Biological techniques

  • Article | | open

    The resolution limitations when using the ubiquitous algorithms that process images obtained using modern techniques are not straightforward to define. Here, the authors examine the performance of localization algorithms and use spatial statistics to provide a metric for assessing the resolution limit of such algorithms.

    • Edward A. K. Cohen
    • , Anish V. Abraham
    • , Sreevidhya Ramakrishnan
    •  & Raimund J. Ober
  • Article | | open

    Mutations that modulate the activity of ion channels are essential tools to understand the biophysical determinants that control their gating. Here authors reveal the role played by a single residue in the second transmembrane domain of vertebrate and invertebrate two-pore domain potassium channels.

    • Ismail Ben Soussia
    • , Sonia El Mouridi
    • , Dawon Kang
    • , Alice Leclercq-Blondel
    • , Lamyaa Khoubza
    • , Philippe Tardy
    • , Nora Zariohi
    • , Marie Gendrel
    • , Florian Lesage
    • , Eun-Jin Kim
    • , Delphine Bichet
    • , Olga Andrini
    •  & Thomas Boulin
  • Article | | open

    Triple negative breast cancers (TNBC) disseminate and metastasise, but the clonal relationship of metastases to primary tumours is poorly understood. Here, the authors use cellular barcoding of TNBC patient-derived xenografts and track the fate of barcoded clones in primary tumours and their metastases, including after resection or chemotherapy.

    • D. Merino
    • , T. S. Weber
    • , A. Serrano
    • , F. Vaillant
    • , K. Liu
    • , B. Pal
    • , L. Di Stefano
    • , J. Schreuder
    • , D. Lin
    • , Y. Chen
    • , M. L. Asselin-Labat
    • , T. N. Schumacher
    • , D. Cameron
    • , G. K. Smyth
    • , A. T. Papenfuss
    • , G. J. Lindeman
    • , J. E. Visvader
    •  & S. H. Naik
  • Article | | open

    Meiotic crossover (CO) landscape differs inter- and intra-species, as well as between sexes. Here, the authors show that male meiosis produces more COs than female in maize and detect CO maturation inefficiency in some genetic backgrounds, which may help to improve breeding efficiency.

    • Cheng Luo
    • , Xiang Li
    • , Qinghua Zhang
    •  & Jianbing Yan
  • Article | | open

    Molecular motion has attracted a wide range of interest for different applications. Here, the authors develop nanoparticles with internal molecular motion upon near infrared absorption and use the nanoparticles for photoacoustic imaging and demonstrate this application in vivo.

    • Zheng Zhao
    • , Chao Chen
    • , Wenting Wu
    • , Fenfen Wang
    • , Lili Du
    • , Xiaoyan Zhang
    • , Yu Xiong
    • , Xuewen He
    • , Yuanjing Cai
    • , Ryan T. K. Kwok
    • , Jacky W. Y. Lam
    • , Xike Gao
    • , Pingchuan Sun
    • , David Lee Phillips
    • , Dan Ding
    •  & Ben Zhong Tang
  • Article | | open

    Wide application of quantum dot light emitting diodes (QLED) in display technology is hindered by the poor lifetime of the blue QLEDs. Here, the degradation mechanism is shown to originate from space charge accumulation in the electron-transporting layer enabling improvements in blue QLED lifetimes.

    • Song Chen
    • , Weiran Cao
    • , Taili Liu
    • , Sai-Wing Tsang
    • , Yixing Yang
    • , Xiaolin Yan
    •  & Lei Qian
  • Article | | open

    Infection of a cell with influenza B virus (IBV) often results in cell death and the role of surviving cells in pathogenesis is unclear. Here, Dumm et al. generate a recombinant IBV that activates a host-cell reporter to permanently label infected cells, and show that surviving cells are important to preserve epithelial barrier function.

    • Rebekah E. Dumm
    • , Jessica K. Fiege
    • , Barbara M. Waring
    • , Chay T. Kuo
    • , Ryan A. Langlois
    •  & Nicholas S. Heaton
  • Article | | open

    SecB homologs can be associated with stress-responsive type II toxin–antitoxin (TA) systems and form tripartite toxin-antitoxin-chaperone systems (TAC). Here the authors provide structural insights into TACs by presenting the crystal structure of the M. tuberculosis TA-associated SecB chaperone in complex with the C-terminal ChAD (chaperone addiction) extension of the antitoxin HigA1.

    • Valérie Guillet
    • , Patricia Bordes
    • , Cécile Bon
    • , Julien Marcoux
    • , Virginie Gervais
    • , Ambre Julie Sala
    • , Suzana Dos Reis
    • , Nawel Slama
    • , Israel Mares-Mejía
    • , Anne-Marie Cirinesi
    • , Laurent Maveyraud
    • , Pierre Genevaux
    •  & Lionel Mourey
  • Article | | open

    Serine/threonine phosphatases such as PP1 associate with a large array of subunit proteins, such as ASPP (apoptosis-stimulating protein of p53) to achieve selective targeting. Here authors solved the crystal structure of the human ASPP2/PP1 complex and explain how ASPP2 can distinguish between PP1 isoforms.

    • M. Teresa Bertran
    • , Stéphane Mouilleron
    • , Yanxiang Zhou
    • , Rakhi Bajaj
    • , Federico Uliana
    • , Ganesan Senthil Kumar
    • , Audrey van Drogen
    • , Rebecca Lee
    • , Jennifer J. Banerjee
    • , Simon Hauri
    • , Nicola O’Reilly
    • , Matthias Gstaiger
    • , Rebecca Page
    • , Wolfgang Peti
    •  & Nicolas Tapon
  • Article | | open

    Negative selection removes deleterious genetic variation, and can influence genetic architectures and evolution of complex traits. Here, the authors analyze data from 25 UK Biobank diseases and complex traits, and quantify frequency-dependent genetic architectures which suggests actions of negative selection.

    • Armin P. Schoech
    • , Daniel M. Jordan
    • , Po-Ru Loh
    • , Steven Gazal
    • , Luke J. O’Connor
    • , Daniel J. Balick
    • , Pier F. Palamara
    • , Hilary K. Finucane
    • , Shamil R. Sunyaev
    •  & Alkes L. Price
  • Article | | open

    Pseudomonas aeruginosa releases redox-active metabolites called phenazines. Here, the authors use metabolic imaging by stimulated Raman scattering microscopy to show that phenazines antagonize the effects of antibiotics on P. aeruginosa biofilms by modulating bacterial metabolism.

    • Konstanze T. Schiessl
    • , Fanghao Hu
    • , Jeanyoung Jo
    • , Sakila Z. Nazia
    • , Bryan Wang
    • , Alexa Price-Whelan
    • , Wei Min
    •  & Lars E. P. Dietrich
  • Article | | open

    The fusion peptide (FP) of HIV envelope (Env) is critical in the cell entry process. Here, Kumar et al. present crystal structures of B41 SOSIP.664 Env trimer and show the dynamic nature of the FP and proximal region, which likely relates to conformational rearrangements required for membrane fusion.

    • Sonu Kumar
    • , Anita Sarkar
    • , Pavel Pugach
    • , Rogier W. Sanders
    • , John P. Moore
    • , Andrew B. Ward
    •  & Ian A. Wilson
  • Article | | open

    Here, Depledge et al. use nanopore arrays for direct RNA sequencing to profile the HSV-1 transcriptome in productively infected cells. Sequencing of individual RNAs reveals a highly complex viral transcriptome including mRNAs encoding new viral fusion proteins derived by read-through transcription.

    • Daniel P. Depledge
    • , Kalanghad Puthankalam Srinivas
    • , Tomohiko Sadaoka
    • , Devin Bready
    • , Yasuko Mori
    • , Dimitris G. Placantonakis
    • , Ian Mohr
    •  & Angus C. Wilson
  • Article | | open

    Electroencephalography (EEG) allows the measurement of electrical signals associated with brain activity, but it is unclear if EEG can accurately measure subcortical activity. Here, the authors show that source dynamics, reconstructed from scalp EEG, correlate with activity recorded from human thalamus and nucleus accumbens.

    • Martin Seeber
    • , Lucia-Manuela Cantonas
    • , Mauritius Hoevels
    • , Thibaut Sesia
    • , Veerle Visser-Vandewalle
    •  & Christoph M. Michel
  • Article | | open

    Adenovirus based (AdV) vectors are promising platforms for therapeutics and vaccines, but receptor usage of serotypes in clinical development remains unclear. Here, based on crystal structures and modeling, Baker et al. show that HAdV-D26/48 fiber knob protein interacts weakly with CAR but not with CD46 or DSG2.

    • Alexander T. Baker
    • , Alexander Greenshields-Watson
    • , Lynda Coughlan
    • , James A. Davies
    • , Hanni Uusi-Kerttula
    • , David K. Cole
    • , Pierre J. Rizkallah
    •  & Alan L. Parker
  • Article | | open

    Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.

    • Ashot Papikian
    • , Wanlu Liu
    • , Javier Gallego-Bartolomé
    •  & Steven E. Jacobsen
  • Article | | open

    Calcium signalling downstream of VEGF is essential for VEGF-induced angiogenesis. Here Savage et al. show that Transmembrane Protein 33 (TMEM33) is required for angiogenesis and the endothelial calcium response to VEGF, revealing a function for TMEM33 in multicellular organisms.

    • Aaron M. Savage
    • , Sathishkumar Kurusamy
    • , Yan Chen
    • , Zhen Jiang
    • , Karishma Chhabria
    • , Ryan B. MacDonald
    • , Hyejeong R. Kim
    • , Heather L. Wilson
    • , Fredericus J. M. van Eeden
    • , Angel L. Armesilla
    • , Timothy J. A. Chico
    •  & Robert N. Wilkinson
  • Article | | open

    Quaternary contacts mediated by an extended heavy-chain framework region 3 (FR3) have been shown to improve binding to HIV envelope and virus neutralization for a few antibodies. Here, Liu et al. engraft such an FR3 loop onto several potent broadly neutralizing antibodies, resulting in improved neutralization activity and pharmacokinetics.

    • Qingbo Liu
    • , Yen-Ting Lai
    • , Peng Zhang
    • , Mark K. Louder
    • , Amarendra Pegu
    • , Reda Rawi
    • , Mangaiarkarasi Asokan
    • , Xuejun Chen
    • , Chen-Hsiang Shen
    • , Gwo-Yu Chuang
    • , Eun Sung Yang
    • , Huiyi Miao
    • , Yuge Wang
    • , Anthony S. Fauci
    • , Peter D. Kwong
    • , John R. Mascola
    •  & Paolo Lusso
  • Article | | open

    ATP has essential roles in cell signalling and energy homeostasis and biosensors to detect it have many potential applications. Here, the authors develop a new ATP sensor that can be targeted to the membrane or cytosol.

    • Mark A. Lobas
    • , Rongkun Tao
    • , Jun Nagai
    • , Mira T. Kronschläger
    • , Philip M. Borden
    • , Jonathan S. Marvin
    • , Loren L. Looger
    •  & Baljit S. Khakh
  • Article | | open

    No structural data have been available for RNA polymerase holoenzymes or transcription initiation complexes that contain extracytoplasmic σ factors. Here the authors report the crystal structures of transcription initiation complexes comprising Mycobacterium tuberculosis RNA polymerase, extracytoplasmic σ factor σL and promoter DNA.

    • Wei Lin
    • , Sukhendu Mandal
    • , David Degen
    • , Min Sung Cho
    • , Yu Feng
    • , Kalyan Das
    •  & Richard H. Ebright
  • Article | | open

    The antitermination factor RfaH adopts two functional states where its C-terminal domain is folded either as an α-helical hairpin or β-barrel. Here the authors employ solution state NMR measurements to show that the C-terminal domain transforms into the β-barrel only upon binding to the elongation complex and refolds back after dissociation.

    • Philipp Konrad Zuber
    • , Kristian Schweimer
    • , Paul Rösch
    • , Irina Artsimovitch
    •  & Stefan H. Knauer
  • Article | | open

    Transcription is globally repressed in early stage of embryo development, but a set of genes including pri-miR-430 and zinc finger genes is known to escape the repression. Here the authors image the very first transcriptional activities in the living zebra fish embryo, demonstrating a cell cycle-coordinated polymerase II transcription compartment.

    • Yavor Hadzhiev
    • , Haseeb K. Qureshi
    • , Lucy Wheatley
    • , Ledean Cooper
    • , Aleksandra Jasiulewicz
    • , Huy Van Nguyen
    • , Joseph W. Wragg
    • , Divyasree Poovathumkadavil
    • , Sascha Conic
    • , Sarah Bajan
    • , Attila Sik
    • , György Hutvàgner
    • , Làszlò Tora
    • , Agnieszka Gambus
    • , John S. Fossey
    •  & Ferenc Müller
  • Article | | open

    While methane has become an increasingly important chemical feedstock, its selective conversion to other chemicals has proven challenging. Here, authors demonstrate a photocatalyst based on earth-abundant elements that selectively converts methane to carbon monoxide under mild conditions.

    • Xiang Yu
    • , Vincent De Waele
    • , Axel Löfberg
    • , Vitaly Ordomsky
    •  & Andrei Y. Khodakov
  • Article | | open

    The search for peroxidase-like as well as other enzyme-like nanozymes mainly relies on trial-and-error strategies, due to the lack of predictive descriptors. Here, the authors fill this gap by investigating the occupancy of eg orbitals as a possible descriptor for the peroxidase-like activity of transition metal oxide nanozymes

    • Xiaoyu Wang
    • , Xuejiao J. Gao
    • , Li Qin
    • , Changda Wang
    • , Li Song
    • , Yong-Ning Zhou
    • , Guoyin Zhu
    • , Wen Cao
    • , Shichao Lin
    • , Liqi Zhou
    • , Kang Wang
    • , Huigang Zhang
    • , Zhong Jin
    • , Peng Wang
    • , Xingfa Gao
    •  & Hui Wei
  • Article | | open

    While the conversion of CO2 to valuable, storable chemicals is attractive, there are few inexpensive and abundant catalysts that are also active and selective for liquid fuels. Here, the authors study copper selenide as a high-performing and efficient electrocatalyst for CO2 conversion to methanol.

    • Dexin Yang
    • , Qinggong Zhu
    • , Chunjun Chen
    • , Huizhen Liu
    • , Zhimin Liu
    • , Zhijuan Zhao
    • , Xiaoyu Zhang
    • , Shoujie Liu
    •  & Buxing Han
  • Article | | open

    Electrochromic technology has diverse cutting-edge applications, but it has never been used to overcome the critical problems in the field of surface-enhanced Raman scattering (SERS). Here, the authors demonstrate a generic electrochromic strategy for ensuring the reproducibility and renewability of SERS substrates.

    • Shan Cong
    • , Zhen Wang
    • , Wenbin Gong
    • , Zhigang Chen
    • , Weibang Lu
    • , John R. Lombardi
    •  & Zhigang Zhao
  • Article | | open

    Many 2-Cystein Peroxiredoxins (Prx) can either function as peroxidases or chaperones when exposed to stress. Here the authors present the structures of Leishmania infantum mitochondrial Prx alone and with a bound model client protein, use crosslinking to reveal interaction regions that stabilize the bound client, and provide insights into the mechanism by which Prx’s adopt chaperone activity.

    • Filipa Teixeira
    • , Eric Tse
    • , Helena Castro
    • , Karl A. T. Makepeace
    • , Ben A. Meinen
    • , Christoph H. Borchers
    • , Leslie B. Poole
    • , James C. Bardwell
    • , Ana M. Tomás
    • , Daniel R. Southworth
    •  & Ursula Jakob
  • Article | | open

    Here, the authors integrate an acoustic trap with a compact light sheet fluorescence microscope to provide contactless sample confinement of biological organisms. The application of verapamil and norepinephrine to zebrafish larvae was also studied, and the heartbeat response was monitored with light sheet imaging.

    • Zhengyi Yang
    • , Katy L. H. Cole
    • , Yongqiang Qiu
    • , Ildikó M. L. Somorjai
    • , Philip Wijesinghe
    • , Jonathan Nylk
    • , Sandy Cochran
    • , Gabriel C. Spalding
    • , David A. Lyons
    •  & Kishan Dholakia
  • Article | | open

    BMP ligands act as retrograde signalling molecules to regulate presynaptic development, and regulation of BMP receptors by endocytosis may be an important component of this signalling pathway. Here, the authors show that Abi-mediated macropinocytosis of BMP receptors in Drosophila larva and contributes to neuromuscular development.

    • Najin Kim
    • , Sungdae Kim
    • , Minyeop Nahm
    • , Danielle Kopke
    • , Joohyung Kim
    • , Eunsang Cho
    • , Min-Jung Lee
    • , Mihye Lee
    • , Seung Hyun Kim
    • , Kendal Broadie
    •  & Seungbok Lee
  • Article | | open

    Highly ordered and compositionally complex ceramics are prone to disordering under irradiation, but exactly how is unclear. Here, the authors use high resolution microscopy to directly image the order-to-disorder phase transformations in Ti3AlC2 into otherwise unattainable solid solutions.

    • Chenxu Wang
    • , Tengfei Yang
    • , Cameron L. Tracy
    • , Chenyang Lu
    • , Hui Zhang
    • , Yong-Jie Hu
    • , Lumin Wang
    • , Liang Qi
    • , Lin Gu
    • , Qing Huang
    • , Jie Zhang
    • , Jingyang Wang
    • , Jianming Xue
    • , Rodney C. Ewing
    •  & Yugang Wang
  • Article | | open

    Many Gram-negative bacteria rely on a type III secretion system (T3SS) for their pathogenicity. Here authors present the cryo-EM structure of the E.coli T3SS ATPase-central stalk complex, which forms a homohexameric, asymmetric pore with different functional states.

    • Dorothy D. Majewski
    • , Liam J. Worrall
    • , Chuan Hong
    • , Claire E. Atkinson
    • , Marija Vuckovic
    • , Nobuhiko Watanabe
    • , Zhiheng Yu
    •  & Natalie C. J. Strynadka
  • Article | | open

    Place cells and grid cells are known to encode spatial information about an animal’s location relative to the surrounding environment. Here, the authors show that place cells predominantly encode environmental sensory inputs, while grid cell activity reflects a greater influence of physical motion.

    • Guifen Chen
    • , Yi Lu
    • , John A King
    • , Francesca Cacucci
    •  & Neil Burgess
  • Article | | open

    Elp3 is the catalytic subunit of the eukaryotic Elongator complex that catalyzes posttranscriptional tRNA modifications. Here the authors present the crystal structures of an acetyl-CoA analog bound bacterial Elp3 and a monomeric archaeal Elp3 and show that Elp3 functions as a tRNA modification enzyme in all domains of life.

    • Ting-Yu Lin
    • , Nour El Hana Abbassi
    • , Karol Zakrzewski
    • , Andrzej Chramiec-Głąbik
    • , Małgorzata Jemioła-Rzemińska
    • , Jan Różycki
    •  & Sebastian Glatt
  • Article | | open

    The FDA approved drug aprepitant is an antagonist of the Neurokinin 1 receptor (NK1R). Here the authors present aprepitant bound NK1R crystal structures and use NMR spectroscopy to gain further insights into the dynamics of aprepitant binding, which is of interest for further drug development.

    • Shuanghong Chen
    • , Mengjie Lu
    • , Dongsheng Liu
    • , Lingyun Yang
    • , Cuiying Yi
    • , Limin Ma
    • , Hui Zhang
    • , Qing Liu
    • , Thomas M. Frimurer
    • , Ming-Wei Wang
    • , Thue W. Schwartz
    • , Raymond C. Stevens
    • , Beili Wu
    • , Kurt Wüthrich
    •  & Qiang Zhao
  • Article | | open

    Development of bacterial resistance to an antibiotic can lead to collateral sensitivity to another drug. Here, the authors study collateral sensitivity conferred by mutations in the horizontally acquired β-lactamase CTX-M-15, and identify antibiotic combinations that constrain the evolution of resistance.

    • Carola E. H. Rosenkilde
    • , Christian Munck
    • , Andreas Porse
    • , Marius Linkevicius
    • , Dan I. Andersson
    •  & Morten O. A. Sommer
  • Article | | open

    Zinc ions (Zn2+) are imported by Golgi-resident transporters but the function of zinc in the early secretory pathway has remained unknown. Here the authors find that Zn2+ regulates protein quality control in the early secretory pathway by demonstrating that the pH-sensitive chaperone ERp44 binds Zn2+ and solving the Zn2+-bound ERp44 structure.

    • Satoshi Watanabe
    • , Yuta Amagai
    • , Sara Sannino
    • , Tiziana Tempio
    • , Tiziana Anelli
    • , Manami Harayama
    • , Shoji Masui
    • , Ilaria Sorrentino
    • , Momo Yamada
    • , Roberto Sitia
    •  & Kenji Inaba
  • Article | | open

    End-product toxicity, culture contamination, and energy efficient product recovery are long-standing issues in bioprocessing. Here, the authors address these problems using a fermentation strategy that combines microbial production of branched alcohols with supercritical carbon dioxide extraction.

    • Jason T. Boock
    • , Adam J. E. Freedman
    • , Geoffrey A. Tompsett
    • , Sarah K. Muse
    • , Audrey J. Allen
    • , Luke A. Jackson
    • , Bernardo Castro-Dominguez
    • , Michael T. Timko
    • , Kristala L. J. Prather
    •  & Janelle R. Thompson
  • Article | | open

    Kinetochore function depends on H4K20 monomethylation in centromeric nucleosomes but the underlying mechanism is unclear. Here, the authors provide evidence that the centromere-specific nucleosome subunit CENP-A facilitates H4K20 methylation by enabling a conformational change of the H4 N-terminal tail.

    • Yasuhiro Arimura
    • , Hiroaki Tachiwana
    • , Hiroki Takagi
    • , Tetsuya Hori
    • , Hiroshi Kimura
    • , Tatsuo Fukagawa
    •  & Hitoshi Kurumizaka
  • Article | | open

    The TERB1-TERB2-MAJIN complex mediates the attachment of telomeres to the nuclear envelope. Here the authors present the crystal structures of the human TERB1-TERB2 and TERB2-MAJIN subcomplexes and show that Terb2 mutations, which abolish complex formation cause aberrant homologous pairing and disordered synapsis in mouse.

    • Yan Wang
    • , Yanyan Chen
    • , Juan Chen
    • , Lijun Wang
    • , Leitong Nie
    • , Juanjuan Long
    • , Haishuang Chang
    • , Jian Wu
    • , Chenhui Huang
    •  & Ming Lei
  • Article | | open

    The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.

    • Dacheng Ma
    • , Zhimeng Xu
    • , Zhaoyu Zhang
    • , Xi Chen
    • , Xiangzhi Zeng
    • , Yiyang Zhang
    • , Tingyue Deng
    • , Mengfei Ren
    • , Zheng Sun
    • , Rui Jiang
    •  & Zhen Xie
  • Article | | open

    Self-assembly of carbohydrates play an integral part in the design of higher ordered structures, but is limited to amphiphiles where the carbohydrate is covalently bound to a hydrophobic tail. Here the authors show that sugars direct the self-assembly of insoluble curcumin and the formation of well-defined capsules.

    • Sandy Wong
    • , Jiacheng Zhao
    • , Cheng Cao
    • , Chin Ken Wong
    • , Rhiannon P. Kuchel
    • , Sergio De Luca
    • , James M. Hook
    • , Christopher J. Garvey
    • , Sean Smith
    • , Junming Ho
    •  & Martina H. Stenzel
  • Article | | open

    The Caucasus mountain range has impacted on the culture and genetics of the wider region. Here, the authors generate genome-wide SNP data for 45 Eneolithic and Bronze Age individuals across the Caucasus, and find distinct genetic clusters between mountain and steppe zones as well as occasional gene-flow.

    • Chuan-Chao Wang
    • , Sabine Reinhold
    • , Alexey Kalmykov
    • , Antje Wissgott
    • , Guido Brandt
    • , Choongwon Jeong
    • , Olivia Cheronet
    • , Matthew Ferry
    • , Eadaoin Harney
    • , Denise Keating
    • , Swapan Mallick
    • , Nadin Rohland
    • , Kristin Stewardson
    • , Anatoly R. Kantorovich
    • , Vladimir E. Maslov
    • , Vladimira G. Petrenko
    • , Vladimir R. Erlikh
    • , Biaslan Ch. Atabiev
    • , Rabadan G. Magomedov
    • , Philipp L. Kohl
    • , Kurt W. Alt
    • , Sandra L. Pichler
    • , Claudia Gerling
    • , Harald Meller
    • , Benik Vardanyan
    • , Larisa Yeganyan
    • , Alexey D. Rezepkin
    • , Dirk Mariaschk
    • , Natalia Berezina
    • , Julia Gresky
    • , Katharina Fuchs
    • , Corina Knipper
    • , Stephan Schiffels
    • , Elena Balanovska
    • , Oleg Balanovsky
    • , Iain Mathieson
    • , Thomas Higham
    • , Yakov B. Berezin
    • , Alexandra Buzhilova
    • , Viktor Trifonov
    • , Ron Pinhasi
    • , Andrej B. Belinskij
    • , David Reich
    • , Svend Hansen
    • , Johannes Krause
    •  & Wolfgang Haak
  • Article | | open

    Producing individual cannabinoids by metabolically engineered microbes has proven challenging. Here, the authors develop a cell-free enzymatic prenylating system to generate isoprenyl pyrophosphate substrates directly from glucose and produce both common and rare cannabinoids at >1 g/L.

    • Meaghan A. Valliere
    • , Tyler P. Korman
    • , Nicholas B. Woodall
    • , Gregory A. Khitrov
    • , Robert E. Taylor
    • , David Baker
    •  & James U. Bowie
  • Article | | open

    Ribosome-associated quality control (RQC) pathways monitor and respond to stalling of the translating ribosome. Here the authors show that the ribosome associated RQC factor Hel2/ZNF598, an E3 ubiquitin ligase, generally interacts with mRNAs in the vicinity of stop codons.

    • Marie-Luise Winz
    • , Lauri Peil
    • , Tomasz W. Turowski
    • , Juri Rappsilber
    •  & David Tollervey
  • Article | | open

    Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for in vivo imaging.

    • Jes Dreier
    • , Marco Castello
    • , Giovanna Coceano
    • , Rodrigo Cáceres
    • , Julie Plastino
    • , Giuseppe Vicidomini
    •  & Ilaria Testa
  • Article | | open

    Individuals show large variability in their capacity to lose weight and maintain this weight. Here, the authors perform GWAS in two weight loss intervention cohorts and identify two genetic loci associated with weight loss that are taken forward for Bayesian fine-mapping and functional assessment in flies.

    • Armand Valsesia
    • , Qiao-Ping Wang
    • , Nele Gheldof
    • , Jérôme Carayol
    • , Hélène Ruffieux
    • , Teleri Clark
    • , Victoria Shenton
    • , Lisa J. Oyston
    • , Gregory Lefebvre
    • , Sylviane Metairon
    • , Christian Chabert
    • , Ondine Walter
    • , Polina Mironova
    • , Paulina Lau
    • , Patrick Descombes
    • , Nathalie Viguerie
    • , Dominique Langin
    • , Mary-Ellen Harper
    • , Arne Astrup
    • , Wim H. Saris
    • , Robert Dent
    • , Greg G. Neely
    •  & Jörg Hager
  • Article | | open

    The role of mesencephalic astrocyte-derived neurotrophic factor (MANF) in maintenance of protein folding homeostasis inside the ER has remained unclear. Here the authors determine the structure of the complex between MANF and the ER-localized chaperone BiP and provide evidence that MANF serves as an anti-nucleotide exchange factor for BiP.

    • Yahui Yan
    • , Claudia Rato
    • , Lukas Rohland
    • , Steffen Preissler
    •  & David Ron