Featured
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Review Article |
In vivo NIR-II fluorescence imaging for biology and medicine
A review of NIR-II fluorescence imaging is presented, with a focus on fluorophores, probes and imaging techniques.
- Feifei Wang
- , Yeteng Zhong
- & Hongjie Dai
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Article |
Dynamic monitoring of oscillatory enzyme activity of individual live bacteria via nanoplasmonic optical antennas
Nanoplasmonic antennas enable label-free monitoring of bacterial enzymes released via outer membrane vesicles. Real-time monitoring reveals the oscillatory behaviour of enzymatic release from individual bacteria as well as the effects of coupled oscillation from neighbouring bacteria.
- Dengyun Lu
- , Guoshuai Zhu
- & Hongbao Xin
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Article |
Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging
Super-resolution imaging based on autocorrelation with two-step deconvolution (SACD) enables recording super-resolution images with 128-nm spatial resolution over a field of view of 2.0 mm × 1.4 mm within a 10-min acquisition time.
- Weisong Zhao
- , Shiqun Zhao
- & Haoyu Li
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Article
| Open Access
Brilliant whiteness in shrimp from ultra-thin layers of birefringent nanospheres
The strong birefringence of liquid crystalline nanospheres in the body of the Pacific cleaner shrimp enables brilliant whiteness by overcoming undesirable optical crowding effects.
- Tali Lemcoff
- , Lotem Alus
- & Benjamin A. Palmer
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Article |
High-gain and high-speed wavefront shaping through scattering media
The combination of optical phase conjugation and light amplification enables wavefront shaping with simultaneously optimized operational speed, number of control degrees of freedom and energy of the focused wavefront. Shaping with a 10 μs latency time over about 106 control modes and energy gain approaching unity is demonstrated.
- Zhongtao Cheng
- , Chengmingyue Li
- & Lihong V. Wang
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Article |
Deep laser microscopy using optical clearing by ultrasound-induced gas bubbles
Optical clearing based on ultrasound-induced gas bubbles offers new opportunities for deeper laser scanning microscopy of biological tissue.
- Haemin Kim
- , Sangyeon Youn
- & Jin Ho Chang
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Obituary |
In memory of Watt Wetmore Webb
Webb’s work helped fundamentally reshape basic research and advanced manufacturing in the generation and application of photonics across disciplines, from fundamental and applied physics to the biosciences.
- Jeffrey Squier
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Article |
High-brightness self-seeded X-ray free-electron laser covering the 3.5 keV to 14.6 keV range
A hard X-ray self-seeded X-ray free-electron laser at the Pohang Accelerator Laboratory provides X-ray pulses with peak brightness of 3.2 × 1035 photons s–1 mm–2 mrad–2 0.1%BW–1 at 9.7 keV and a very small shot-to-shot electron energy jitter of 0.012%.
- Inhyuk Nam
- , Chang-Ki Min
- & Heung-Sik Kang
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Article |
Intensity-based holographic imaging via space-domain Kramers–Kronig relations
An intensity-based holographic imaging via space-domain Kramers–Kronig relations is presented, allowing the phase image of an object to be obtained directly from a single intensity measurement with oblique illumination.
- YoonSeok Baek
- & YongKeun Park
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Article |
Nanometric axial localization of single fluorescent molecules with modulated excitation
Adapting the amplitude-modulated light detection and ranging approach to super-resolution microscopy offers a typical axial localization precision of 6.8 nm over the entire field of view and the axial capture range, enabling imaging of biological samples by up to several micrometres in depth.
- Pierre Jouchet
- , Clément Cabriel
- & Sandrine Lévêque-Fort
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News & Views |
High-speed 3D mapping of nonlinear structures
Directly relating the complex second-harmonic-generation field to the second-order susceptibility tensor allows tomographic imaging of nonlinear optical contrast at high frame rates.
- Paul J. Campagnola
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News & Views |
Exploiting sound and noise
A correlation method that combines ultrasound and fluorescence enables imaging in strongly scattering environments.
- Allard P. Mosk
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Article |
Monitoring contractility in cardiac tissue with cellular resolution using biointegrated microlasers
The incorporation of microsphere lasers into heart cells allows all-optical recording of cardiac contraction with cellular resolution. [This summary has been amended from ‘microdisk’ to ‘microsphere’ lasers.]
- Marcel Schubert
- , Lewis Woolfson
- & Malte C. Gather
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Article |
Harmonic optical tomography of nonlinear structures
A tomographiac approach to second-harmonic-generation imaging on nonlinear structures is demonstrated, with experiments and three-dimensional reconstructions on a beta-barium borate crystal and various biological specimens performed.
- Chenfei Hu
- , Jeffrey J. Field
- & Gabriel Popescu
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Article |
Fluorescence imaging through dynamic scattering media with speckle-encoded ultrasound-modulated light correlation
Combining the advantages of ultrasound and light for fluorescence imaging, an imaging technique termed fluorescence and ultrasound-modulated light correlation, or FLUX, that leverages the dynamic nature of the medium is reported to uniquely resolve fluorophore distribution even when the speckles decorrelate fast.
- Haowen Ruan
- , Yan Liu
- & Changhuei Yang
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Article |
Snapshot photoacoustic topography through an ergodic relay for high-throughput imaging of optical absorption
A low-cost high-throughput photoacoustic imaging based on an ergodic relay coupled with a single-element ultrasonic transducer that can capture a wide-field image with only a single laser shot is demonstrated.
- Yang Li
- , Lei Li
- & Lihong V. Wang
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Article |
Higher-order coherent anti-Stokes Raman scattering microscopy realizes label-free super-resolution vibrational imaging
Higher-order (fifth and seventh order) coherent anti-Stokes Raman scattering microscopy is demonstrated to break the diffraction limit for label-free super-resolution vibrational imaging for live cells such as HeLa and buccal cells.
- Li Gong
- , Wei Zheng
- & Zhiwei Huang
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Article |
Optical coherence refraction tomography
By synthesizing undistorted cross-sectional image reconstructions from multiple conventional images acquired with angular diversity, optical coherence refraction tomography offers greater than threefold improvement in lateral resolution and speckle reduction in imaging tissue ultrastructure, and reconstructs the tissue’s internal refractive index distribution.
- Kevin C. Zhou
- , Ruobing Qian
- & Joseph A. Izatt
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News & Views |
Overcoming the colour barrier
High-efficiency, time-domain, near-infrared fluorophores provide multiplexed colour channels for distinct deep bioimaging.
- Shoujun Zhu
- & Xiaoyuan Chen
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Article |
Wavelength-encoded laser particles for massively multiplexed cell tagging
Intracellular laser particles based on silica-coated semiconductor microcavities with distinct emission wavelengths allow real-time tracking of thousands of cells in a tumour model.
- Nicola Martino
- , Sheldon J. J. Kwok
- & Seok-Hyun Yun
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Comment |
So much more than paper
As the most abundant biopolymer on Earth since it can be found in every plant cell wall, cellulose has emerged as an ideal candidate for the development of renewable and biodegradable photonic materials, substituting conventional pigments.
- Bruno Frka-Petesic
- & Silvia Vignolini
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Letter |
High-sensitivity imaging of time-domain near-infrared light transducer
By time-shifting short-pulse excitation photon energy into prolonged luminescent emission in the time domain, both the number of light signal transducers in sub-15 nm nanoparticles and the near-infrared-in to near-infrared-out conversion efficiency can be maximized, advancing in vivo optical bioimaging.
- Yuyang Gu
- , Zhiyong Guo
- & Fuyou Li
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Article |
Ultraviolet–visible–near-infrared optical properties of amyloid fibrils shed light on amyloidogenesis
Two optical signatures of amyloid fibres—luminescence in the blue and a near-infrared signal, which can be observed in in vitro and in vivo tissues—are reported. The findings allow for staining-free characterization of amyloid deposits in human samples and could open the door to innovative diagnostic strategies for neurodegenerative diseases.
- Jonathan Pansieri
- , Véronique Josserand
- & Vincent Forge
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News & Views |
Nanoparticles give mice infrared vision
The injection of rare-earth-doped upconversion nanoparticles into the eyes of mice allows them to visualize near-infrared light with a wavelength of ~1 μm.
- Guochen Bao
- & Dayong Jin
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News & Views |
Innovation in India
Low-cost spectroscopy, mobile phone-based imaging and laser-processed paper sensors are all examples of how photonics could help tackle India’s healthcare and environmental monitoring challenges.
- Oliver Graydon
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Comment |
Life in the light
Osamu Shimomura’s 90-year life came to an end on 19 October 2018. Throughout his long and exceedingly fruitful career, the Japanese marine biologist and chemist passionately explored the phenomenon of bioluminescence in living organisms, earning a Nobel Prize in the process.
- Yasushi Hiraoka
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News & Views |
The super-resolution debate
In the quest for nanoscopy with super-resolution, consensus from the imaging community is that super-resolution is not always needed and that scientists should choose an imaging technique based on their specific application.
- Rachel Won
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Article |
Combined multi-plane phase retrieval and super-resolution optical fluctuation imaging for 4D cell microscopy
By combining the sensitivity and high temporal resolution of phase imaging with the specificity and high spatial resolution of fluorescence microscopy, a 4D microscope is demonstrated that visualizes in three dimensions the fast cellular processes in living cells at up to 200 Hz.
- A. Descloux
- , K. S. Grußmayer
- & T. Lasser
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Article |
Three-dimensional holographic optical manipulation through a high-numerical-aperture soft-glass multimode fibre
Through a high-numerical-aperture multimode fibre, real-time manipulation of three-dimensional arrangements of micro-objects and manipulation inside inaccessible cavities are shown. The approach is useful for imaging deep inside living tissues and complex environments.
- Ivo T. Leite
- , Sergey Turtaev
- & Tomáš Čižmár
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Article |
Scattering compensation by focus scanning holographic aberration probing (F-SHARP)
A non-invasive scattering compensation method, termed F-SHARP, gives direct access to the phase and amplitude of the electric-field point spread function, enabling fast and high-resolution correction of aberrations and scattering in living tissue.
- Ioannis N. Papadopoulos
- , Jean-Sébastien Jouhanneau
- & Benjamin Judkewitz
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News & Views |
Making light work of crystal growth
A new method of stimulating crystal growth by laser light could enhance the way we make materials.
- Andrew J. Alexander
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Letter |
Super-resolution imaging of multiple cells by optimized flat-field epi-illumination
An epi-illumination system based on microlens arrays enables field-independent imaging of multiple cells with nanoscale resolution and large field of views.
- Kyle M. Douglass
- , Christian Sieben
- & Suliana Manley
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Letter |
In vivo cellular-resolution retinal imaging in infants and children using an ultracompact handheld probe
A handheld probe enables retinal imaging in children.
- Francesco LaRocca
- , Derek Nankivil
- & Joseph A. Izatt
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Editorial |
Crossroads
The merger of biology and optics was the focus of new sessions at SPIE Photonics West 2016. The conference attracted over 20,000 attendees.
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Random-access wide-field mesoscopy for centimetre-scale imaging of biodynamics with subcellular resolution
Tracking tiny proteins sans fluorescence
Shrimp eye scattering
Plasmon-enabled disinfection
Fabric optoelectronics
Global position by polarization
Consistent disorder
Graded-index squid eyes
Order in photosynthesis
Bright sponges
Cell oscillators