Biotechnology articles from across Nature Portfolio

Two major advances in optical pooled screening improve substantially on sensitivity and robustness, expanding its applicability to a broader range of biological contexts.

Sample, financial and labor requirements are key barriers to scaling up high-content phenotypic discovery efforts. A broadly applicable method overcomes these challenges through experimental compression (by pooling various perturbations) and computational deconvolution (of their individual effects), empowering the use of phenotypic screening to advance therapeutic discovery.

A CRISPR-based assay both recognizes and amplifies target mRNA, achieving sub-attomolar sensitivity with single-nucleotide resolution. This method enables the detection of low-abundance mRNA in extracellular vesicles, providing clinically relevant information for precision oncology.

As synthetic biology expands, creating “drag-and-drop” regulatory tools that can achieve diverse regulatory outcomes are paramount. In this work, the Carbon Storage Regulatory Network of Escherichia coli is rewired to create synthetic post-transcriptional genetic circuits that leverage the native interactions and are composed to achieve complex bacterial computation.

Combining expansion microscopy with super-resolution radial fluctuations captures the morphology of single proteins.

Digital twins provide a framework to advance the field of personalized medicine by generating clinically actionable strategies that leverage individualized data as well as current and emerging research. Strong interdisciplinary teamwork, specific funding mechanisms and integration of key biological details such as somatic evolution are necessary for the effective adoption of digital twins in medicine.

Two major advances in optical pooled screening improve substantially on sensitivity and robustness, expanding its applicability to a broader range of biological contexts.

Sample, financial and labor requirements are key barriers to scaling up high-content phenotypic discovery efforts. A broadly applicable method overcomes these challenges through experimental compression (by pooling various perturbations) and computational deconvolution (of their individual effects), empowering the use of phenotypic screening to advance therapeutic discovery.

A CRISPR-based assay both recognizes and amplifies target mRNA, achieving sub-attomolar sensitivity with single-nucleotide resolution. This method enables the detection of low-abundance mRNA in extracellular vesicles, providing clinically relevant information for precision oncology.