Biophotonics

  • Article |

    The incorporation of microsphere lasers into heart cells allows all-optical recording of cardiac contraction with cellular resolution. [This summary has been amended from ‘microdisk’ to ‘microsphere’ lasers.]

    • Marcel Schubert
    • , Lewis Woolfson
    • , Isla R. M. Barnard
    • , Amy M. Dorward
    • , Becky Casement
    • , Andrew Morton
    • , Gavin B. Robertson
    • , Paul L. Appleton
    • , Gareth B. Miles
    • , Carl S. Tucker
    • , Samantha J. Pitt
    •  & Malte C. Gather
  • Article |

    A tomographiac approach to second-harmonic-generation imaging on nonlinear structures is demonstrated, with experiments and three-dimensional reconstructions on a beta-barium borate crystal and various biological specimens performed.

    • Chenfei Hu
    • , Jeffrey J. Field
    • , Varun Kelkar
    • , Benny Chiang
    • , Keith Wernsing
    • , Kimani C. Toussaint
    • , Randy A. Bartels
    •  & Gabriel Popescu
  • Article |

    Combining the advantages of ultrasound and light for fluorescence imaging, an imaging technique termed fluorescence and ultrasound-modulated light correlation, or FLUX, that leverages the dynamic nature of the medium is reported to uniquely resolve fluorophore distribution even when the speckles decorrelate fast.

    • Haowen Ruan
    • , Yan Liu
    • , Jian Xu
    • , Yujia Huang
    •  & Changhuei Yang
  • Article |

    By synthesizing undistorted cross-sectional image reconstructions from multiple conventional images acquired with angular diversity, optical coherence refraction tomography offers greater than threefold improvement in lateral resolution and speckle reduction in imaging tissue ultrastructure, and reconstructs the tissue’s internal refractive index distribution.

    • Kevin C. Zhou
    • , Ruobing Qian
    • , Simone Degan
    • , Sina Farsiu
    •  & Joseph A. Izatt
  • Article |

    Intracellular laser particles based on silica-coated semiconductor microcavities with distinct emission wavelengths allow real-time tracking of thousands of cells in a tumour model.

    • Nicola Martino
    • , Sheldon J. J. Kwok
    • , Andreas C. Liapis
    • , Sarah Forward
    • , Hoon Jang
    • , Hwi-Min Kim
    • , Sarah J. Wu
    • , Jiamin Wu
    • , Paul H. Dannenberg
    • , Sun-Joo Jang
    • , Yong-Hee Lee
    •  & Seok-Hyun Yun
  • Letter |

    By time-shifting short-pulse excitation photon energy into prolonged luminescent emission in the time domain, both the number of light signal transducers in sub-15 nm nanoparticles and the near-infrared-in to near-infrared-out conversion efficiency can be maximized, advancing in vivo optical bioimaging.

    • Yuyang Gu
    • , Zhiyong Guo
    • , Wei Yuan
    • , Mengya Kong
    • , Yulai Liu
    • , Yongtao Liu
    • , Yilin Gao
    • , Wei Feng
    • , Fan Wang
    • , Jiajia Zhou
    • , Dayong Jin
    •  & Fuyou Li
  • Article |

    Two optical signatures of amyloid fibres—luminescence in the blue and a near-infrared signal, which can be observed in in vitro and in vivo tissues—are reported. The findings allow for staining-free characterization of amyloid deposits in human samples and could open the door to innovative diagnostic strategies for neurodegenerative diseases.

    • Jonathan Pansieri
    • , Véronique Josserand
    • , Sun-Jae Lee
    • , Anaëlle Rongier
    • , Daniel Imbert
    • , Marcelle Moulin Sallanon
    • , Enikö Kövari
    • , Thomas G. Dane
    • , Charlotte Vendrely
    • , Odette Chaix-Pluchery
    • , Mélanie Guidetti
    • , Julien Vollaire
    • , Arnold Fertin
    • , Yves Usson
    • , Patrice Rannou
    • , Jean-Luc Coll
    • , Christel Marquette
    •  & Vincent Forge
  • Article |

    By combining the sensitivity and high temporal resolution of phase imaging with the specificity and high spatial resolution of fluorescence microscopy, a 4D microscope is demonstrated that visualizes in three dimensions the fast cellular processes in living cells at up to 200 Hz.

    • A. Descloux
    • , K. S. Grußmayer
    • , E. Bostan
    • , T. Lukes
    • , A. Bouwens
    • , A. Sharipov
    • , S. Geissbuehler
    • , A.-L. Mahul-Mellier
    • , H. A. Lashuel
    • , M. Leutenegger
    •  & T. Lasser
  • Article |

    Through a high-numerical-aperture multimode fibre, real-time manipulation of three-dimensional arrangements of micro-objects and manipulation inside inaccessible cavities are shown. The approach is useful for imaging deep inside living tissues and complex environments.

    • Ivo T. Leite
    • , Sergey Turtaev
    • , Xin Jiang
    • , Martin Šiler
    • , Alfred Cuschieri
    • , Philip St. J. Russell
    •  & Tomáš Čižmár
  • Article |

    A non-invasive scattering compensation method, termed F-SHARP, gives direct access to the phase and amplitude of the electric-field point spread function, enabling fast and high-resolution correction of aberrations and scattering in living tissue.

    • Ioannis N. Papadopoulos
    • , Jean-Sébastien Jouhanneau
    • , James F. A. Poulet
    •  & Benjamin Judkewitz
  • Article |

    Researchers exploit direct stochastic optical reconstruction microscopy and dedicated detection of super-critical-angle fluorescence emission to enable direct optical nanoscopy with axially localized detection.

    • N. Bourg
    • , C. Mayet
    • , G. Dupuis
    • , T. Barroca
    • , P. Bon
    • , S. Lécart
    • , E. Fort
    •  & S. Lévêque-Fort
  • Article |

    A high-resolution, broadband imaging system based on coherent anti-Stokes Raman spectroscopy performs rapid, chemically specific imaging of biological tissue. It employs three-colour excitation and operates across the entire biological window.

    • Charles H. Camp Jr
    • , Young Jong Lee
    • , John M. Heddleston
    • , Christopher M. Hartshorn
    • , Angela R. Hight Walker
    • , Jeremy N. Rich
    • , Justin D. Lathia
    •  & Marcus T. Cicerone
  • Article |

    Polymer hydrogel patches that are capable of supporting living cells and guiding light are used to perform in-vivo optical sensing and therapy in living mice. Tasks performed include toxicity testing and glucose regulation.

    • Myunghwan Choi
    • , Jin Woo Choi
    • , Seonghoon Kim
    • , Sedat Nizamoglu
    • , Sei Kwang Hahn
    •  & Seok Hyun Yun
  • Article |

    The popular organic semiconductor P3HT, which is commonly used in polymer solar cells and photodetectors, is demonstrated to be able to act as a biocompatible optoelectronic interface for the retina of blind rats.

    • Diego Ghezzi
    • , Maria Rosa Antognazza
    • , Rita Maccarone
    • , Sebastiano Bellani
    • , Erica Lanzarini
    • , Nicola Martino
    • , Maurizio Mete
    • , Grazia Pertile
    • , Silvia Bisti
    • , Guglielmo Lanzani
    •  & Fabio Benfenati
  • Letter |

    By combining the techniques of temporal focusing and generalized phase contrast researchers are able to preserve the shape of spatial patterns of light deep inside scattering brain slices. This approach is shown to photoactivate the light-sensitive protein channelrhodopsin-2 with single-cell precision and millisecond temporal resolution.

    • Eirini Papagiakoumou
    • , Aurélien Bègue
    • , Ben Leshem
    • , Osip Schwartz
    • , Brandon M. Stell
    • , Jonathan Bradley
    • , Dan Oron
    •  & Valentina Emiliani
  • Article |

    Researchers use squeezed light to track the constituents of yeast cells with a performance that overcomes the quantum noise limit. This approach allows for the utilization of low optical power, which helps to minimize cell damage.

    • Michael A. Taylor
    • , Jiri Janousek
    • , Vincent Daria
    • , Joachim Knittel
    • , Boris Hage
    • , Hans-A. Bachor
    •  & Warwick P. Bowen
  • Review Article |

    The ability to dynamically image features deep within living organisms, permitting real-time analysis of cellular structure and function, is important for biological science. This Review article discusses multiphoton microscopy capable of such analysis, along with technologies that are pushing the limits of phenomena that can be quantitatively imaged.

    • Erich E. Hoover
    •  & Jeff A. Squier
  • Letter |

    By recording digital holograms created from different illumination directions and subsequently processing them in a complex deconvolution scheme, scientists are able to capture details of living biological samples with subwavelength resolution.

    • Yann Cotte
    • , Fatih Toy
    • , Pascal Jourdain
    • , Nicolas Pavillon
    • , Daniel Boss
    • , Pierre Magistretti
    • , Pierre Marquet
    •  & Christian Depeursinge
  • Letter |

    A parallel implementation of multifocal multiphoton modulation microscopy allows simultaneous phosphorescent lifetime and intensity imaging in vivo at speeds 100 times faster than conventional configurations. Three-dimensional imaging of a phosphorescent quenching dye is also presented.

    • Scott S. Howard
    • , Adam Straub
    • , Nicholas G. Horton
    • , Demirhan Kobat
    •  & Chris Xu
  • Article |

    Video-rate imaging of various types of biological tissue is reported using stimulated Raman scattering microscopy. The label-free scheme offers molecular specificity and frame-by-frame wavelength tunability allowing the creation of 2D and 3D images of samples showing different constituents.

    • Yasuyuki Ozeki
    • , Wataru Umemura
    • , Yoichi Otsuka
    • , Shuya Satoh
    • , Hiroyuki Hashimoto
    • , Kazuhiko Sumimura
    • , Norihiko Nishizawa
    • , Kiichi Fukui
    •  & Kazuyoshi Itoh
  • Letter |

    Researchers bring together silk and photonic crystals and report the manufacturing of robust, freestanding, three-dimensional photonic crystals with different lattice constants in the structural form of an inverse opal entirely composed of silk fibroin. These silk-based inverse opals add a new dimension at the interface of nanophotonics and biological applications.

    • Sunghwan Kim
    • , Alexander N. Mitropoulos
    • , Joshua D. Spitzberg
    • , Hu Tao
    • , David L. Kaplan
    •  & Fiorenzo G. Omenetto
  • Letter |

    The mechanism by which various species of silvery fish produce almost perfect broadband, polarization-neutral reflections is revealed. The answer lies with the use of multilayers composed of two types of birefringent guanine crystals, which each have their extraordinary and ordinary refractive indices orientated in different directions.

    • T. M. Jordan
    • , J. C. Partridge
    •  & N. W. Roberts
  • Article |

    By time-sharing optical forces, researchers show that it is possible to adapt the shape of a trapping potential to the shape of an elongated helical bacterium. This approach allows the bacterium to be held and stably oriented for several minutes, which will aid investigations into the nanomechanics of single wall-less bacteria reacting to external stimuli.

    • Matthias Koch
    •  & Alexander Rohrbach
  • Letter |

    Researchers describe an optical method for switching off or modifying the light emission from cells transfected with green fluorescent protein. The scheme uses the precise delivery of femtosecond laser light to induce the release of reactive oxygen species within the cell, which bleaches the fluorescence.

    • Hao He
    • , Shiyang Li
    • , Shaoyang Wang
    • , Minglie Hu
    • , Youjia Cao
    •  & Chingyue Wang
  • Article |

    Researchers demonstrate, in both normal and degenerate rat retinas, a photovoltaic subretinal prosthesis in which the silicon photodiodes in each pixel receive power and data through pulsed near-infrared illumination.

    • Keith Mathieson
    • , James Loudin
    • , Georges Goetz
    • , Philip Huie
    • , Lele Wang
    • , Theodore I. Kamins
    • , Ludwig Galambos
    • , Richard Smith
    • , James S. Harris
    • , Alexander Sher
    •  & Daniel Palanker
  • Article |

    Inspired by thermal expansion and refractive index changes in the nanostructures of iridescent Morpho butterfly scales, scientists demonstrate upconverted mid-wave infrared detection with a temperature sensitivity of 18–62 mK and a heat-sink-free response speed of 35–40 Hz.

    • Andrew D. Pris
    • , Yogen Utturkar
    • , Cheryl Surman
    • , William G. Morris
    • , Alexey Vert
    • , Sergiy Zalyubovskiy
    • , Tao Deng
    • , Helen T. Ghiradella
    •  & Radislav A. Potyrailo
  • Article |

    Using laser-driven spinning birefringent spheres to create a localized microfluidic flow, scientists show that they can control the direction of growth of individual nerve fibres. The approach is potentially useful for the development of nerve systems, as well as for nerve repair and regeneration.

    • Tao Wu
    • , Timo A. Nieminen
    • , Samarendra Mohanty
    • , Jill Miotke
    • , Ronald L. Meyer
    • , Halina Rubinsztein-Dunlop
    •  & Michael W. Berns
  • Letter |

    Using a new form of spectroscopic optical coherence tomography, researchers demonstrate three-dimensional molecular imaging of both endogenous and exogenous chromophores with high spectral fidelity. This scheme has significant implications for a range of biomedical applications, including ophthalmology, early cancer detection and understanding fundamental disease mechanisms such as hypoxia and angiogenesis.

    • Francisco E. Robles
    • , Christy Wilson
    • , Gerald Grant
    •  & Adam Wax
  • Letter |

    Scientists demonstrate living biological lasers by pumping cells containing green fluorescent protein in a highly reflective microcavity. The researchers also investigate the thresholds and modes of their cellular lasers.

    • Malte C. Gather
    •  & Seok Hyun Yun
  • Letter |

    The ability of pulsed near-infrared laser light to pace the heart beat of a quail embryo is demonstrated, suggesting that such optical pacing may become a useful tool for developmental cardiology.

    • M. W. Jenkins
    • , A. R. Duke
    • , S. Gu
    • , Y. Doughman
    • , H. J. Chiel
    • , H. Fujioka
    • , M. Watanabe
    • , E. D. Jansen
    •  & A. M. Rollins