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Assay systems are methods that are used to measure the presence, amount or activity of a substance e.g. a drug, cell type or cell component. A wide range of experimental methods are used to measure different components of organic samples in assay systems.
BRET-based biosensors allow the quantitative live-cell monitoring of RTKmediated signaling, revealing ligand-specific spatiotemporal and mutation-specific signaling biases, advancing the understanding of RTK functional selectivity.
Fusion proteins between roGFP2 and glutaredoxins are used for intracellular redox measurements. Here, the authors determined all rate constants of the reaction cycle for roGFP2 measurements and identified an alternative glutaredoxin mechanism.
A key aim in the development of diagnostic assays is improving diagnostic speed while maintaining sensitivity. Here the authors report an approach for the rapid and accurate analysis of lateral flow tests, which integrates time-series deep learning and AI verification, achieving a diagnostic time of 1-2 minutes.
The discovery of antibodies that bind with high affinity to clinically relevant antigens can be sped up by leveraging next-generation sequencing to screen hundreds of millions of antibody–antigen interactions.
Pharmaceutical companies continue to advocate for the use of in vitro models towards the reduction of animal use in drug discovery and development while acknowledging that further advancements are needed to heighten the models’ current state of readiness.
DNA-based molecular computation allows for the simultaneous detection of multiple types of biomarker, as shown for the accurate identification of prostate cancer in serum samples on the basis of specific RNAs, proteins and small molecules.
INSPECTR is a technique for detecting nucleic acids that couples the sensitivity and specificity of nucleic acid splinted ligation with the versatile readouts of cell-free gene expression. The result is an ambient-temperature workflow that enables the detection of pathogenic viruses at low copy numbers.