Bacterial structural biology articles from across Nature Portfolio

Using Alpha fold modelling and cryo-EM reconstruction the authors reveal the structural and architectural principles of the bacterial functional amyloid curli, encompassing the continuous stacking of β-solenoid pseudo repeats within and across subunits.

The cryogenic electron microscopy structure of the Pseudomonas aeruginosa cell division complex and antibiotic drug target, FtsWIQBL, suggests a possible activation mechanism.

Here the authors report the crystal structures of RNase J from Mycobacterium tuberculosis in apo-state and complex with single strand RNA, revealing its physiological function in RNA metabolism.

During infection, the Legionella effector Lem3 removes a phosphocholine moiety from the human protein Rab1. Here, the authors present the crystal structure of the stabilised Lem3:Rab1b complex, revealing the catalytic mechanism and substrate recognition of PPM phosphatases shaped Lem3.

Structural and biochemical characterization reveals that anhydrotetracycline’s mode of binding and inhibition of type 1 tetracycline destructases (TDases) is distinct from type 2 TDases.

Using cryo-EM, the authors identified 42 MIPs, including outer junction protein CFAP77 and outer dense fibers, in native doublet microtubules of Tetrahymena thermophila. Knockout of CFAP77 reduced ciliary beat frequency and led to outer junction damage.