Autophagosomes articles within Nature Communications

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  • Article
    | Open Access

    How membrane morphology is regulated during autophagosome formation remains elusive. Here, authors reveal a mechanism by which the forming autophagosomal membrane expands with a large opening for non-selective sequestration of the cytoplasm.

    • Tetsuya Kotani
    • , Yuji Sakai
    •  & Hitoshi Nakatogawa
  • Article
    | Open Access

    The endoplasmic reticulum protein DFCP1 is found on omegasomes implicated in autophagosome biogenesis, but its function has remained unknown. Here, Nähse et al. show that DFCP1 is an ATPase that mediates selective autophagy by promoting constriction of large omegasomes.

    • Viola Nähse
    • , Camilla Raiborg
    •  & Harald Stenmark
  • Article
    | Open Access

    Autophagy is known to impact CD4+ T cell proliferation but the identity of autophagosomal components remain unclear. Here the authors leverage a transgenic mouse model to assess the autophagosomal compartment and identify interleukin-7 receptor-α as a key cargo in proliferating CD4+ T cells.

    • Dingxi Zhou
    • , Mariana Borsa
    •  & Anna Katharina Simon
  • Article
    | Open Access

    Hypoxia induces mitochondrial clearance and autophagy, although the upstream mechanisms are not well defined. Here, the authors identify that oxygen-sensitive methylation of the key autophagy regulator ULK1 promotes ULK1 activation and subsequent autophagosome formation and mitochondrial clearance.

    • Jingyi Li
    • , Tao Zhang
    •  & Rui Liu
  • Article
    | Open Access

    RIPK1 is critical for normal development and cell death. Here, the authors identify a metabolic role for RIPK1 in aspartate homeostasis, as increased aspartate levels in RIPK1-deficient cells inhibits starvation-induced autophagy by ULK1.

    • Xinyu Mei
    • , Yuan Guo
    •  & Zheng-Jiang Zhu
  • Article
    | Open Access

    The source of phospholipids to generate autophagosomal membranes, particularly after prolonged starvation, is not well characterized. Here, the authors show that CCTβ3, the rate limiting enzyme in phosphatidylcholine synthesis, is activated on lipid droplets and sustains long-term autophagy.

    • Yuta Ogasawara
    • , Jinglei Cheng
    •  & Toyoshi Fujimoto
  • Article
    | Open Access

    Autosomal dominant optic atrophy is caused by mutations in the mitochondrial fusion protein OPA1. Here, the authors show that AMPK-induced autophagy depletes mitochondria in axons of retinal ganglion cells and that autophagic inhibition reverses vision loss in a mouse model.

    • Marta Zaninello
    • , Konstantinos Palikaras
    •  & Luca Scorrano
  • Article
    | Open Access

    Methylmalonic acidemia is an inherited metabolic disease caused by loss or mutation of the enzyme MMUT. Here the authors use cell and animal models to show that MMUT mutations lead to defective mitophagy and stress in kidney cells, contributing to the pathogenesis in methylmalonic acidemia patients.

    • Alessandro Luciani
    • , Anke Schumann
    •  & Olivier Devuyst
  • Article
    | Open Access

    The influenza virus hemagglutinin (HA) protein is a target for antivirals. Here, using a multivalent peptide library screen, the authors identify PVF-tet as an HA inhibitor and show that the peptide inhibits IAV replication by sequestering HA into amphisomes, suggesting these to represent a type of anti-viral machinery.

    • Jumpei Omi
    • , Miho Watanabe-Takahashi
    •  & Kiyotaka Nishikawa
  • Article
    | Open Access

    Arabidopsis EH/Pan1 proteins are part of the TPLATE complex (TPC) that is required for endocytosis in plants. Here, the authors show AtEH/Pan1 proteins also act in actin-mediated autophagy, by interacting with VAP27-1 at ER-PM contact sites and recruiting TPLATE and AP-2 complex subunits, clathrin and ARP2/3/ proteins to autophagosomes.

    • Pengwei Wang
    • , Roman Pleskot
    •  & Patrick J. Hussey
  • Article
    | Open Access

    Autophagic cells coordinate substrate remodeling with sequestration during autophagosome formation. Here, the authors show that during Parkin-mediated mitophagy, mitochondria are disassembled into progressively smaller aggregates near autophagy initiation sites in a PtdIns(4,5)P2-dependent manner.

    • Cheng-Wei Hsieh
    •  & Wei Yuan Yang
  • Article
    | Open Access

    Autophagy is a catabolic process whereby cellular components are degraded by the autophagosome, but the role of the actin cytoskeleton is not clear. Here Coutts and La Thangue show that the actin nucleator JMY is recruited to the autophagosome via binding LC3, and promotes actin nucleation that is required for autophagosome maturation.

    • Amanda S. Coutts
    •  & Nicholas B. La Thangue
  • Article
    | Open Access

    Beclin 1 is an essential autophagy effector, necessary to form the autophagosome. Here Sun et al. show that Beclin 1 acetylation regulated by p300 and SIRT1 inhibits autophagosome maturation, and mutation of the acetylation sites leads to tumour growth suppression in vivo.

    • Ting Sun
    • , Xuan Li
    •  & Xiao-Feng Zhu
  • Article
    | Open Access

    BafilomycinA1 is an autophagy inhibitor, presumably owing to its blocking effect on the lysosomal proton pump V-ATPase. Here the authors show that V-ATPase-deficient lysosomes can still fuse with autophagosomes, showing that lysosomal acidification and fusion are two separable, independent events.

    • Caroline Mauvezin
    • , Péter Nagy
    •  & Thomas P. Neufeld
  • Article |

    Compared with yeast and animal cells, the detailed dynamics of autophagosome extension in plants remain particularly unclear. Here the authors show that the autophagy-related protein, ATG5, forms a torus-shaped domain on the early phagophore in Arabidopsis, and involve the ER in a model for plant autophagosome formation.

    • Romain Le Bars
    • , Jessica Marion
    •  & Michele Wolfe Bianchi
  • Article |

    The core mechanisms of autophagy are generally assumed to be broadly conserved between yeast and mammals. Cheng et al. show that the distribution of PtdIns(3)P between cytoplasmic and luminal leaflets of autophagosomes differs dramatically in yeast and mammalian cells, suggestive of different underlying mechanisms.

    • Jinglei Cheng
    • , Akikazu Fujita
    •  & Toyoshi Fujimoto
  • Article |

    Macroautophagy involves remodelling of intracellular membranes, but the role of lipids and lipid enzymes in this process is poorly understood. Here, the authors demonstrate that phospholipase D1, an enzyme that catalyses the production of phosphatidic acid, has a modulatory role in the autophagy process in mammalian cells.

    • Claudia Dall'Armi
    • , Andrés Hurtado-Lorenzo
    •  & Gilbert Di Paolo