Alternative splicing

Alternative splicing is a process that enables a messenger RNA (mRNA) to direct synthesis of different protein variants (isoforms) that may have different cellular functions or properties. It occurs by rearranging the pattern of intron and exon elements that are joined by splicing to alter the mRNA coding sequence.

Latest Research and Reviews

  • Research | | open

    Alternative splicing is a critical step in eukaryotic gene expression but its molecular rules are not fully understood. Here, the authors develop a high-throughput mutagenesis approach to comprehensively characterise determinants of alternative splicing for the RON proto-oncogene.

    • Simon Braun
    • , Mihaela Enculescu
    • , Samarth T. Setty
    • , Mariela Cortés-López
    • , Bernardo P. de Almeida
    • , F. X.  Reymond Sutandy
    • , Laura Schulz
    • , Anke Busch
    • , Markus Seiler
    • , Stefanie Ebersberger
    • , Nuno L. Barbosa-Morais
    • , Stefan Legewie
    • , Julian König
    •  & Kathi Zarnack
  • Research | | open

    Cytoplasmic PTEN is a tumor suppressor that antagonises PI3K signalling. Here, the authors show that nuclear PTEN can interact with the spliceosomal proteins and drive pre-mRNA splicing in a phosphatase-independent manner, in particular, PTEN depletion promotes Golgi extension and secretion through GOLGA2 exon skipping.

    • Shao-Ming Shen
    • , Yan Ji
    • , Cheng Zhang
    • , Shuang-Shu Dong
    • , Shuo Yang
    • , Zhong Xiong
    • , Meng-Kai Ge
    • , Yun Yu
    • , Li Xia
    • , Meng Guo
    • , Jin-Ke Cheng
    • , Jun-Ling Liu
    • , Jian-Xiu Yu
    •  & Guo-Qiang Chen
  • Research | | open

    Alternative splicing of influenza A virus (IAV) M transcript is regulated by hnRNP K and NS1-BP, but mechanistic details are unknown. Here, Thompson et al. show how hnRNP K and NS1-BP bind M mRNA and that these proteins regulate splicing of host transcripts in both the absence and presence of IAV infection.

    • Matthew G. Thompson
    • , Raquel Muñoz-Moreno
    • , Prasanna Bhat
    • , Renat Roytenberg
    • , John Lindberg
    • , Matthew R. Gazzara
    • , Michael J. Mallory
    • , Ke Zhang
    • , Adolfo García-Sastre
    • , Beatriz M. A. Fontoura
    •  & Kristen W. Lynch
  • Research | | open

    The precise timing of neurodevelopmental splicing switches and the underlying regulatory mechanisms remain poorly understood. This study identifies two major waves of developmental switches under the control of distinct combinations of RNA-binding proteins in central and peripheral nervous systems.

    • Sebastien M. Weyn-Vanhentenryck
    • , Huijuan Feng
    • , Dmytro Ustianenko
    • , Rachel Duffié
    • , Qinghong Yan
    • , Martin Jacko
    • , Jose C. Martinez
    • , Marianne Goodwin
    • , Xuegong Zhang
    • , Ulrich Hengst
    • , Stavros Lomvardas
    • , Maurice S. Swanson
    •  & Chaolin Zhang

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