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Whole-genome screens reveal regulators of differentiation state and context-dependent migration in human neutrophils
Neutrophils provide a critical early defense as part of our innate immune system. Here, authors performed a genome-wide assessment of the molecular factors critical to proliferation, differentiation, and cell migration in a neutrophil-like cell.
- Nathan M. Belliveau
- , Matthew J. Footer
- & Julie A. Theriot
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Article
| Open Access
AMPK is a mechano-metabolic sensor linking cell adhesion and mitochondrial dynamics to Myosin-dependent cell migration
Cell metabolism must adapt to the energy needs of migrating cells. This study finds that fast amoeboid migrating cells harbor high AMPK activity, which controls both mitochondrial dynamics and cytoskeletal remodeling, enabling reduced energy needs.
- Eva Crosas-Molist
- , Vittoria Graziani
- & Victoria Sanz-Moreno
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The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II
Myosin II–mediated contractility is required for leukocyte migration. Here, authors show that lysosomes are involved in leukocyte migration by providing the platform where Ragulator complex interacts with the myosin phosphatase Rho-interacting protein (MPRIP) independently of mTORC1 and interferes with the interaction between MPRIP and a subunit of myosin light chain phosphatase (MLCP).
- Takeshi Nakatani
- , Kohei Tsujimoto
- & Atsushi Kumanogoh
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Article
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STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
The cytoplasm in mammalian cells is considered homogeneous. Here authors report that the cytoplasmic fluidity is regulated in the blebbing cells, which is regulated by calcium concentration in the expanding blebs and involves the STIM-Orai1 pathway.
- Kana Aoki
- , Shota Harada
- & Junichi Ikenouchi
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Article
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Chemokine receptor trafficking coordinates neutrophil clustering and dispersal at wounds in zebrafish
Inflammatory responses must be induced and resolved timely to serve protection from pathogens without inducing excessive tissue damage. Here the authors use live imaging in zebrafish to show that the intracellular trafficking of two chemokine receptors, Cxcr1 and Cxcr2, is differentially regulated on activated neutrophils to control their clustering and dispersal, respectively.
- Caroline Coombs
- , Antonios Georgantzoglou
- & Milka Sarris
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Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures
Here, using an integrative experimental and computational approach, Imle et al. show how cell motility and density affect HIV cell-associated transmission in a three-dimensional tissue-like culture system of CD4+ T cells and collagen, and how different collagen matrices restrict infection by cell-free virions.
- Andrea Imle
- , Peter Kumberger
- & Oliver T. Fackler
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Revealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis
The lack of an appropriate method has hampered quantitative measurements of cell chirality. Here, the authors combine Riesz transform-differential interference contrast microscopy and computational kinematic analysis to reveal chiral cell motility of neuronal growth cone filopodia and cellular slime mold.
- Atsushi Tamada
- & Michihiro Igarashi
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Diverse matrix metalloproteinase functions regulate cancer amoeboid migration
Migrating cancer cells are round or elongated, and it is thought that the differently shaped cells invade surrounding tissue using different mechanisms. Here, Orgaz et al. show that the round cells secrete matrix metalloproteinases, which allow them to degrade surrounding connective tissue more effectively than elongated cells.
- Jose L. Orgaz
- , Pahini Pandya
- & Victoria Sanz-Moreno
YAP silencing by RB1 mutation is essential for small-cell lung cancer metastasis