Biophysics

  • Article
    | Open Access

    In Parkinson’s disease (PD) the monomeric protein alpha-synuclein (aSyn) misfolds and aggregates into insoluble fibrils. Here the authors use NMR measurements and hydrogen–deuterium exchange mass spectrometry and find that the more solvent exposed the N-terminus of aSyn is, the more aggregation prone its conformation becomes, and further show how PD mutations and post translational modifications influence the extent of the N-terminus solvent exposure.

    • Amberley D. Stephens
    • , Maria Zacharopoulou
    • , Rani Moons
    • , Giuliana Fusco
    • , Neeleema Seetaloo
    • , Anass Chiki
    • , Philippa J. Woodhams
    • , Ioanna Mela
    • , Hilal A. Lashuel
    • , Jonathan J. Phillips
    • , Alfonso De Simone
    • , Frank Sobott
    •  & Gabriele S. Kaminski Schierle
  • Article
    | Open Access

    Small molecule polyamines participate in diverse aspects of cell growth and differentiation and are known to regulate ion channel gating. Here authors reveal that cellular polyamines control nicotinic acetylcholine receptor (nAChR) biogenesis, and either catabolic degradation or inhibition of polyamine production augments nAChR assembly.

    • Madhurima Dhara
    • , Jose A. Matta
    • , Min Lei
    • , Daniel Knowland
    • , Hong Yu
    • , Shenyan Gu
    •  & David S. Bredt
  • Article
    | Open Access

    DmpR is a bacterial enhancer binding protein from the AAA+ family of ATPases that binds aromatic compounds and controls the transcription of genes involved in the degradation of toxic pollutants. Here, the authors present the crystal structure of phenol-bound DmpR, which forms a tetramer and discuss its signal transduction mechanism.

    • Kwang-Hyun Park
    • , Sungchul Kim
    • , Su-Jin Lee
    • , Jee-Eun Cho
    • , Vinod Vikas Patil
    • , Arti Baban Dumbrepatil
    • , Hyung-Nam Song
    • , Woo-Chan Ahn
    • , Chirlmin Joo
    • , Seung-Goo Lee
    • , Victoria Shingler
    •  & Eui-Jeon Woo
  • Article
    | Open Access

    ESCRT-III complexes assemble in vivo inside membrane structures with a negative Gaussian curvature, but how membrane shape influences ESCRT-III polymerization remains unclear. Here authors use structural and biophysical methods to show how human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation.

    • Aurélie Bertin
    • , Nicola de Franceschi
    • , Eugenio de la Mora
    • , Sourav Maiti
    • , Maryam Alqabandi
    • , Nolwen Miguet
    • , Aurélie di Cicco
    • , Wouter H. Roos
    • , Stéphanie Mangenot
    • , Winfried Weissenhorn
    •  & Patricia Bassereau
  • Article
    | Open Access

    F1Fo ATP synthase consists of two coupled rotary molecular motors: the soluble ATPase F1 and the transmembrane Fo. Here, the authors present cryo-EM structures of E. coli ATP synthase in four discrete rotational sub-states at 3.1-3.4 Å resolution and observe a rotary sub-step of the Fo motor cring that reveals the mechanism of elastic coupling between the two rotary motors, which is essential for effective ATP synthesis.

    • Meghna Sobti
    • , James L. Walshe
    • , Di Wu
    • , Robert Ishmukhametov
    • , Yi C. Zeng
    • , Carol V. Robinson
    • , Richard M. Berry
    •  & Alastair G. Stewart
  • Article
    | Open Access

    The E46K α-synuclein mutation causes familial Parkinson’s disease. Here, the authors present the cryo-EM structure of N-terminally acetylated E46K α-synuclein fibrils and find that it is distinct from other known α-synuclein fibril structures.

    • Kun Zhao
    • , Yaowang Li
    • , Zhenying Liu
    • , Houfang Long
    • , Chunyu Zhao
    • , Feng Luo
    • , Yunpeng Sun
    • , Youqi Tao
    • , Xiao-dong Su
    • , Dan Li
    • , Xueming Li
    •  & Cong Liu
  • Article
    | Open Access

    Some kinesins exhibit off-axis power strokes but their impact on motility and force generation in microtubule overlaps has not been investigated so far. Here authors use a 3D in vitro motility assay and find that Ndc’s off-axis motor forces generate torque in antiparallel microtubules which causes microtubule twisting and coiling.

    • Aniruddha Mitra
    • , Laura Meißner
    • , Rojapriyadharshini Gandhimathi
    • , Roman Renger
    • , Felix Ruhnow
    •  & Stefan Diez
  • Article
    | Open Access

    There is a need to further improve the automation of cryo-EM sample preparation to make it more easily accessible for non-specialists, reduce sample waste and increase reproducibility. Here, the authors present VitroJet, a single device, where sub-nl volumes of samples are deposited by pin printing thus eliminating the need for sample blotting, which is followed by jet vitrification, and they show that high-resolution structures can be obtained using four standard proteins.

    • Raimond B. G. Ravelli
    • , Frank J. T. Nijpels
    • , Rene J. M. Henderikx
    • , Giulia Weissenberger
    • , Sanne Thewessem
    • , Abril Gijsbers
    • , Bart W. A. M. M. Beulen
    • , Carmen López-Iglesias
    •  & Peter J. Peters
  • Article
    | Open Access

    The α-cardiac actin M305L hypertrophic cardiomyopathy-causing mutation is located near residues that help confine tropomyosin to an inhibitory position along thin filaments. Here the authors assessed M305L actin in vivo, in vitro, and in silico to characterize emergent pathological properties and define the mechanistic basis of disease.

    • Meera C. Viswanathan
    • , William Schmidt
    • , Peter Franz
    • , Michael J. Rynkiewicz
    • , Christopher S. Newhard
    • , Aditi Madan
    • , William Lehman
    • , Douglas M. Swank
    • , Matthias Preller
    •  & Anthony Cammarato
  • Article
    | Open Access

    Proton-linked monocarboxylate transporters (MCTs) facilitate monocarboxylate efflux in glycolytically active cells and regulate transport down in glycolytically inactive cells. Here authors show a steep dependence of human MCT2 activity on substrate concentration and show the structural basis of cooperative transport.

    • Bo Zhang
    • , Qiuheng Jin
    • , Lizhen Xu
    • , Ningning Li
    • , Ying Meng
    • , Shenghai Chang
    • , Xiang Zheng
    • , Jiangqin Wang
    • , Yuan Chen
    • , Dante Neculai
    • , Ning Gao
    • , Xiaokang Zhang
    • , Fan Yang
    • , Jiangtao Guo
    •  & Sheng Ye
  • Article
    | Open Access

    Cytosolic ion gradients in growing pollen tubes are thought to be required for polar growth. Here the authors show that the Arabidopsis plasma membrane H+ ATPases, AHA6, AHA8, and AHA9, maintain tip-to-shank proton gradients, oscillations in cytosolic pH and actin organization to enable pollen tube elongation.

    • Robert D. Hoffmann
    • , Maria Teresa Portes
    • , Lene Irene Olsen
    • , Daniel Santa Cruz Damineli
    • , Maki Hayashi
    • , Custódio O. Nunes
    • , Jesper T. Pedersen
    • , Pedro T. Lima
    • , Cláudia Campos
    • , José A. Feijó
    •  & Michael Palmgren
  • Article
    | Open Access

    Neutrophils mobilize rapidly and travel through the vasculature and microcirculation en masse in response to inflammatory stimuli. Here the authors use a microfluidic device and intravital microscopy to show neutrophils move through tissues in a highly ordered pattern, taking turns to file into alternate branches at bifurcations.

    • Xiao Wang
    • , Mokarram Hossain
    • , Ania Bogoslowski
    • , Paul Kubes
    •  & Daniel Irimia
  • Article
    | Open Access

    Computer simulations of large-scale changes in membrane shape are challenging since they occur across a wide range of spatiotemporal scales. Here, authors present a multiscale algorithm that backmaps a continuum membrane model represented as a dynamically triangulated surface to its corresponding molecular model based on the coarse-grained Martini force field.

    • Weria Pezeshkian
    • , Melanie König
    • , Tsjerk A. Wassenaar
    •  & Siewert J. Marrink
  • Article
    | Open Access

    Auxin-mediated recruitment of AUX/IAAs by the F-box protein TIR1 prompts rapid AUX/IAA ubiquitylation and degradation. By resolving auxin receptor topology, the authors show that intrinsically disordered regions near the degrons of two Aux/IAA proteins reinforce complex assembly and position Aux/IAAs for ubiquitylation.

    • Michael Niemeyer
    • , Elena Moreno Castillo
    • , Christian H. Ihling
    • , Claudio Iacobucci
    • , Verona Wilde
    • , Antje Hellmuth
    • , Wolfgang Hoehenwarter
    • , Sophia L. Samodelov
    • , Matias D. Zurbriggen
    • , Panagiotis L. Kastritis
    • , Andrea Sinz
    •  & Luz Irina A. Calderón Villalobos
  • Article
    | Open Access

    The evolutionarily conserved complex, the Par proteins, regulates cell polarity. Here, the authors show that in Drosophila neuroblasts, the Par complex exhibits liquid–liquid phase separation dependent on the cell cycle.

    • Ziheng Liu
    • , Ying Yang
    • , Aihong Gu
    • , Jiawen Xu
    • , Ying Mao
    • , Haojie Lu
    • , Weiguo Hu
    • , Qun-Ying Lei
    • , Zhouhua Li
    • , Mingjie Zhang
    • , Yu Cai
    •  & Wenyu Wen
  • Article
    | Open Access

    The existence, nature and biological relevance of mechanoradicals in proteins are unknown. Here authors show that mechanical stress on collagen produces radicals and subsequently reactive oxygen species and suggest that collagen I evolved as a radical sponge against mechano-oxidative damage.

    • Christopher Zapp
    • , Agnieszka Obarska-Kosinska
    • , Benedikt Rennekamp
    • , Markus Kurth
    • , David M. Hudson
    • , Davide Mercadante
    • , Uladzimir Barayeu
    • , Tobias P. Dick
    • , Vasyl Denysenkov
    • , Thomas Prisner
    • , Marina Bennati
    • , Csaba Daday
    • , Reinhard Kappl
    •  & Frauke Gräter
  • Article
    | Open Access

    The contribution of central and peripheral channels of nuclear pores to transport of transmembrane proteins is unclear. Here the authors show that most inner nuclear membrane proteins use only peripheral channels, but some extend nuclear localization signals into the central channel for directed nuclear transport.

    • Krishna C. Mudumbi
    • , Rafal Czapiewski
    • , Andrew Ruba
    • , Samuel L. Junod
    • , Yichen Li
    • , Wangxi Luo
    • , Christina Ngo
    • , Valentina Ospina
    • , Eric C. Schirmer
    •  & Weidong Yang
  • Article
    | Open Access

    Potassium (K+) channels, such as MthK, are essentional for many biological processes, but how lipid-protein interactions regulate ion permeation of K+ channels remained unclear. Here authors conducted molecular dynamics simulations of MthK and observed different ion permeation rates of MthK in membranes with different properties.

    • Ruo-Xu Gu
    •  & Bert L. de Groot
  • Article
    | Open Access

    Dpr (Defective proboscis extension response) and DIP (Dpr Interacting Proteins) are immunoglobulin-like cell-cell adhesion proteins that form highly specific pairwise interactions, which control synaptic connectivity during Drosophila development. Here, the authors combine a computational approach with binding affinity measurements and find that DIP/Dpr binding specificity is controlled by negative constraints that interfere with non-cognate binding.

    • Alina P. Sergeeva
    • , Phinikoula S. Katsamba
    • , Filip Cosmanescu
    • , Joshua J. Brewer
    • , Goran Ahlsen
    • , Seetha Mannepalli
    • , Lawrence Shapiro
    •  & Barry Honig
  • Article
    | Open Access

    The Na+-pumping KR2 rhodopsin from Krokinobacter eikastus is a light-driven non-proton cation pump whose mechanism of pumping remains to be understood. Here authors solved crystal structures of the O-intermediate state of the pentameric form of KR2 and its D116N and H30A mutants, which sheds light on the mechanism of non-proton cation light-driven pumping.

    • Kirill Kovalev
    • , Roman Astashkin
    • , Ivan Gushchin
    • , Philipp Orekhov
    • , Dmytro Volkov
    • , Egor Zinovev
    • , Egor Marin
    • , Maksim Rulev
    • , Alexey Alekseev
    • , Antoine Royant
    • , Philippe Carpentier
    • , Svetlana Vaganova
    • , Dmitrii Zabelskii
    • , Christian Baeken
    • , Ilya Sergeev
    • , Taras Balandin
    • , Gleb Bourenkov
    • , Xavier Carpena
    • , Roeland Boer
    • , Nina Maliar
    • , Valentin Borshchevskiy
    • , Georg Büldt
    • , Ernst Bamberg
    •  & Valentin Gordeliy
  • Article
    | Open Access

    The mechanism of PARP1-dependent poly-ADP-ribosylation in response to DNA damage is still under debate. Here, the authors use ATR-FTIR spectroscopy to provide time-resolved insights into the molecular details of this process under near physiological conditions.

    • Annika Krüger
    • , Alexander Bürkle
    • , Karin Hauser
    •  & Aswin Mangerich
  • Article
    | Open Access

    Precise patterning of lipid-stabilised aqueous droplets is a key challenge in building synthetic tissue designs. Here, the authors show how the interactions between pairs of droplets direct the packing of droplets within 3D-printed networks, enabling the formation of synthetic tissues with high-resolution features.

    • Alessandro Alcinesio
    • , Oliver J. Meacock
    • , Rebecca G. Allan
    • , Carina Monico
    • , Vanessa Restrepo Schild
    • , Idil Cazimoglu
    • , Matthew T. Cornall
    • , Ravinash Krishna Kumar
    •  & Hagan Bayley
  • Article
    | Open Access

    Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.

    • Jaime Andrés Rivas-Pardo
    • , Yong Li
    • , Zsolt Mártonfalvi
    • , Rafael Tapia-Rojo
    • , Andreas Unger
    • , Ángel Fernández-Trasancos
    • , Elías Herrero-Galán
    • , Diana Velázquez-Carreras
    • , Julio M. Fernández
    • , Wolfgang A. Linke
    •  & Jorge Alegre-Cebollada
  • Article
    | Open Access

    Super-resolution microscopy typically requires high laser powers which can induce photobleaching and degrade image quality. Here the authors augment structured illumination microscopy (SIM) with deep learning to reduce the number of raw images required and boost its performance under low light conditions.

    • Luhong Jin
    • , Bei Liu
    • , Fenqiang Zhao
    • , Stephen Hahn
    • , Bowei Dong
    • , Ruiyan Song
    • , Timothy C. Elston
    • , Yingke Xu
    •  & Klaus M. Hahn
  • Article
    | Open Access

    Aberrant chromatin structure is often found in cancer. Here, the authors optimise super-resolution microscopy for pathological tissue and discovered a significant decompaction of chromatin folding in early carcinogenesis prior to tumour formation.

    • Jianquan Xu
    • , Hongqiang Ma
    • , Hongbin Ma
    • , Wei Jiang
    • , Christopher A. Mela
    • , Meihan Duan
    • , Shimei Zhao
    • , Chenxi Gao
    • , Eun-Ryeong Hahm
    • , Santana M. Lardo
    • , Kris Troy
    • , Ming Sun
    • , Reet Pai
    • , Donna B. Stolz
    • , Lin Zhang
    • , Shivendra Singh
    • , Randall E. Brand
    • , Douglas J. Hartman
    • , Jing Hu
    • , Sarah J. Hainer
    •  & Yang Liu
  • Article
    | Open Access

    Mass photometry is a label-free optical approach capable of detecting, imaging and accurately measuring the mass of single biomolecules in solution. Here, the authors demonstrate the potential of mass photometry for quantitatively characterizing sample heterogeneity of purified protein complexes with implications for structural studies specifically and in vitro studies more generally.

    • Adar Sonn-Segev
    • , Katarina Belacic
    • , Tatyana Bodrug
    • , Gavin Young
    • , Ryan T. VanderLinden
    • , Brenda A. Schulman
    • , Johannes Schimpf
    • , Thorsten Friedrich
    • , Phat Vinh Dip
    • , Thomas U. Schwartz
    • , Benedikt Bauer
    • , Jan-Michael Peters
    • , Weston B. Struwe
    • , Justin L. P. Benesch
    • , Nicholas G. Brown
    • , David Haselbach
    •  & Philipp Kukura
  • Article
    | Open Access

    Lipoprotein Lpp provides a covalent crosslink between the outer membrane and the peptidoglycan in E. coli. Here, the authors use atomic force microscopy to show that Lpp contributes to cell envelope stiffness by covalently connecting the two layers and by controlling the width of the periplasmic space.

    • Marion Mathelié-Guinlet
    • , Abir T. Asmar
    • , Jean-François Collet
    •  & Yves F. Dufrêne
  • Article
    | Open Access

    Energy coupling factor (ECF) transporters are responsible for the uptake of micronutrients and consist of an integral membrane unit, the S-component, which confers substrate specificity. Here, authors present multi-scale molecular dynamics simulations and in vitro experiments to study the molecular toppling mechanism of the S-component of a folate-specific ECF transporter.

    • Ignacio Faustino
    • , Haleh Abdizadeh
    • , Paulo C. T. Souza
    • , Aike Jeucken
    • , Weronika K. Stanek
    • , Albert Guskov
    • , Dirk J. Slotboom
    •  & Siewert J. Marrink
  • Article
    | Open Access

    The phase separation of two species of associating polymers is suppressed by a magic-number effect for certain combinations of the numbers of binding sites. Here the authors use lattice simulations and analytical calculations to show that this magic-number effect can be greatly enhanced if one component has a rigid shape.

    • Bin Xu
    • , Guanhua He
    • , Benjamin G. Weiner
    • , Pierre Ronceray
    • , Yigal Meir
    • , Martin C. Jonikas
    •  & Ned S. Wingreen
  • Article
    | Open Access

    Acyl-acyl carrier protein reductase (AAR) and aldehyde deformylating oxygenase (ADO) are the two enzymes in a cyanobacterial alkane biosynthesis pathway that is of interest for biofuel production. Here the authors provide insights into the catalytic mechanisms of AAR and the coupling between the two enzymes by determining the crystal structures of AAR alone and three AAR–ADO complexes with various bound ligands.

    • Yu Gao
    • , Hongmei Zhang
    • , Minrui Fan
    • , Chenjun Jia
    • , Lifang Shi
    • , Xiaowei Pan
    • , Peng Cao
    • , Xuelin Zhao
    • , Wenrui Chang
    •  & Mei Li
  • Article
    | Open Access

    Near-infrared (NIR) fluorophores have attracted interest for bioimaging; yet availability, biocompatibility and application can be an issue. Here, the authors report on the development of Egyptian Blue nanosheets with high NIR fluorescence and photostability demonstrating bioimaging applications in vivo.

    • Gabriele Selvaggio
    • , Alexey Chizhik
    • , Robert Nißler
    • , llyas Kuhlemann
    • , Daniel Meyer
    • , Loan Vuong
    • , Helen Preiß
    • , Niklas Herrmann
    • , Florian A. Mann
    • , Zhiyi Lv
    • , Tabea A. Oswald
    • , Alexander Spreinat
    • , Luise Erpenbeck
    • , Jörg Großhans
    • , Volker Karius
    • , Andreas Janshoff
    • , Juan Pablo Giraldo
    •  & Sebastian Kruss
  • Article
    | Open Access

    SNX-BAR proteins are a family of PX and BAR domain-containing proteins with pivotal roles in trafficking processes. Here authors present the cryo-EM structure of the full-length fungal SNX-BAR Mvp1, which is an autoinhibited tetramer and provides critical insight into SNX-BAR function and regulation.

    • Dapeng Sun
    • , Natalia V. Varlakhanova
    • , Bryan A. Tornabene
    • , Rajesh Ramachandran
    • , Peijun Zhang
    •  & Marijn G. J. Ford
  • Article
    | Open Access

    Mfd recognizes stalled transcriptional complexes at sites of lesions and recruits the nucleotide excision repair proteins (UvrAB) to the site. Here the authors use live cell imaging in E. coli to demonstrate that coordinated ATP hydrolysis by UvrA and loading of UvrB on DNA facilitate the dissociation of Mfd from the handoff complex.

    • Han Ngoc Ho
    • , Antoine M. van Oijen
    •  & Harshad Ghodke
  • Article
    | Open Access

    How and which cell surface molecules are taken up by clathrin-independent endocytosis is an ongoing area of research. Here, the authors show that the tumor marker CD166 is a clathrin-independent cargo that is taken up by endophilin-A3 and galectin-8, which regulates cancer cell migration.

    • Henri-François Renard
    • , François Tyckaert
    • , Cristina Lo Giudice
    • , Thibault Hirsch
    • , Cesar Augusto Valades-Cruz
    • , Camille Lemaigre
    • , Massiullah Shafaq-Zadah
    • , Christian Wunder
    • , Ruddy Wattiez
    • , Ludger Johannes
    • , Pierre van der Bruggen
    • , David Alsteens
    •  & Pierre Morsomme
  • Article
    | Open Access

    Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are essential for rhythmic activity in the heart and brain. Here authors reverse the voltage dependence of HCN channels by mutating only two residues located at the interface between the voltage sensor and the pore domain.

    • Rosamary Ramentol
    • , Marta E. Perez
    •  & H. Peter Larsson
  • Article
    | Open Access

    Epithelial cell monolayers show remarkable displacement and velocity correlations over distances of ten or more cell sizes. Here the authors show that cell motility coupled to the collective elastic modes of the cell sheet is sufficient to produce characteristic swirl-like correlations.

    • Silke Henkes
    • , Kaja Kostanjevec
    • , J. Martin Collinson
    • , Rastko Sknepnek
    •  & Eric Bertin
  • Article
    | Open Access

    The actin homolog MreB directs cell-wall insertion and maintains cell shape in many rod-shaped bacteria. Here, Shi et al. perform molecular dynamics simulations for MreB to extract mechanical parameters for inputs into a coarse-grained biophysical polymer model that predicts MreB filament properties.

    • Handuo Shi
    • , David A. Quint
    • , Gregory M. Grason
    • , Ajay Gopinathan
    •  & Kerwyn Casey Huang
  • Article
    | Open Access

    It is not fully understood how XPA interacts with a DNA lesion during nucleotide excision repair. Here, the authors use single molecule analysis to study XPA–DNA interactions, including the DNA bend angle, protein stoichiometry, and diffusive properties during damage search.

    • Emily C. Beckwitt
    • , Sunbok Jang
    • , Isadora Carnaval Detweiler
    • , Jochen Kuper
    • , Florian Sauer
    • , Nina Simon
    • , Johanna Bretzler
    • , Simon C. Watkins
    • , Thomas Carell
    • , Caroline Kisker
    •  & Bennett Van Houten
  • Article
    | Open Access

    Proper membrane physiology requires maintenance of a narrow range of physicochemical properties, which must be buffered from external perturbations. Here, authors report lipidomic remodeling to preserve membrane physical properties upon exogenous polyunsaturated fatty acids exposure.

    • Kandice R. Levental
    • , Eric Malmberg
    • , Jessica L. Symons
    • , Yang-Yi Fan
    • , Robert S. Chapkin
    • , Robert Ernst
    •  & Ilya Levental
  • Article
    | Open Access

    The production of microparticles with complex geometries for biotechnological use historically requires sophisticated fabrication techniques. Here, the authors create complex particle geometries by exploiting the metastable region of the phase diagram of thermally responsive intrinsically disordered proteins within microdroplets.

    • Stefan Roberts
    • , Vincent Miao
    • , Simone Costa
    • , Joseph Simon
    • , Garrett Kelly
    • , Tejank Shah
    • , Stefan Zauscher
    •  & Ashutosh Chilkoti
  • Article
    | Open Access

    Tripartite-motif containing (TRIM) proteins modulate cellular responses to viral infection. Here the authors use molecular dynamics simulations to demonstrate that TRIM5α uses a two-dimensional lattice hopping mechanism to aggregate on the HIV capsid surface and initiate lattice growth.

    • Alvin Yu
    • , Katarzyna A. Skorupka
    • , Alexander J. Pak
    • , Barbie K. Ganser-Pornillos
    • , Owen Pornillos
    •  & Gregory A. Voth
  • Article
    | Open Access

    Nature has developed the ability to produce a wide range of optical effects most notably in the butterfly wing. Here, the authors report on the analysis of the structures responsible for ultra-black coloration across different butterflies and combine this with modelling to identify the key characteristics

    • Alexander L. Davis
    • , H. Frederik Nijhout
    •  & Sönke Johnsen
  • Article
    | Open Access

    Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications. Here the authors present an array of stably folding Mango II aptamers for imaging of coding and non-coding RNAs at single-molecule resolution, in both live and fixed cells.

    • Adam D. Cawte
    • , Peter J. Unrau
    •  & David S. Rueda
  • Article
    | Open Access

    Protein motion in crystals causes diffuse X-ray scattering, which so far has been very challenging to measure and interpret. Here the authors present a finely sampled diffuse scattering map from triclinic lysozyme, which allows them to resolve inter- and intramolecular correlations and they further analyze the maps using all-atom molecular dynamics simulations and simple vibrational models, revealing the contribution of internal protein motion.

    • Steve P. Meisburger
    • , David A. Case
    •  & Nozomi Ando
  • Article
    | Open Access

    T4 Lysozyme (T4L) is a model protein whose structure is extensively studied. Here the authors combine single-molecule and ensemble FRET measurements, FRET-positioning and screening and EPR spectroscopy to study the structural dynamics of T4L and describe its conformational landscape during the catalytic cycle by an extended Michaelis–Menten mechanism and identify an excited conformational state of the enzyme.

    • Hugo Sanabria
    • , Dmitro Rodnin
    • , Katherina Hemmen
    • , Thomas-Otavio Peulen
    • , Suren Felekyan
    • , Mark R. Fleissner
    • , Mykola Dimura
    • , Felix Koberling
    • , Ralf Kühnemuth
    • , Wayne Hubbell
    • , Holger Gohlke
    •  & Claus A. M. Seidel
  • Article
    | Open Access

    Galectin-3 consists of an unstructured N-terminal domain (NTD) and a structured carbohydrate-recognition domain and agglutinates neutrophils and glycosylated molecules in the extracellular milieu. Here the authors combine biophysical and biochemical experiments with NMR measurements and show that the galectin-3 NTD undergoes liquid-liquid phase separation (LLPS) and agglutinates other molecules through this process.

    • Yi-Ping Chiu
    • , Yung-Chen Sun
    • , De-Chen Qiu
    • , Yu-Hao Lin
    • , Yin-Quan Chen
    • , Jean-Cheng Kuo
    •  & Jie-rong Huang
  • Article
    | Open Access

    The movement of cytoplasmic dynein on microtubule tracks is coordinated by the microtubule-binding domain (MTBD) and the ATPase domain via a coiled-coil stalk. Here authors use NMR and cryo-EM and suggest that the communication between the ATPase-domain and MTBD is achieved by sliding of the stalk α-helix by a half-turn or one-turn.

    • Noritaka Nishida
    • , Yuta Komori
    • , Osamu Takarada
    • , Atsushi Watanabe
    • , Satoko Tamura
    • , Satoshi Kubo
    • , Ichio Shimada
    •  & Masahide Kikkawa
  • Article
    | Open Access

    Neurotransmitter:sodium symporters (NSS) serve as targets for drugs including antidepressants and psychostimulants. Here authors report the X-ray structure of the prokaryotic NSS member, LeuT, in a Na+/substrate-bound, inward-facing occluded conformation which is a key intermediate in the LeuT transport cycle.

    • Kamil Gotfryd
    • , Thomas Boesen
    • , Jonas S. Mortensen
    • , George Khelashvili
    • , Matthias Quick
    • , Daniel S. Terry
    • , Julie W. Missel
    • , Michael V. LeVine
    • , Pontus Gourdon
    • , Scott C. Blanchard
    • , Jonathan A. Javitch
    • , Harel Weinstein
    • , Claus J. Loland
    • , Poul Nissen
    •  & Ulrik Gether