Bacterial adhesion

Nucleotide second messengers, such as cAMP and c-di-GMP, regulate many physiological processes in bacteria, including biofilm formation. Here, the authors provide evidence of cross-talk between cAMP and c-di-GMP pathways through direct interaction of their effectors, showing that the cAMP receptor protein (CRP) can play regulatory roles at the post-translational level.

Bacterial surface adhesion proteins are characterized by unusual mechanical properties. Here, the authors use atomic force microscopy-based technique to study a surface-anchoring protein Cpe0147 from Clostridium perfringens and show that an ester bond can withstand considerable mechanical forces and prevent complete protein unfolding.

Yu Zeng and Bin Liu investigate the potential biological function of aggregate formations, called rosettes, of the motile and sessile forms of the aquatic bacteria Caulobacter crescentus. Through microscopy studies and computational simulations, they show that rosettes actively traverse long distances through the flagellar motors of very few motile cells by inducing a wheel-like rolling when adjacent to a solid-liquid interface.

The amyloid protein TasA is a main component of the extracellular matrix in Bacillus subtilis biofilms. Here the authors show that, in addition to a structural function during biofilm assembly and interactions with plants, TasA contributes to the stabilization of membrane dynamics during stationary phase.