Biotechnology articles from across Nature Portfolio

Transcription factors containing disordered regions can now be mapped across the genome, aiding functional studies.

Nanopore sequencing combined with DNA-barcoded probes enables multiplexed detection of diverse biomarkers in blood serum.

By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.

Brain injuries can result in significant damage to the cerebral cortex, and restoring the cellular architecture of the tissue remains challenging. Here, the authors use a droplet printing technique to fabricate a simplified human cerebral cortical column and demonstrate its functionality and potential for future personalized therapy approaches.

Microbial production of succinic acid at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation. Here, the authors report an end-to-end pipeline for succinic acid production at low pH using engineered acid-tolerant Issatchenkia orientalis strain.

The emerging field of genetically targeted chemical assembly (GTCA) uses cell-specific genetic information to instruct chemical synthesis in situ. This Perspective discusses recent progress in GTCA and outlines opportunities for expanding the GTCA toolbox and diversifying applications.

Histological assessment of nonalcoholic fatty liver disease (NAFLD) is essential for clinical practice as it outlines diagnosis and lays the foundation of medical care. This Perspective summarizes the advancements in digital histology and discusses current and future applications in NAFLD.

Spectral flow cytometry enables the simultaneous analysis of a large number of cell surface markers at the single-cell level

Point-of-care (POC) diagnostics are particularly important in resource-limited settings. To ensure their sustainability, deployment and uptake by underserved communities, systemic, infrastructural, operational and logistical limitations need to be addressed.

Transcription factors containing disordered regions can now be mapped across the genome, aiding functional studies.

In this Tool of the Trade article, Nicolas Mathey-Andrews describes the generation and use of a prime editor mouse that enables in vivo modelling of the multitude of cancer alleles found in human tumours.

We propose the use of the terms natural gene drive (NGD) and engineered gene drive (EGD) arguing against James et al.¹, who think both should be included within the term “gene drive”, based on their mechanistic similarities.

Guhlin et al. sequenced the genomes of almost the entire extant population of the critically-endangered kākāpō, revealing genetic variants for fitness-related traits that can inform conservation strategies.