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Biotechnology is a broad discipline in which biological processes, organisms, cells or cellular components are exploited to develop new technologies. New tools and products developed by biotechnologists are useful in research, agriculture, industry and the clinic.
By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.
Although base and prime editors can be highly efficient in human hematopoietic stem cells, we find they can cause adverse cellular responses, including reduced engraftment and the generation of DNA double-strand breaks and genotoxic byproducts, albeit at a lower frequency than Cas9. We also find that base editors increase the genome-wide mutagenic load.
A proof of principle approach redirects an anti-IL-1b antibody to bind the otherwise unrelated antigen, IL-17A, highlighting the plasticity of antibody scaffolds that could be manipulated for alternative binding or function.
In this Tool of the Trade article, Nicolas Mathey-Andrews describes the generation and use of a prime editor mouse that enables in vivo modelling of the multitude of cancer alleles found in human tumours.
We propose the use of the terms natural gene drive (NGD) and engineered gene drive (EGD) arguing against James et al.1, who think both should be included within the term “gene drive”, based on their mechanistic similarities.
Guhlin et al. sequenced the genomes of almost the entire extant population of the critically-endangered kākāpō, revealing genetic variants for fitness-related traits that can inform conservation strategies.
