modified during pregnancy, these changes can influence the pre-disposition to cancer. Here, the authors examine the epigenetic landscape of mammary epithelial cells pre and post pregnancy and identify changes to the epigenetic landscape, which can protect mice from Myc induced cancer.

    • Mary J. Feigman
    • , Matthew A. Moss
    • , Chen Chen
    • , Samantha L. Cyrill
    • , Michael F. Ciccone
    • , Marygrace C. Trousdell
    • , Shih-Ting Yang
    • , Wesley D. Frey
    • , John E. Wilkinson
    •  & Camila O. dos Santos
  • Article
    | Open Access

    The DIS3L2 exonuclease degrades aberrant 7SL RNAs tagged by an oligouridine 3′-tail. Here the authors analyze DIS3L2 knockout mouse embryonic stem cells and suggest that DIS3L2-mediated quality control of 7SL RNA is important for ER-mediated translation and calcium ion homeostasis.

    • Mehdi Pirouz
    • , Chih-Hao Wang
    • , Qi Liu
    • , Aref G. Ebrahimi
    • , Farnaz Shamsi
    • , Yu-Hua Tseng
    •  & Richard I. Gregory
  • Article
    | Open Access

    The ubiquitous oceanic bacteria harbour an external phosphate buffer for modulating phosphate (Pi) uptake. Here, using both oceanic SAR11, Prochlorococcus and Synechococcus strains as a model, the authors show that the Pi buffer accumulation in the periplasm is proton motive force-dependent and can be enhanced by light energy.

    • Nina A. Kamennaya
    • , Kalotina Geraki
    • , David J. Scanlan
    •  & Mikhail V. Zubkov
  • Article
    | Open Access

    Acquired resistance limits the efficacy of PARP inhibitors (PARPi) in high grade serous ovarian cancer (HGSOC). Here, the authors show that inhibition of RNA polymerase I transcription using CX-5461 increases the therapeutic efficacy of PARPi and overcomes PARPi resistance in PDX models of HGSOC.

    • Elaine Sanij
    • , Katherine M. Hannan
    • , Jiachen Xuan
    • , Shunfei Yan
    • , Jessica E. Ahern
    • , Anna S. Trigos
    • , Natalie Brajanovski
    • , Jinbae Son
    • , Keefe T. Chan
    • , Olga Kondrashova
    • , Elizabeth Lieschke
    • , Matthew J. Wakefield
    • , Daniel Frank
    • , Sarah Ellis
    • , Carleen Cullinane
    • , Jian Kang
    • , Gretchen Poortinga
    • , Purba Nag
    • , Andrew J. Deans
    • , Kum Kum Khanna
    • , Linda Mileshkin
    • , Grant A. McArthur
    • , John Soong
    • , Els M. J. J. Berns
    • , Ross D. Hannan
    • , Clare L. Scott
    • , Karen E. Sheppard
    •  & Richard B. Pearson
  • Article
    | Open Access

    The bromodomain containing protein BRD9 has been reported to regulate chromatin remodeling and transcription. Here the authors reveal a role for BRD9 in homologous recombination by facilitating RAD51–RAD54 interaction.

    • Qin Zhou
    • , Jinzhou Huang
    • , Chao Zhang
    • , Fei Zhao
    • , Wootae Kim
    • , Xinyi Tu
    • , Yong Zhang
    • , Somaira Nowsheen
    • , Qian Zhu
    • , Min Deng
    • , Yuping Chen
    • , Bo Qin
    • , Kuntian Luo
    • , Baohua Liu
    • , Zhenkun Lou
    • , Robert W. Mutter
    •  & Jian Yuan
  • Article
    | Open Access

    The nuclear pore complex components, nucleoporins, have been proposed to mediate spatial and temporal organization of chromatin. Here, the authors show that Nucleoporin 153 interacts with CTCF and cohesin, and mediates their binding across cis-regulatory elements and TAD boundaries in mouse embryonic stem cells.

    • Shinichi Kadota
    • , Jianhong Ou
    • , Yuming Shi
    • , Jeannie T. Lee
    • , Jiayu Sun
    •  & Eda Yildirim
  • Article
    | Open Access

    The relationship between histopathology, gene expression, and biochemical and mechanical properties of wounds is largely unknown. Here, the authors show that activin A alters wound healing at multiple levels by promoting pro-fibrotic gene expression and matrix deposition, thereby affecting biomechanical properties of skin wounds.

    • Mateusz S. Wietecha
    • , Marco Pensalfini
    • , Michael Cangkrama
    • , Bettina Müller
    • , Juyoung Jin
    • , Jürgen Brinckmann
    • , Edoardo Mazza
    •  & Sabine Werner
  • Article
    | Open Access

    Mitochondrial dynamics change during ageing, with larger mitochondria and altered protein import in older animals. Here the authors show that Dosmit protein mediates mitochondrial morphology with Rab32 by inducing double-membraned vesicles that regulate protein trafficking into mitochondria.

    • Po-Lin Chen
    • , Kai-Ting Huang
    • , Chu-Ya Cheng
    • , Jian-Chiuan Li
    • , Hsiao-Yen Chan
    • , Tzu-Yang Lin
    • , Matthew P. Su
    • , Wei-Yuan Yang
    • , Henry C. Chang
    • , Horng-Dar Wang
    •  & Chun-Hong Chen
  • Article
    | Open Access

    The cytokine IFNα is expressed in the islets of individuals with type 1 diabetes and contributes to local inflammation and destruction of beta cells. Here, the authors provide a global multiomics view of IFNα-induced changes in human beta cells at the level of chromatin, mRNA and protein expression.

    • Maikel L. Colli
    • , Mireia Ramos-Rodríguez
    • , Ernesto S. Nakayasu
    • , Maria I. Alvelos
    • , Miguel Lopes
    • , Jessica L. E. Hill
    • , Jean-Valery Turatsinze
    • , Alexandra Coomans de Brachène
    • , Mark A. Russell
    • , Helena Raurell-Vila
    • , Angela Castela
    • , Jonàs Juan-Mateu
    • , Bobbie-Jo M. Webb-Robertson
    • , Lars Krogvold
    • , Knut Dahl-Jorgensen
    • , Lorella Marselli
    • , Piero Marchetti
    • , Sarah J. Richardson
    • , Noel G. Morgan
    • , Thomas O. Metz
    • , Lorenzo Pasquali
    •  & Décio L. Eizirik
  • Article
    | Open Access

    The molecular mechanisms that regulate intestinal Clu+ revival stem cells (revSCs) and their niche to enable regeneration in response to injury are unclear. Here, the authors show that mice without the phospholipid transport protein, TNFAIP8, causes less revSCs to be induced following injury.

    • Jason R. Goldsmith
    • , Nina Spitofsky
    • , Ali Zamani
    • , Ryan Hood
    • , Amanda Boggs
    • , Xinyuan Li
    • , Mingyue Li
    • , Elizabeth Reiner
    • , Arshad Ayyaz
    • , Zienab Etwebi
    • , Ling Lu
    • , Javier Rivera Guzman
    • , Mayassa J. Bou-Dargham
    • , Terry Cathoupolis
    • , Hakon Hakonarson
    • , Honghong Sun
    • , Jeffrey L. Wrana
    • , Michael V. Gonzalez
    •  & Youhai H. Chen
  • Article
    | Open Access

    During the progression of type 2 diabetes, insulin-producing β-cells can lose their identity and become reprogrammed into other cell types. Here the authors show that murine diabetic β-cells require the protein Raptor for maintaining β-cell health and preventing them from turning into α-cells, independent of Raptor’s involvement in regulating blood sugar levels.

    • Qinglei Yin
    • , Qicheng Ni
    • , Yichen Wang
    • , Hongli Zhang
    • , Wenyi Li
    • , Aifang Nie
    • , Shu Wang
    • , Yanyun Gu
    • , Qidi Wang
    •  & Guang Ning
  • Article
    | Open Access

    Chronic lung diseases are characterized by molecular and cellular composition changes. Here the authors use single-cell RNA sequencing to map cell type-specific changes in human tracheal epithelium related to smoking, and to provide evidence for a tuft-like progenitor for pulmonary neuroendocrine cells and ionocytes.

    • Katherine C. Goldfarbmuren
    • , Nathan D. Jackson
    • , Satria P. Sajuthi
    • , Nathan Dyjack
    • , Katie S. Li
    • , Cydney L. Rios
    • , Elizabeth G. Plender
    • , Michael T. Montgomery
    • , Jamie L. Everman
    • , Preston E. Bratcher
    • , Eszter K. Vladar
    •  & Max A. Seibold
  • Article
    | Open Access

    Protein synthesis suppression protects breast cancer cells from clinically relevant stresses like hypoxia. Here, the authors show that unique mRNA isoforms that govern stem cell-like phenotypes escape translational repression to drive tumor progression and chemoresistance.

    • Michael Jewer
    • , Laura Lee
    • , Matthew Leibovitch
    • , Guihua Zhang
    • , Jiahui Liu
    • , Scott D. Findlay
    • , Krista M. Vincent
    • , Kristofferson Tandoc
    • , Dylan Dieters-Castator
    • , Daniela F. Quail
    • , Indrani Dutta
    • , Mackenzie Coatham
    • , Zhihua Xu
    • , Aakshi Puri
    • , Bo-Jhih Guan
    • , Maria Hatzoglou
    • , Andrea Brumwell
    • , James Uniacke
    • , Christos Patsis
    • , Antonis Koromilas
    • , Julia Schueler
    • , Gabrielle M. Siegers
    • , Ivan Topisirovic
    •  & Lynne-Marie Postovit
  • Article
    | Open Access

    Fast metabolisms tend to shorten lifespans by increasing oxidative damage. This study identifies a gene mutation that keeps a key antioxidant response active, possibly allowing Neoaves bird species to avoid the tradeoff between rapid metabolism and longevity that challenges most mammals, including humans.

    • Gianni M. Castiglione
    • , Zhenhua Xu
    • , Lingli Zhou
    •  & Elia J. Duh
  • Article
    | Open Access

    The α-cardiac actin M305L hypertrophic cardiomyopathy-causing mutation is located near residues that help confine tropomyosin to an inhibitory position along thin filaments. Here the authors assessed M305L actin in vivo, in vitro, and in silico to characterize emergent pathological properties and define the mechanistic basis of disease.

    • Meera C. Viswanathan
    • , William Schmidt
    • , Peter Franz
    • , Michael J. Rynkiewicz
    • , Christopher S. Newhard
    • , Aditi Madan
    • , William Lehman
    • , Douglas M. Swank
    • , Matthias Preller
    •  & Anthony Cammarato
  • Article
    | Open Access

    The development of chemoresistance is a major hurdle in triple negative breast cancer (TNBC). Here, the authors show that lysyl oxidase (LOX) is overexpressed in chemoresistant TNBCs, and when inhibited reduces collagen cross-linking, fibronectin fibril assembly, and downstream integrin signalling, overcoming resistance.

    • Ozge Saatci
    • , Aysegul Kaymak
    • , Umar Raza
    • , Pelin G. Ersan
    • , Ozge Akbulut
    • , Carolyn E. Banister
    • , Vitali Sikirzhytski
    • , Unal Metin Tokat
    • , Gamze Aykut
    • , Suhail A. Ansari
    • , Hayriye Tatli Dogan
    • , Mehmet Dogan
    • , Pouria Jandaghi
    • , Aynur Isik
    • , Fatma Gundogdu
    • , Kemal Kosemehmetoglu
    • , Omer Dizdar
    • , Sercan Aksoy
    • , Aytekin Akyol
    • , Aysegul Uner
    • , Phillip J. Buckhaults
    • , Yasser Riazalhosseini
    •  & Ozgur Sahin
  • Article
    | Open Access

    The essential role of ORAI1 channels in receptor-evoked Ca2+ signaling is well understood, but the roles of ORAI2 and ORAI3 remained obscure. Here authors show that ORAI2 and ORAI3 channels multimerize with ORAI1 to expand the range of sensitivity of receptor-activated Ca2+ signals, reflecting their enhanced basal STIM1-binding and heightened Ca2+-dependent inactivation.

    • Ryan E. Yoast
    • , Scott M. Emrich
    • , Xuexin Zhang
    • , Ping Xin
    • , Martin T. Johnson
    • , Adam J. Fike
    • , Vonn Walter
    • , Nadine Hempel
    • , David I. Yule
    • , James Sneyd
    • , Donald L. Gill
    •  & Mohamed Trebak
  • Article
    | Open Access

    IRE1α plays a key role in the unfolded protein response (UPR) by promoting the unconventional splicing of the XBP1 and the selective cleavage of RNAs. Here the authors report that IRE1α is activated upon the DNA damage response and selectively controls the stability of mRNAs to maintain genome integrity.

    • Estefanie Dufey
    • , José Manuel Bravo-San Pedro
    • , Cristian Eggers
    • , Matías González-Quiroz
    • , Hery Urra
    • , Alfredo I. Sagredo
    • , Denisse Sepulveda
    • , Philippe Pihán
    • , Amado Carreras-Sureda
    • , Younis Hazari
    • , Eduardo A. Sagredo
    • , Daniela Gutierrez
    • , Cristian Valls
    • , Alexandra Papaioannou
    • , Diego Acosta-Alvear
    • , Gisela Campos
    • , Pedro M. Domingos
    • , Rémy Pedeux
    • , Eric Chevet
    • , Alejandra Alvarez
    • , Patricio Godoy
    • , Peter Walter
    • , Alvaro Glavic
    • , Guido Kroemer
    •  & Claudio Hetz
  • Article
    | Open Access

    Protein arginine methyltransferases (PRMTs) are increasingly recognized as potential therapeutic targets but PRMT7 remains an understudied member of this enzyme family. Here, the authors develop a chemical probe for PRMT7 and apply it to elucidate the role of PRMT7 in the cellular stress response.

    • Magdalena M. Szewczyk
    • , Yoshinori Ishikawa
    • , Shawna Organ
    • , Nozomu Sakai
    • , Fengling Li
    • , Levon Halabelian
    • , Suzanne Ackloo
    • , Amber L. Couzens
    • , Mohammad Eram
    • , David Dilworth
    • , Hideto Fukushi
    • , Rachel Harding
    • , Carlo C. dela Seña
    • , Tsukasa Sugo
    • , Kozo Hayashi
    • , David McLeod
    • , Carlos Zepeda
    • , Ahmed Aman
    • , Maria Sánchez-Osuna
    • , Eric Bonneil
    • , Shinji Takagi
    • , Rima Al-Awar
    • , Mike Tyers
    • , Stephane Richard
    • , Masayuki Takizawa
    • , Anne-Claude Gingras
    • , Cheryl H. Arrowsmith
    • , Masoud Vedadi
    • , Peter J. Brown
    • , Hiroshi Nara
    •  & Dalia Barsyte-Lovejoy
  • Article
    | Open Access

    YAP1 gene fusions are found in subgroups of paediatric ependymomas. Here the authors show that YAP1 activation in NeuroD6 positive neuronal precursor cells can induce ependymoma-like tumours in mice.

    • Noreen Eder
    • , Federico Roncaroli
    • , Marie-Charlotte Dolmart
    • , Stuart Horswell
    • , Felipe Andreiuolo
    • , Helen R. Flynn
    • , Andre T. Lopes
    • , Suzanne Claxton
    • , John-Paul Kilday
    • , Lucy Collinson
    • , Jun-Hao Mao
    • , Torsten Pietsch
    • , Barry Thompson
    • , Ambrosius P. Snijders
    •  & Sila K. Ultanir
  • Article
    | Open Access

    Import of proteins into specific cellular compartments is critical for organelle function and several proteins are known to be imported into multiple compartments. Here, the authors report that the protein Ptc5 is first sorted to and processed in the mitochondria before being targeted to peroxisomes, which may influence mitochondria-peroxisome interorganellar contact.

    • Thorsten Stehlik
    • , Marco Kremp
    • , Jörg Kahnt
    • , Michael Bölker
    •  & Johannes Freitag
  • Article
    | Open Access

    Naked mole rats are the longest-lived rodents and produce very-high-molecular-mass hyaluronan (vHMM-HA). Here the authors show that naked mole rat vHMM-HA is better at protecting mouse and human cells from cell cycle arrest and cell death, compared to the high-molecular-mass hyaluronan produced by these species.

    • Masaki Takasugi
    • , Denis Firsanov
    • , Gregory Tombline
    • , Hanbing Ning
    • , Julia Ablaeva
    • , Andrei Seluanov
    •  & Vera Gorbunova
  • Article
    | Open Access

    Progressive fibrosis is a driver of morbidity and mortality in many chronic liver diseases, but the underlying mechanisms are incompletely understood. Here, the authors show that mitochondria-derived damage-associated molecular patterns are released from injured hepatocytes and can trigger fibrogenic activation of hepatic stellate cells.

    • Ping An
    • , Lin-Lin Wei
    • , Shuangshuang Zhao
    • , Deanna Y. Sverdlov
    • , Kahini A. Vaid
    • , Makoto Miyamoto
    • , Kaori Kuramitsu
    • , Michelle Lai
    •  & Yury V. Popov
  • Article
    | Open Access

    The downstream pathway regulating how TGF-beta affects pluripotency of human PSCs is unclear. Here, the authors find that transcription factor ZNF398 binds active promoters/enhancers together with the histone acetyltransferase EP300 and SMAD3, enabling expression of pluripotency and epithelial genes.

    • Irene Zorzan
    • , Marco Pellegrini
    • , Mattia Arboit
    • , Danny Incarnato
    • , Mara Maldotti
    • , Mattia Forcato
    • , Guidantonio Malagoli Tagliazucchi
    • , Elena Carbognin
    • , Marco Montagner
    • , Salvatore Oliviero
    •  & Graziano Martello
  • Article
    | Open Access

    The evolutionarily conserved complex, the Par proteins, regulates cell polarity. Here, the authors show that in Drosophila neuroblasts, the Par complex exhibits liquid–liquid phase separation dependent on the cell cycle.

    • Ziheng Liu
    • , Ying Yang
    • , Aihong Gu
    • , Jiawen Xu
    • , Ying Mao
    • , Haojie Lu
    • , Weiguo Hu
    • , Qun-Ying Lei
    • , Zhouhua Li
    • , Mingjie Zhang
    • , Yu Cai
    •  & Wenyu Wen
  • Article
    | Open Access

    In budding yeast, the decision to enter meiosis is defined by nutrient and mating-type signals regulating expression of the master transcription factor for meiotic entry, IME1. Here, the authors characterize the steps involved in regulating the entering in meiosis via the co-repressor complex Tup1-Cyc8.

    • Janis Tam
    •  & Folkert J. van Werven
  • Article
    | Open Access

    In the S phase of the cell cycle, the full genome needs to be replicated before cell division occurs. Here, authors show that in budding yeast DNA synthesis is completed after chromosome segregation begins.

    • Tsvetomira Ivanova
    • , Michael Maier
    • , Alsu Missarova
    • , Céline Ziegler-Birling
    • , Monica Dam
    • , Mercè Gomar-Alba
    • , Lucas B. Carey
    •  & Manuel Mendoza
  • Article
    | Open Access

    Inflammasome activation is a response to bacterial infection but can cause damage and spread infection. Here, the authors use live single-cell imaging to show two mechanisms by which M. tuberculosis causes damage to human macrophage cell plasma membranes, resulting in activation of the NLRP3 inflammasome, pyroptosis and release of infectious particles.

    • Kai S. Beckwith
    • , Marianne S. Beckwith
    • , Sindre Ullmann
    • , Ragnhild S. Sætra
    • , Haelin Kim
    • , Anne Marstad
    • , Signe E. Åsberg
    • , Trine A. Strand
    • , Markus Haug
    • , Michael Niederweis
    • , Harald A. Stenmark
    •  & Trude H. Flo
  • Article
    | Open Access

    p85β (PIK3R2), a regulatory subunit of PI3K, has oncogenic properties. Here the authors show that p85β promotes AXL protein stability, which in turn activates p110 to induce PDK1/SGK3 signaling, and therapeutically, p85β-expressing ovarian cancer cells are sensitive to AXL inhibition.

    • Ling Rao
    • , Victor C. Y. Mak
    • , Yuan Zhou
    • , Dong Zhang
    • , Xinran Li
    • , Chloe C. Y. Fung
    • , Rakesh Sharma
    • , Chao Gu
    • , Yiling Lu
    • , George L. Tipoe
    • , Annie N. Y. Cheung
    • , Gordon B. Mills
    •  & Lydia W. T. Cheung
  • Article
    | Open Access

    Macrophages undergo a Warburg-like switch from oxidative phosphorylation to glycolysis in response to inflammatory stimulus. Here the authors show that fungal melanin can trigger this switch in human macrophages by sequestering calcium in the phagosome and enabling protection against Aspergillus fumigatus infection.

    • Samuel M. Gonçalves
    • , Cláudio Duarte-Oliveira
    • , Cláudia F. Campos
    • , Vishukumar Aimanianda
    • , Rob ter Horst
    • , Luis Leite
    • , Toine Mercier
    • , Paulo Pereira
    • , Miguel Fernández-García
    • , Daniela Antunes
    • , Cláudia S. Rodrigues
    • , Catarina Barbosa-Matos
    • , Joana Gaifem
    • , Inês Mesquita
    • , António Marques
    • , Nuno S. Osório
    • , Egídio Torrado
    • , Fernando Rodrigues
    • , Sandra Costa
    • , Leo AB. Joosten
    • , Katrien Lagrou
    • , Johan Maertens
    • , João F. Lacerda
    • , António Campos Jr.
    • , Gordon D. Brown
    • , Axel A. Brakhage
    • , Coral Barbas
    • , Ricardo Silvestre
    • , Frank L. van de Veerdonk
    • , Georgios Chamilos
    • , Mihai G. Netea
    • , Jean-Paul Latgé
    • , Cristina Cunha
    •  & Agostinho Carvalho
  • Article
    | Open Access

    Proteins KaiA, KaiB and KaiC constitute a biochemical circadian oscillator in Synechococcus cyanobacteria. Here, Kawamoto et al. show that kaiBC promoter activity exhibits a damped, low-amplitude circadian oscillation in the absence of KaiA, which could explain the circadian rhythms observed in other bacteria that lack a kaiA homologue.

    • Naohiro Kawamoto
    • , Hiroshi Ito
    • , Isao T. Tokuda
    •  & Hideo Iwasaki
  • Article
    | Open Access

    Cell competition among epithelial cells allows removal of unfit or dangerous cells. Here, the authors show that the NMDA receptor is an important determinant of cell fitness in the Drosophila wing, also in the context of Myc super-competitor cells, with “loser” cells contributing metabolitic fuel to “winner” cells.

    • Agnes R. Banreti
    •  & Pascal Meier
  • Article
    | Open Access

    In macrophages, IL-1β secretion is mediated by N-GSDMD pores in the plasma membrane (PM). Here the authors show that in neutrophils, IL-1β secretion occurs in the absence of PM pores, via autophagosomes; N-GSDMD does not traffic to PM but to azurophilic granules, thereby releasing neutrophil elastase which cleaves further N-GSDMD into alternative fragments.

    • Mausita Karmakar
    • , Martin Minns
    • , Elyse N. Greenberg
    • , Jose Diaz-Aponte
    • , Kersi Pestonjamasp
    • , Jennifer L. Johnson
    • , Joseph K. Rathkey
    • , Derek W. Abbott
    • , Kun Wang
    • , Feng Shao
    • , Sergio D. Catz
    • , George R. Dubyak
    •  & Eric Pearlman
  • Article
    | Open Access

    The function on subgenomic flaviviral RNA (sfRNA) in the mosquito vector is not well understood. Here, Slonchak et al. show that sfRNA affects virus-induced apoptosis and dissemination of ZIKV in Aedes aegypti mosquitoes, suggesting a role of sfRNA in Zika virus replication and transmission.

    • Andrii Slonchak
    • , Leon E. Hugo
    • , Morgan E. Freney
    • , Sonja Hall-Mendelin
    • , Alberto A. Amarilla
    • , Francisco J. Torres
    • , Yin Xiang Setoh
    • , Nias Y. G. Peng
    • , Julian D. J. Sng
    • , Roy A. Hall
    • , Andrew F. van den Hurk
    • , Gregor J. Devine
    •  & Alexander A. Khromykh
  • Article
    | Open Access

    Molecular mechanisms linking myocardial insulin resistance to diabetic cardiomyopathy are incompletely understood. Here the authors show that myocardial insulin resistance impairs a PKB-SPEG-SERCA2a signaling axis, which contributes to the development of diabetic cardiomyopathy.

    • Chao Quan
    • , Qian Du
    • , Min Li
    • , Ruizhen Wang
    • , Qian Ouyang
    • , Shu Su
    • , Sangsang Zhu
    • , Qiaoli Chen
    • , Yang Sheng
    • , Liang Chen
    • , Hong Wang
    • , David G. Campbell
    • , Carol MacKintosh
    • , Zhongzhou Yang
    • , Kunfu Ouyang
    • , Hong Yu Wang
    •  & Shuai Chen
  • Article
    | Open Access

    Most cells possess sensory cilia, which need to be gated properly. Here the authors show that the C. elegans proteins TALP-3 and ANKR-26 coordinate cilia gating in the context of transition fibers and that this mechanism is conserved in mammalian cells and likely implicated in certain ciliopathies.

    • Hao Yan
    • , Chuan Chen
    • , Huicheng Chen
    • , Hui Hong
    • , Yan Huang
    • , Kun Ling
    • , Jinghua Hu
    •  & Qing Wei
  • Article
    | Open Access

    The contribution of central and peripheral channels of nuclear pores to transport of transmembrane proteins is unclear. Here the authors show that most inner nuclear membrane proteins use only peripheral channels, but some extend nuclear localization signals into the central channel for directed nuclear transport.

    • Krishna C. Mudumbi
    • , Rafal Czapiewski
    • , Andrew Ruba
    • , Samuel L. Junod
    • , Yichen Li
    • , Wangxi Luo
    • , Christina Ngo
    • , Valentina Ospina
    • , Eric C. Schirmer
    •  & Weidong Yang
  • Article
    | Open Access

    The physiological role of the olfactory marker protein (OMP) has been elusive. Here, the authors demonstrate that OMP buffers cAMP and modulates cAMP-gated channel activity upon sensory stimulation, maintaining neuronal firing during odour-source searching.

    • Noriyuki Nakashima
    • , Kie Nakashima
    • , Akiko Taura
    • , Akiko Takaku-Nakashima
    • , Harunori Ohmori
    •  & Makoto Takano
  • Article
    | Open Access

    During cell migration, cells are polarized with distinct front vs. rear regions but whether and how polarity is transmitted to the nucleus is unclear. Here the authors show that frontally-biased endoplasmic reticulum and the nuclear membrane protein Emerin contribute to front-rear nuclear cell polarity.

    • Paulina Nastały
    • , Divya Purushothaman
    • , Stefano Marchesi
    • , Alessandro Poli
    • , Tobias Lendenmann
    • , Gururaj Rao Kidiyoor
    • , Galina V. Beznoussenko
    • , Stefania Lavore
    • , Orso Maria Romano
    • , Dimos Poulikakos
    • , Marco Cosentino Lagomarsino
    • , Alexander A. Mironov
    • , Aldo Ferrari
    •  & Paolo Maiuri
  • Article
    | Open Access

    Calvo-Rodriguez et al. show elevated calcium levels in neuronal mitochondria in a mouse model of cerebral β-amyloidosis after plaque deposition, which precede rare neuron death events in this model. The mechanism involves toxic extracellular Aβ oligomers and the mitochondrial calcium uniporter.

    • Maria Calvo-Rodriguez
    • , Steven S. Hou
    • , Austin C. Snyder
    • , Elizabeth K. Kharitonova
    • , Alyssa N. Russ
    • , Sudeshna Das
    • , Zhanyun Fan
    • , Alona Muzikansky
    • , Monica Garcia-Alloza
    • , Alberto Serrano-Pozo
    • , Eloise Hudry
    •  & Brian J. Bacskai
  • Article
    | Open Access

    The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization.

    • Paweł Trzaskoma
    • , Błażej Ruszczycki
    • , Byoungkoo Lee
    • , Katarzyna K. Pels
    • , Katarzyna Krawczyk
    • , Grzegorz Bokota
    • , Andrzej A. Szczepankiewicz
    • , Jesse Aaron
    • , Agnieszka Walczak
    • , Małgorzata A. Śliwińska
    • , Adriana Magalska
    • , Michal Kadlof
    • , Artur Wolny
    • , Zofia Parteka
    • , Sebastian Arabasz
    • , Magdalena Kiss-Arabasz
    • , Dariusz Plewczyński
    • , Yijun Ruan
    •  & Grzegorz M. Wilczyński
  • Article
    | Open Access

    PCNA is essential for DNA replication and cellular proliferation. Here, the authors reveal that PCNA ubiquitination protects stalled replication forks from DNA2-mediated degradation via regulation of Okazaki fragment maturation and chromatin assembly.

    • Tanay Thakar
    • , Wendy Leung
    • , Claudia M. Nicolae
    • , Kristen E. Clements
    • , Binghui Shen
    • , Anja-Katrin Bielinsky
    •  & George-Lucian Moldovan
  • Article
    | Open Access

    The origin of the heterogeneity of metabolic and inflammatory profiles exhibited by white adipocytes is little understood. Here, using scRNA-seq and computational methods, the authors show that differentiating preadipocytes exhibit gene expression differences and suggest underlying regulators.

    • Alfred K. Ramirez
    • , Simon N. Dankel
    • , Bashir Rastegarpanah
    • , Weikang Cai
    • , Ruidan Xue
    • , Mark Crovella
    • , Yu-Hua Tseng
    • , C. Ronald Kahn
    •  & Simon Kasif
  • Article
    | Open Access

    A prior live-cell exosome reporter showed dim fluorescence and could not be expressed stably, limiting its usefulness. Here the authors stabilise the reporter to allow long-term tracking of exosomes, and incorporate a second fluorophore to visualise the entire exosome lifecycle.

    • Bong Hwan Sung
    • , Ariana von Lersner
    • , Jorge Guerrero
    • , Evan S. Krystofiak
    • , David Inman
    • , Roxanne Pelletier
    • , Andries Zijlstra
    • , Suzanne M. Ponik
    •  & Alissa M. Weaver
  • Article
    | Open Access

    Hyper-insulinemia associated with excess calorie intake may cause metabolic dysfunction. Here the authors report that mice with partially reduced insulin receptor expression in peripheral tissues are protected from and experience reversal of fatty liver disease.

    • Troy L. Merry
    • , Chris P. Hedges
    • , Stewart W. Masson
    • , Beate Laube
    • , Doris Pöhlmann
    • , Stephan Wueest
    • , Michael E. Walsh
    • , Myrtha Arnold
    • , Wolfgang Langhans
    • , Daniel Konrad
    • , Kim Zarse
    •  & Michael Ristow
  • Article
    | Open Access

    Codon 158 gain-of-function mutant p53 (158-mutp53) promotes tumourigenesis in lung cancer. Here, the authors show that 158-mutp53 render cancers sensitive to cisplatin and p53 acetylation agents through a mechanism where acetylated mutant p53 upregulates TRAIP and inhibits NF-ĸB signaling.

    • Li Ren Kong
    • , Richard Weijie Ong
    • , Tuan Zea Tan
    • , Nur Afiqah Binte Mohamed Salleh
    • , Matan Thangavelu
    • , Jane Vin Chan
    • , Lie Yong Judice Koh
    • , Giridharan Periyasamy
    • , Jieying Amelia Lau
    • , Thi Bich Uyen Le
    • , Lingzhi Wang
    • , Miyoung Lee
    • , Srinivasaraghavan Kannan
    • , Chandra S. Verma
    • , Chwee Ming Lim
    • , Wee Joo Chng
    • , David P. Lane
    • , Ashok Venkitaraman
    • , Huynh The Hung
    • , Chit Fang Cheok
    •  & Boon Cher Goh