Biological fluorescence articles from across Nature Portfolio

Reversible non-covalent interaction between a bacterial phytochrome-derived fluorescent protein and its biliverdin chromophore enables near-infrared localization nanoscopy in mammalian cells.

Trait correlations impact evolvability as selection on one trait can influence others. Here, the authors examine trait correlation in two proteins, a fluorescent protein & an antibiotic resistance enzyme, observing rapid evolution of trait correlations through changes in the biophysical properties of these proteins.

The jellyfish-derived green fluorescent protein StayGold is bright and hardly fades, contributing to improving spatiotemporal resolution and dramatically extending the observation period. To fully benefit from the rich photon budget, we tried some unusual illumination modalities for sustainable, quantitative live-cell or volumetric imaging.

A combination of Bayesian inference, physics modeling, and Markov chain Monte Carlo sampling allows for accurate inference of biomolecule numbers and their photophysical state in cellular clusters.

Webb’s work helped fundamentally reshape basic research and advanced manufacturing in the generation and application of photonics across disciplines, from fundamental and applied physics to the biosciences.