Assay systems

  • Article
    | Open Access

    Progress in drug discovery can be hampered by a limited exploration of chemical space and the difficulty in assessing the full range of drug candidates’ effects on living cells. Here the authors describe a cell-based assay to distinguish between off-target and specific effects of candidate compounds targeting micro RNAs.

    • Benjamin Haefliger
    • , Laura Prochazka
    •  & Yaakov Benenson
  • Article
    | Open Access

    Culturing normal primary breast cells that express the oestrogen receptor is difficult. Here, the authors isolate oestrogen receptor positive normal breast cells using the cell surface markers CD166 and CD117, and show that the cultures can be repeatedly passaged and retain oestrogen receptor protein expression.

    • Agla J. Fridriksdottir
    • , Jiyoung Kim
    •  & Lone Rønnov-Jessen
  • Article |

    Bioassays are the standard way to measure prion infectivity titres, but can be time-consuming. In this study, bioassays are compared with a modified version of the protein misfolding cyclic amplification technique with beads (PMCAb), demonstrating that PMCAb can be more precise and faster than bioassays.

    • Natallia Makarava
    • , Regina Savtchenko
    •  & Ilia V. Baskakov
  • Article |

    Protein interactions in biological environments are expected to differ from the situationin vitro. In this study, a thermophoresis-based technique is described that allows the analysis of protein and small-molecule interactions in biological liquids; the work may allow more efficient drug development.

    • Christoph J. Wienken
    • , Philipp Baaske
    •  & Stefan Duhr