Biotechnology

  • Article
    | Open Access

    Comprehensive epigenomic maps of various rice varieties are still unavailable. Here, the authors report the development of eChIP as a fast and low-input upgrade of regular plant ChIP-seq protocol for epigenome analysis of 20 rice varieties and annotate over 80% of the genome with different epigenome properties for transcriptional regulation.

    • Lun Zhao
    • , Liang Xie
    • , Qing Zhang
    • , Weizhi Ouyang
    • , Li Deng
    • , Pengpeng Guan
    • , Meng Ma
    • , Yue Li
    • , Ying Zhang
    • , Qin Xiao
    • , Jingwen Zhang
    • , Hongmeijuan Li
    • , Shunyao Wang
    • , Jiangwei Man
    • , Zhilin Cao
    • , Qinghua Zhang
    • , Qifa Zhang
    • , Guoliang Li
    •  & Xingwang Li
  • Article
    | Open Access

    Increasing grain yield needs to be put in the context of environmental stress. Here, the authors reveal that a UDP-glucosyltransferase is associated with regulation of rice grain size, abiotic stress tolerance, flavonoid-mediated auxin signaling, and redirection of carbon flux to flavonoid glycosides synthesis.

    • Nai-Qian Dong
    • , Yuwei Sun
    • , Tao Guo
    • , Chuan-Lin Shi
    • , Yi-Min Zhang
    • , Yi Kan
    • , You-Huang Xiang
    • , Hai Zhang
    • , Yi-Bing Yang
    • , Ya-Chao Li
    • , Huai-Yu Zhao
    • , Hong-Xiao Yu
    • , Zi-Qi Lu
    • , Yong Wang
    • , Wang-Wei Ye
    • , Jun-Xiang Shan
    •  & Hong-Xuan Lin
  • Article
    | Open Access

    Ultrasensitive methods for detection of biomarkers for infectious disease are needed for diagnosing, monitoring and targeting treatment. Here the authors develop a digital assay for inflammatory markers, bacterial DNA and antibotic-resistance genes and apply it to characterise asthma patients and predict mortality from septic shock.

    • M. Fatih Abasıyanık
    • , Krysta Wolfe
    • , Hoang Van Phan
    • , Jing Lin
    • , Bharathi Laxman
    • , Steven R. White
    • , Philip A. Verhoef
    • , Gökhan M. Mutlu
    • , Bhakti Patel
    •  & Savaş Tay
  • Article
    | Open Access

    Memory B cells are important for protecting the host from pathogen rechallenge, but their properties and locations remain ill-defined. Here the authors show, using single-cell transcriptomics and repertoire analyses, that mouse spleen and bone marrow host distinct populations of isotype-switched memory B cells to potentially optimize for rapid recall responses.

    • René Riedel
    • , Richard Addo
    • , Marta Ferreira-Gomes
    • , Gitta Anne Heinz
    • , Frederik Heinrich
    • , Jannis Kummer
    • , Victor Greiff
    • , Daniel Schulz
    • , Cora Klaeden
    • , Rebecca Cornelis
    • , Ulrike Menzel
    • , Stefan Kröger
    • , Ulrik Stervbo
    • , Ralf Köhler
    • , Claudia Haftmann
    • , Silvia Kühnel
    • , Katrin Lehmann
    • , Patrick Maschmeyer
    • , Mairi McGrath
    • , Sandra Naundorf
    • , Stefanie Hahne
    • , Özen Sercan-Alp
    • , Francesco Siracusa
    • , Jonathan Stefanowski
    • , Melanie Weber
    • , Kerstin Westendorf
    • , Jakob Zimmermann
    • , Anja E. Hauser
    • , Sai T. Reddy
    • , Pawel Durek
    • , Hyun-Dong Chang
    • , Mir-Farzin Mashreghi
    •  & Andreas Radbruch
  • Article
    | Open Access

    Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protocol.

    • Haitao Chen
    • , Yan Zeng
    • , Yongzhi Yang
    • , Lingli Huang
    • , Bolin Tang
    • , He Zhang
    • , Fei Hao
    • , Wei Liu
    • , Youhan Li
    • , Yanbin Liu
    • , Xiaoshuang Zhang
    • , Ru Zhang
    • , Yesheng Zhang
    • , Yongxin Li
    • , Kun Wang
    • , Hua He
    • , Zhongkai Wang
    • , Guangyi Fan
    • , Hui Yang
    • , Aike Bao
    • , Zhanhuan Shang
    • , Jianghua Chen
    • , Wen Wang
    •  & Qiang Qiu
  • Article
    | Open Access

    Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lowers mutation rates.

    • Ewart Kuijk
    • , Myrthe Jager
    • , Bastiaan van der Roest
    • , Mauro D. Locati
    • , Arne Van Hoeck
    • , Jerome Korzelius
    • , Roel Janssen
    • , Nicolle Besselink
    • , Sander Boymans
    • , Ruben van Boxtel
    •  & Edwin Cuppen
  • Article
    | Open Access

    Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$-NAAN-3$$^{\prime}$$ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.

    • Pranam Chatterjee
    • , Jooyoung Lee
    • , Lisa Nip
    • , Sabrina R. T. Koseki
    • , Emma Tysinger
    • , Erik J. Sontheimer
    • , Joseph M. Jacobson
    •  & Noah Jakimo
  • Review Article
    | Open Access

    Our efforts to build complex synthetic biology circuits are impeded by limited knowledge of optimal combinations. In this review, the authors consider current combinatorial methods and look to emerging technologies.

    • Gita Naseri
    •  & Mattheos A. G. Koffas
  • Article
    | Open Access

    TRANSPARENT TESTA19 (TT19) encodes a glutathione S-transferase which functions in anthocyanin stabilization and vacuolar transport. Here, by tt19 suppressor screening, the authors show that RDR6/SGS3/DCL4 siRNA pathway constituents synergistically interact with components of the flavonoid pathway to control carbon metabolism.

    • Nan Jiang
    • , Aimer Gutierrez-Diaz
    • , Eric Mukundi
    • , Yun Sun Lee
    • , Blake C. Meyers
    • , Marisa S. Otegui
    •  & Erich Grotewold
  • Article
    | Open Access

    The spatial organisation of microbial communities is caused by the interplay of biotic and abiotic factors. Here the authors design a microfluidic platform to quantify the spatiotemporal parameters influencing diffusion-mediated interactions, and use this device to investigate information transmission and metabolic cross-feeding in synthetic microbial consortia.

    • Sonali Gupta
    • , Tyler D. Ross
    • , Marcella M. Gomez
    • , Job L. Grant
    • , Philip A. Romero
    •  & Ophelia S. Venturelli
  • Article
    | Open Access

    The type III secretion system (T3SS) of bacteria can be used to inject cargo into eukaryotic cells but its lack of target specificity is a disadvantage. Here the authors place the T3SS under the regulation of light by engineering optogenetic switches into the dynamic cytosolic T3SS component SctQ.

    • Florian Lindner
    • , Bailey Milne-Davies
    • , Katja Langenfeld
    • , Thorsten Stiewe
    •  & Andreas Diepold
  • Article
    | Open Access

    Noninvasive detection of molecular targets in living subjects could provide valuable insights into healthy function and disease. Here, the authors develop vasoactive imaging probes which allow wide-field in vivo mapping of nanomolar-level molecular species in rat brain.

    • Robert Ohlendorf
    • , Agata Wiśniowska
    • , Mitul Desai
    • , Ali Barandov
    • , Adrian L. Slusarczyk
    • , Nan Li
    •  & Alan Jasanoff
  • Article
    | Open Access

    Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.

    • Gloria M. Sheynkman
    • , Katharine S. Tuttle
    • , Florent Laval
    • , Elizabeth Tseng
    • , Jason G. Underwood
    • , Liang Yu
    • , Da Dong
    • , Melissa L. Smith
    • , Robert Sebra
    • , Luc Willems
    • , Tong Hao
    • , Michael A. Calderwood
    • , David E. Hill
    •  & Marc Vidal
  • Article
    | Open Access

    Detailed understanding of how cancer cells transition from a drug sensitive to a tolerant state is lacking. Here, using single cell proteomic and metabolic data the authors uncover that isogenic BRAF mutant melanoma cells can take two distinct paths to become tolerant to BRAF inhibition.

    • Yapeng Su
    • , Melissa E. Ko
    • , Hanjun Cheng
    • , Ronghui Zhu
    • , Min Xue
    • , Jessica Wang
    • , Jihoon W. Lee
    • , Luke Frankiw
    • , Alexander Xu
    • , Stephanie Wong
    • , Lidia Robert
    • , Kaitlyn Takata
    • , Dan Yuan
    • , Yue Lu
    • , Sui Huang
    • , Antoni Ribas
    • , Raphael Levine
    • , Garry P. Nolan
    • , Wei Wei
    • , Sylvia K. Plevritis
    • , Guideng Li
    • , David Baltimore
    •  & James R. Heath
  • Article
    | Open Access

    Exosomes are used as disease biomarkers, but their characterization in biological samples is challenging. Here the authors achieve simultaneous characterization of size and zeta potential of individual nanoparticles and particle mixtures at physiological salinity conditions, exploiting a salt gradient in a capillary channel.

    • Martin K. Rasmussen
    • , Jonas N. Pedersen
    •  & Rodolphe Marie
  • Article
    | Open Access

    Local anesthetic sustained release systems suffer from untriggered rapid drug release upon application. Here the authors overcome this issue by covalently linking tetracaine to a polymer gel via a photo-cleavable linkage, enabling light-triggered and repeatable drug release.

    • Wei Zhang
    • , Tianjiao Ji
    • , Yang Li
    • , Yueqin Zheng
    • , Manisha Mehta
    • , Chao Zhao
    • , Andong Liu
    •  & Daniel S. Kohane
  • Article
    | Open Access

    Manipulation of genes controlling microbial shapes can affect bio-production. Here, the authors employ an optogenetic method to realize dynamic morphological engineering of E. coli replication and division and show the increased production of acetoin and poly(lactate-co-3-hydroxybutyrate).

    • Qiang Ding
    • , Danlei Ma
    • , Gao-Qiang Liu
    • , Yang Li
    • , Liang Guo
    • , Cong Gao
    • , Guipeng Hu
    • , Chao Ye
    • , Jia Liu
    • , Liming Liu
    •  & Xiulai Chen
  • Article
    | Open Access

    Lactazole A is a thiopeptide from Streptomyces lactacystinaeus, encoded by a compact 9.8 kb biosynthetic gene cluster. Here, the authors show a platform for in vitro biosynthesis of lactazole A via a combination of a flexible in vitro translation system with recombinantly produced lactazole biosynthetic enzymes.

    • Alexander A. Vinogradov
    • , Morito Shimomura
    • , Yuki Goto
    • , Taro Ozaki
    • , Shumpei Asamizu
    • , Yoshinori Sugai
    • , Hiroaki Suga
    •  & Hiroyasu Onaka
  • Article
    | Open Access

    Biofilm formation is a major problem in indwelling medical devices. Here, the authors report on the development of a magnetically responsive micro pillar surface for the controlled prevention and removal of biofilms which also increased sensitivity to antibiotics.

    • Huan Gu
    • , Sang Won Lee
    • , Joseph Carnicelli
    • , Teng Zhang
    •  & Dacheng Ren
  • Article
    | Open Access

    Arterial degeneration, closely associated with cardiovascular diseases, is driven by aging-related vascular cell-specific transcriptomics changes. This study provides a single-cell transcriptomic atlas for senile aortic and coronary arteries and underscores FOXO3A-based the transcriptional network in vasoprotection during aging.

    • Weiqi Zhang
    • , Shu Zhang
    • , Pengze Yan
    • , Jie Ren
    • , Moshi Song
    • , Jingyi Li
    • , Jinghui Lei
    • , Huize Pan
    • , Si Wang
    • , Xibo Ma
    • , Shuai Ma
    • , Hongyu Li
    • , Fei Sun
    • , Haifeng Wan
    • , Wei Li
    • , Piu Chan
    • , Qi Zhou
    • , Guang-Hui Liu
    • , Fuchou Tang
    •  & Jing Qu
  • Article
    | Open Access

    Developing noninvasive diagnostic biomarkers for lupus nephritis (LN) diagnosis is an important clinical goal. Here the authors identify urinary proteins correlated with active LN and disease severity, which differ across ethnicities but collectively outperform the current clinical method.

    • Samantha Stanley
    • , Kamala Vanarsa
    • , Samar Soliman
    • , Deena Habazi
    • , Claudia Pedroza
    • , Gabriel Gidley
    • , Ting Zhang
    • , Shree Mohan
    • , Evan Der
    • , Hemant Suryawanshi
    • , Thomas Tuschl
    • , Jill Buyon
    • , Chaim Putterman
    • , Chi Chiu Mok
    • , Michelle Petri
    • , Ramesh Saxena
    •  & Chandra Mohan
  • Article
    | Open Access

    Previous versions of photoactivatable Cre recombinase (PA-Cre) suffered from unintentional recombination in dark conditions. Here, the authors develop an improved version of PA-Cre, called PA-Cre 3.0, which shows reduced leakiness and improved efficiency upon activation, and make mouse lines that express PA-Cre 3.0 conditionally.

    • Kumi Morikawa
    • , Kazuhiro Furuhashi
    • , Carmen de Sena-Tomas
    • , Alvaro L. Garcia-Garcia
    • , Ramsey Bekdash
    • , Alison D. Klein
    • , Nicholas Gallerani
    • , Hannah E. Yamamoto
    • , Seon-Hye E. Park
    • , Grant S. Collins
    • , Fuun Kawano
    • , Moritoshi Sato
    • , Chyuan-Sheng Lin
    • , Kimara L. Targoff
    • , Edmund Au
    • , Michael C. Salling
    •  & Masayuki Yazawa
  • Article
    | Open Access

    Fermentation parameters of industrial processes are often not the ideal growth conditions for industrial microbes. Here, the authors reveal that young genes are more responsive to environmental stress than ancient genes using a new gene age assignment method and provide targeted genes for metabolic engineering.

    • Tyler W. Doughty
    • , Iván Domenzain
    • , Aaron Millan-Oropeza
    • , Noemi Montini
    • , Philip A. de Groot
    • , Rui Pereira
    • , Jens Nielsen
    • , Céline Henry
    • , Jean-Marc G. Daran
    • , Verena Siewers
    •  & John P. Morrissey
  • Article
    | Open Access

    Precise patterning of lipid-stabilised aqueous droplets is a key challenge in building synthetic tissue designs. Here, the authors show how the interactions between pairs of droplets direct the packing of droplets within 3D-printed networks, enabling the formation of synthetic tissues with high-resolution features.

    • Alessandro Alcinesio
    • , Oliver J. Meacock
    • , Rebecca G. Allan
    • , Carina Monico
    • , Vanessa Restrepo Schild
    • , Idil Cazimoglu
    • , Matthew T. Cornall
    • , Ravinash Krishna Kumar
    •  & Hagan Bayley
  • Article
    | Open Access

    A small set of promoters is used for most genetic construct design in S. cerevisiae. Here, the authors develop a predictive model of promoter activity trained on a data set of over one million sequences and use it to design large sets of high-activity promoters.

    • Benjamin J. Kotopka
    •  & Christina D. Smolke
  • Article
    | Open Access

    Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal and gene detection.

    • Mingxia Zhang
    • , Yuan Zou
    • , Xing Xu
    • , Xuebing Zhang
    • , Mingxuan Gao
    • , Jia Song
    • , Peifeng Huang
    • , Qin Chen
    • , Zhi Zhu
    • , Wei Lin
    • , Richard N. Zare
    •  & Chaoyong Yang
  • Article
    | Open Access

    Sorting insects based on sex is error prone and frequently labour intensive. Here, the authors present a drug-inducible sex separation system based on sex-specific rescue from antibotic toxicity.

    • Nikolay P. Kandul
    • , Junru Liu
    • , Alexander D. Hsu
    • , Bruce A. Hay
    •  & Omar S. Akbari
  • Article
    | Open Access

    Many industrial organisms are the result of recent or ancient allopolypoidy events. Here the authors iteratively combine the genomes of six yeast species to generate a viable hybrid.

    • David Peris
    • , William G. Alexander
    • , Kaitlin J. Fisher
    • , Ryan V. Moriarty
    • , Mira G. Basuino
    • , Emily J. Ubbelohde
    • , Russell L. Wrobel
    •  & Chris Todd Hittinger
  • Article
    | Open Access

    Artifact-free opto-electrophysiology is key for precise modulation and monitoring of individual neurons at high spatio-temporal resolution. The authors present a method for eliminating stimulation artifacts in high-density micro-LED optoelectrodes for accurate functional mapping of local circuits.

    • Kanghwan Kim
    • , Mihály Vöröslakos
    • , John P. Seymour
    • , Kensall D. Wise
    • , György Buzsáki
    •  & Euisik Yoon
  • Article
    | Open Access

    Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.

    • Yi Yu
    • , Thomas C. Leete
    • , David A. Born
    • , Lauren Young
    • , Luis A. Barrera
    • , Seung-Joo Lee
    • , Holly A. Rees
    • , Giuseppe Ciaramella
    •  & Nicole M. Gaudelli
  • Article
    | Open Access

    Targeted delivery of nanomaterials to subcellular compartments could allow precision engineering of plant function. Here, Santana et al. show that quantum dots functionalized with a rationally-designed targeting peptide are preferentially delivered to chloroplasts where they can be used to tune organellar redox status.

    • Israel Santana
    • , Honghong Wu
    • , Peiguang Hu
    •  & Juan Pablo Giraldo
  • Article
    | Open Access

    Cas9 base editors are promising tools for correcting pathogenic single nucleotide mutations. Here the authors chemically modify mRNA encoding the editor and the gRNA to enhance editing and broaden its application.

    • Tingting Jiang
    • , Jordana M. Henderson
    • , Kevin Coote
    • , Yi Cheng
    • , Hillary C. Valley
    • , Xiao-Ou Zhang
    • , Qin Wang
    • , Luke H. Rhym
    • , Yueying Cao
    • , Gregory A. Newby
    • , Hermann Bihler
    • , Martin Mense
    • , Zhiping Weng
    • , Daniel G. Anderson
    • , Anton P. McCaffrey
    • , David R. Liu
    •  & Wen Xue
  • Article
    | Open Access

    Malate dehydrogenase (MDH) is one of the key enzymes for succinic acid (SA) bioproduction. Here, the authors report biochemical and structural analyses of various MDHs to reveal amino acids influencing the specific activity and susceptibility to substrate inhibition, and achieve industrial-level SA production.

    • Jung Ho Ahn
    • , Hogyun Seo
    • , Woojin Park
    • , Jihye Seok
    • , Jong An Lee
    • , Won Jun Kim
    • , Gi Bae Kim
    • , Kyung-Jin Kim
    •  & Sang Yup Lee
  • Article
    | Open Access

    Corn fiber is a difficult feedstock to utilize due to its recalcitrant hemicellulose. Here, the authors characterize the recalcitrant structures, isolate a new bacterium to consume the hemicellulose, identify its enzymes, and show the benefit with increased conversion of corn fiber to ethanol.

    • Dhananjay Beri
    • , William S. York
    • , Lee R. Lynd
    • , Maria J. Peña
    •  & Christopher D. Herring
  • Article
    | Open Access

    Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.

    • Stefan Kol
    • , Daniel Ley
    • , Tune Wulff
    • , Marianne Decker
    • , Johnny Arnsdorf
    • , Sanne Schoffelen
    • , Anders Holmgaard Hansen
    • , Tanja Lyholm Jensen
    • , Jahir M. Gutierrez
    • , Austin W. T. Chiang
    • , Helen O. Masson
    • , Bernhard O. Palsson
    • , Bjørn G. Voldborg
    • , Lasse Ebdrup Pedersen
    • , Helene Faustrup Kildegaard
    • , Gyun Min Lee
    •  & Nathan E. Lewis
  • Article
    | Open Access

    Ribosome engineering is an emerging powerful approach for synthetic protein synthesis. Here the authors invert the Ribo-T system, using the engineered ribosome to translate the proteome while the native ribosome translates specific mRNA.

    • Nikolay A. Aleksashin
    • , Teresa Szal
    • , Anne E. d’Aquino
    • , Michael C. Jewett
    • , Nora Vázquez-Laslop
    •  & Alexander S. Mankin
  • Article
    | Open Access

    Base editors allow for the precise modification of genes. Here the authors use Target-AID to systematically test 17,000 sites across the yeast genome.

    • Philippe C. Després
    • , Alexandre K. Dubé
    • , Motoaki Seki
    • , Nozomu Yachie
    •  & Christian R. Landry
  • Article
    | Open Access

    Due to tumour microenvironment, Fenton reactions have low therapeutic efficiency. Here the authors report on the application of NIR-II hybrid semiconducting nanozymes for combined photothermal therapy and enhanced ferrotherapy with photoacoustic imaging and show application in vivo in tumour models.

    • Yuyan Jiang
    • , Xuhui Zhao
    • , Jiaguo Huang
    • , Jingchao Li
    • , Paul Kumar Upputuri
    • , He Sun
    • , Xiao Han
    • , Manojit Pramanik
    • , Yansong Miao
    • , Hongwei Duan
    • , Kanyi Pu
    •  & Ruiping Zhang
  • Article
    | Open Access

    Therapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small molecule membrane-destabilising drugs.

    • Hampus Du Rietz
    • , Hampus Hedlund
    • , Sten Wilhelmson
    • , Pontus Nordenfelt
    •  & Anders Wittrup
  • Article
    | Open Access

    Combining genome mining and heterologous expression in a genetically tractable host can lead to bioactive natural products discovery and production. Here, the authors employ this strategy for new decalin-containing diterpenoid pyrenes production by expressing native, extended, and shunt pathways in Aspergillus oryzae.

    • Kento Tsukada
    • , Shono Shinki
    • , Akiho Kaneko
    • , Kazuma Murakami
    • , Kazuhiro Irie
    • , Masatoshi Murai
    • , Hideto Miyoshi
    • , Shingo Dan
    • , Kumi Kawaji
    • , Hironori Hayashi
    • , Eiichi N. Kodama
    • , Aki Hori
    • , Emil Salim
    • , Takayuki Kuraishi
    • , Naoya Hirata
    • , Yasunari Kanda
    •  & Teigo Asai
  • Article
    | Open Access

    Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation-resistant scFv fragments.

    • Jessica S. Ebo
    • , Janet C. Saunders
    • , Paul W. A. Devine
    • , Alice M. Gordon
    • , Amy S. Warwick
    • , Bob Schiffrin
    • , Stacey E. Chin
    • , Elizabeth England
    • , James D. Button
    • , Christopher Lloyd
    • , Nicholas J. Bond
    • , Alison E. Ashcroft
    • , Sheena E. Radford
    • , David C. Lowe
    •  & David J. Brockwell
  • Perspective
    | Open Access

    The role microbes play in human health and the ability of synthetic biology to engineer microbial properties opens up new ways of treating disease. In this perspective, the authors describe the design and development of these living therapeutics.

    • Mark R. Charbonneau
    • , Vincent M. Isabella
    • , Ning Li
    •  & Caroline B. Kurtz
  • Article
    | Open Access

    Current synthetic DNA-based data storage systems have high recording costs, read-write latency and error-rates that make them uncompetitive compared to traditional digital storage. The authors use nicks in native DNA to encode data in parallel and create access sites for in-memory computations.

    • S. Kasra Tabatabaei
    • , Boya Wang
    • , Nagendra Bala Murali Athreya
    • , Behnam Enghiad
    • , Alvaro Gonzalo Hernandez
    • , Christopher J. Fields
    • , Jean-Pierre Leburton
    • , David Soloveichik
    • , Huimin Zhao
    •  & Olgica Milenkovic
  • Article
    | Open Access

    Developing cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tolerance and production.

    • Lun Yao
    • , Kiyan Shabestary
    • , Sara M. Björk
    • , Johannes Asplund-Samuelsson
    • , Haakan N. Joensson
    • , Michael Jahn
    •  & Elton P. Hudson
  • Article
    | Open Access

    To model hepatocyte function accurately in vitro, it is necessary to generate and maintain a polarized epithelium. Here, the authors describe a protocol to generate polarized human pluripotent stem cell-derived hepatocyte-like cells (HLCs) to model enteric virus production and drug secretion in vitro.

    • Viet Loan Dao Thi
    • , Xianfang Wu
    • , Rachel L. Belote
    • , Ursula Andreo
    • , Constantin N. Takacs
    • , Joseph P. Fernandez
    • , Luis Andre Vale-Silva
    • , Sarah Prallet
    • , Charlotte C. Decker
    • , Rebecca M. Fu
    • , Bingqian Qu
    • , Kunihiro Uryu
    • , Henrik Molina
    • , Mohsan Saeed
    • , Eike Steinmann
    • , Stephan Urban
    • , Roshni R. Singaraja
    • , William M. Schneider
    • , Sanford M. Simon
    •  & Charles M. Rice
  • Article
    | Open Access

    Synthetic nanopillars resembling the wing surface of certain insects display antibacterial activities. Here, Jenkins et al. show that these activities do not necessarily require bacterial lysis, and may be mediated by oxidative stress induced by deformation of the bacterial cell envelope.

    • J. Jenkins
    • , J. Mantell
    • , C. Neal
    • , A. Gholinia
    • , P. Verkade
    • , A. H. Nobbs
    •  & B. Su
  • Article
    | Open Access

    Acellular tissue engineered vessels functionalised with VEGF are coated with a layer of endothelial cells after in vivo implantation, but the source of the cells are unknown. Here the authors provide evidence that monocytes expressing VEGF receptors can transdifferentiate into endothelial cells via a macrophage intermediate.

    • Randall J. Smith Jr.
    • , Bita Nasiri
    • , Julien Kann
    • , Donald Yergeau
    • , Jonathan E. Bard
    • , Daniel D. Swartz
    •  & Stelios T. Andreadis