nature.com search

Loading Filters

Showing 1–50 of 55 results

Loading Filters

Correspondence | 12 October 2020
Animal-free alternatives and the antibody iceberg
Nature Biotechnology 38 , 1234–1239

Rights & permissionsfor article Animal-free alternatives and the antibody iceberg . Opens in a new window.
Correspondence | 15 May 2020
Reproducibility: bypass animals for antibody production
Nature 581 , 262–262

Rights & permissionsfor article Reproducibility: bypass animals for antibody production . Opens in a new window.
Correspondence | 27 July 2020
Animal-derived-antibody generation faces strict reform in accordance with European Union policy on animal use
Nature Methods 17 , 755–756

Rights & permissionsfor article Animal-derived-antibody generation faces strict reform in accordance with European Union policy on animal use . Opens in a new window.
Research | 15 October 2020 | Open
Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins
- Kathrin Nowak
- , Florian Rosenthal
- , Tobias Karlberg
- , Mareike Bütepage
- , Ann-Gerd Thorsell
- , Birgit Dreier
- , Jonas Grossmann
- , Jens Sobek
- , Ralph Imhof
- , Bernhard Lüscher
- , Herwig Schüler
- , Andreas Plückthun
- , Deena M. Leslie Pedrioli
- & Michael O. Hottiger
Nature Communications 11 , 1–14

Rights & permissionsfor article Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins . Opens in a new window.
Correspondence | 15 April 2015
Antibodies: validate recombinants once
- Andrew M. Bradbury
- & Andreas Plückthun
Nature 520 , 295–295

Rights & permissionsfor article Antibodies: validate recombinants once . Opens in a new window.
Research | 7 November 2019 | Open
Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts
Scientific Reports 9 , 1–10

Rights & permissionsfor article Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts . Opens in a new window.
Research | 3 July 2020 | Open
Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol
Communications Biology 3 , 1–11

Rights & permissionsfor article <i>Salmonella</i>-based platform for efficient delivery of functional binding proteins to the cytosol . Opens in a new window.
Research | 3 January 2017 | Open
Personalised proteome analysis by means of protein microarrays made from individual patient samples
Scientific Reports 7 , 1–7

Rights & permissionsfor article Personalised proteome analysis by means of protein microarrays made from individual patient samples . Opens in a new window.
Research | 18 February 2016 | Open
A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution
Scientific Reports 6 , 1–9

Rights & permissionsfor article A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution . Opens in a new window.
Research | 5 March 2020 | Open
Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation
- Martina Petrenčáková
- , František Filandr
- , Andrej Hovan
- , Ghazaleh Yassaghi
- , Petr Man
- , Tibor Kožár
- , Marc-Simon Schwer
- , Daniel Jancura
- , Andreas Plückthun
- , Petr Novák
- , Pavol Miškovský
- , Gregor Bánó
- & Erik Sedlák
Scientific Reports 10 , 1–15

Rights & permissionsfor article Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation . Opens in a new window.
Comments and Opinion | 4 February 2015
Reproducibility: Standardize antibodies used in research
- ...proteins, say Andrew Bradbury, Andreas Plückthun and 110 co-signatories.
- Andrew Bradbury
- & Andreas Plückthun
Nature 518 , 27–29

Rights & permissionsfor article Reproducibility: Standardize antibodies used in research . Opens in a new window.
Research | 17 March 1994
Destabilization of the complete protein secondary structure on binding to the chaperone GroEL
Nature 368 , 261–265

Rights & permissionsfor article Destabilization of the complete protein secondary structure on binding to the chaperone GroEL . Opens in a new window.
1 June 1997
Selectively infective phage (SIP) technology: A novel method for in vivo selection of interacting protein–ligand pairs
- Stefania Spada
- & Andreas Plückthun
Nature Medicine 3 , 694–696

Rights & permissionsfor article Selectively infective phage (SIP) technology: A novel method for <i>in vivo</i> selection of interacting protein–ligand pairs . Opens in a new window.
Amendments and Corrections | 1 February 2007
Correction: Corrigendum: ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome
- Michael J Taussig
- , Oda Stoevesandt
- , Carl A K Borrebaeck
- , Andrew R Bradbury
- , Dolores Cahill
- , Christian Cambillau
- , Antoine de Daruvar
- , Stefan Dübel
- , Jutta Eichler
- , Ronald Frank
- , Toby J Gibson
- , David Gloriam
- , Larry Gold
- , Friedrich W Herberg
- , Henning Hermjakob
- , Jörg D Hoheisel
- , Thomas O Joos
- , Olli Kallioniemi
- , Manfred Koegl
- , Zoltán Konthur
- , Bernhard Korn
- , Elisabeth Kremmer
- , Sylvia Krobitsch
- , Ulf Landegren
- , Silvère van der Maarel
- , John McCafferty
- , Serge Muyldermans
- , Per-Åke Nygren
- , Sandrine Palcy
- , Andreas Plückthun
- , Bojan Polic
- , Michael Przybylski
- , Petri Saviranta
- , Alan Sawyer
- , David J Sherman
- , Arne Skerra
- , Markus Templin
- , Marius Ueffing
- & Mathias Uhlén
Nature Methods 4 , 187–187

Rights & permissionsfor article Correction: Corrigendum: ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome . Opens in a new window.
Research | 1 December 2000
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
Nature Biotechnology 18 , 1287–1292

Rights & permissionsfor article Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display . Opens in a new window.
1 April 1998
Selection for a periplasmic factor improving phage display and functional periplasmic expression
- Hendrick Bothmann
- & Andreas Plückthun
Nature Biotechnology 16 , 376–380

Rights & permissionsfor article Selection for a periplasmic factor improving phage display and functional periplasmic expression . Opens in a new window.
21 May 2010
Designed ankyrin repeat proteins: a novel tool for testing epidermal growth factor receptor 2 expression in breast cancer
Modern Pathology 23 , 1289–1297

Rights & permissionsfor article Designed ankyrin repeat proteins: a novel tool for testing epidermal growth factor receptor 2 expression in breast cancer . Opens in a new window.
Research | 1 November 1993
Improved Bivalent Miniantibodies, with Identical Avidity as Whole Antibodies, Produced by High Cell Density Fermentation of Escherichia coli
- Peter Pack
- , Marian Kujau
- , Volker Schroeckh
- , Uwe Knüpfer
- , Rolf Wenderoth
- , Dieter Riesenberg
- & Andreas Plückthun
Nature Biotechnology 11 , 1271–1277

Rights & permissionsfor article Improved Bivalent Miniantibodies, with Identical Avidity as Whole Antibodies, Produced by High Cell Density Fermentation of <i>Escherichia coli</i> . Opens in a new window.
Protocols | 27 February 2007
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target
Nature Methods 4 , 269–279

Rights & permissionsfor article Ribosome display: selecting and evolving proteins <i>in vitro</i> that specifically bind to a target . Opens in a new window.
News and Views | 29 September 2010
Antibodies with a split personality
- Andreas Plückthun
Nature 467 , 537–538

Rights & permissionsfor article Antibodies with a split personality . Opens in a new window.
1 September 1999
Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)–carbonyl complex
- Robert Waibel
- , Roger Alberto
- , Jörg Willuda
- , Ricarda Finnern
- , Roger Schibli
- , Albert Stichelberger
- , André Egli
- , Ulrich Abram
- , Jean-Pierre Mach
- , Andreas Plückthun
- & P. August Schubiger
Nature Biotechnology 17 , 897–901

Rights & permissionsfor article Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)–carbonyl complex . Opens in a new window.
1 January 2007
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome
- Michael J Taussig
- , Oda Stoevesandt
- , Carl A K Borrebaeck
- , Andrew R Bradbury
- , Dolores Cahill
- , Christian Cambillau
- , Antoine de Daruvar
- , Stefan Dübel
- , Jutta Eichler
- , Ronald Frank
- , Toby J Gibson
- , David Gloriam
- , Larry Gold
- , Friedrich W Herberg
- , Henning Hermjakob
- , Jörg D Hoheisel
- , Thomas O Joos
- , Olli Kallioniemi
- , Manfred Koegl
- , Zoltán Konthur
- , Bernhard Korn
- , Elisabeth Kremmer
- , Sylvia Krobitsch
- , Ulf Landegren
- , Silvère van der Maarel
- , John McCafferty
- , Serge Muyldermans
- , Per-Åke Nygren
- , Sandrine Palcy
- , Andreas Plückthun
- , Bojan Polic
- , Michael Przybylski
- , Petri Saviranta
- , Alan Sawyer
- , David J Sherman
- , Arne Skerra
- , Markus Templin
- , Marius Ueffing
- & Mathias Uhlén
Nature Methods 4 , 13–17

Rights & permissionsfor article ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome . Opens in a new window.
Research | 23 October 2019 | Open
Structural analysis of biological targets by host:guest crystal lattice engineering
Scientific Reports 9 , 1–11

Rights & permissionsfor article Structural analysis of biological targets by host:guest crystal lattice engineering . Opens in a new window.
Research | 23 April 2019 | Open
A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins
Nature Communications 10 , 1–10

Rights & permissionsfor article A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins . Opens in a new window.
Research | 3 January 2019 | Open
Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists
Nature Communications 10 , 1–11

Rights & permissionsfor article Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists . Opens in a new window.
Research | 21 February 2019 | Open
Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in Escherichia coli
Scientific Reports 9 , 1–14

Rights & permissionsfor article Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in <i>Escherichia coli</i> . Opens in a new window.
Research | 19 November 2018
High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist
Nature Structural & Molecular Biology 25 , 1086–1092

Rights & permissionsfor article High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist . Opens in a new window.
Research | 31 January 2018 | Open
Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control
- Markus Schmid
- , Patrick Ernst
- , Annemarie Honegger
- , Maarit Suomalainen
- , Martina Zimmermann
- , Lukas Braun
- , Sarah Stauffer
- , Cristian Thom
- , Birgit Dreier
- , Matthias Eibauer
- , Anja Kipar
- , Viola Vogel
- , Urs F. Greber
- , Ohad Medalia
- & Andreas Plückthun
Nature Communications 9 , 1–16

Rights & permissionsfor article Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control . Opens in a new window.
Research | 11 July 2018
PtdIns(4,5)P₂ stabilizes active states of GPCRs and enhances selectivity of G-protein coupling
- Hsin-Yung Yen
- , Kin Kuan Hoi
- , Idlir Liko
- , George Hedger
- , Michael R. Horrell
- , Wanling Song
- , Di Wu
- , Philipp Heine
- , Tony Warne
- , Yang Lee
- , Byron Carpenter
- , Andreas Plückthun
- , Christopher G. Tate
- , Mark S. P. Sansom
- & Carol V. Robinson
Nature 559 , 423–427

Rights & permissionsfor article PtdIns(4,5)P<sub>2</sub> stabilizes active states of GPCRs and enhances selectivity of G-protein coupling . Opens in a new window.
Research | 16 October 2017 | Open
A quantitative comparison of cytosolic delivery via different protein uptake systems
Scientific Reports 7 , 1–13

Rights & permissionsfor article A quantitative comparison of cytosolic delivery via different protein uptake systems . Opens in a new window.
Research | 29 June 2017 | Open
Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation
Nature Communications 8 , 1–15

Rights & permissionsfor article Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation . Opens in a new window.
Research | 11 September 2017 | Open
Rigidly connected multispecific artificial binders with adjustable geometries
Scientific Reports 7 , 1–11

Rights & permissionsfor article Rigidly connected multispecific artificial binders with adjustable geometries . Opens in a new window.
Research | 24 November 2017 | Open
Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity
- Simon Hansen
- , Jakob C. Stüber
- , Patrick Ernst
- , Alexander Koch
- , Daniel Bojar
- , Alexander Batyuk
- & Andreas Plückthun
Scientific Reports 7 , 1–16

Rights & permissionsfor article Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity . Opens in a new window.
Research | 6 July 2016 | Open
Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin
Scientific Reports 6 , 1–14

Rights & permissionsfor article Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin . Opens in a new window.
Research | 21 November 2016
Covalently circularized nanodiscs for studying membrane proteins and viral entry
- Mahmoud L Nasr
- , Diego Baptista
- , Mike Strauss
- , Zhen-Yu J Sun
- , Simina Grigoriu
- , Sonja Huser
- , Andreas Plückthun
- , Franz Hagn
- , Thomas Walz
- , James M Hogle
- & Gerhard Wagner
Nature Methods 14 , 49–52

Rights & permissionsfor article Covalently circularized nanodiscs for studying membrane proteins and viral entry . Opens in a new window.
Research | 3 June 2016 | Open
Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS–p110 crosstalk
Nature Communications 7 , 1–18

Rights & permissionsfor article Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS–p110 crosstalk . Opens in a new window.
Research | 25 February 2016 | Open
Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts
Scientific Reports 6 , 1–16

Rights & permissionsfor article Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts . Opens in a new window.
Research | 10 February 2015
Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors
- Robert C. Münch
- , Anke Muth
- , Alexander Muik
- , Thorsten Friedel
- , Julia Schmatz
- , Birgit Dreier
- , Alexandra Trkola
- , Andreas Plückthun
- , Hildegard Büning
- & Christian J. Buchholz
Nature Communications 6 , 1–9

Rights & permissionsfor article Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors . Opens in a new window.
Research | 6 July 2015
Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells
Nature Methods 12 , 773–779

Rights & permissionsfor article Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells . Opens in a new window.
Research | 21 July 2013
Structure of a kinesin–tubulin complex and implications for kinesin motility
- Benoît Gigant
- , Weiyi Wang
- , Birgit Dreier
- , Qiyang Jiang
- , Ludovic Pecqueur
- , Andreas Plückthun
- , Chunguang Wang
- & Marcel Knossow
Nature Structural & Molecular Biology 20 , 1001–1007

Rights & permissionsfor article Structure of a kinesin–tubulin complex and implications for kinesin motility . Opens in a new window.
1 July 1999
An in vivo library-versus-library selection of optimized protein–protein interactions
Nature Biotechnology 17 , 683–690

Rights & permissionsfor article An in vivo library-versus-library selection of optimized protein–protein interactions . Opens in a new window.
Research | 18 May 2003
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library
Nature Biotechnology 21 , 679–685

Rights & permissionsfor article Turnover-based <i>in vitro</i> selection and evolution of biocatalysts from a fully synthetic antibody library . Opens in a new window.
Reviews | 1 June 1991
Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems
- Andreas Plückthun
Nature Biotechnology 9 , 545–551

Rights & permissionsfor article Antibody Engineering: Advances From the Use of <i>Escherichia coli</i> Expression Systems . Opens in a new window.
Research | 1 January 1993
The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli
Nature Biotechnology 11 , 77–83

Rights & permissionsfor article The Effect of Folding Catalysts on the <i>In Vivo</i> Folding Process of Different Antibody Fragments Expressed in <i>Escherichia coli</i> . Opens in a new window.
1 January 1997
Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones
Nature Biotechnology 15 , 79–84

Rights & permissionsfor article Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones . Opens in a new window.
Reviews | 6 October 2005
Engineering novel binding proteins from nonimmunoglobulin domains
Nature Biotechnology 23 , 1257–1268

Rights & permissionsfor article Engineering novel binding proteins from nonimmunoglobulin domains . Opens in a new window.
1 March 1991
The Functional Expression of Antibody F_v Fragments in Ischhuchia coli: Improved Vectors and a Generally Applicable Purification Technique
Nature Biotechnology 9 , 273–278

Rights & permissionsfor article The Functional Expression of Antibody F<sub>v</sub> Fragments in <i>Ischhuchia coli:</i> Improved Vectors and a Generally Applicable Purification Technique . Opens in a new window.
Research | 18 April 2004
High-affinity binders selected from designed ankyrin repeat protein libraries
Nature Biotechnology 22 , 575–582

Rights & permissionsfor article High-affinity binders selected from designed ankyrin repeat protein libraries . Opens in a new window.
1 August 1998
Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments
Nature Biotechnology 16 , 773–777

Rights & permissionsfor article Increasing the secretory capacity of <i>Saccharomyces cerevisiae</i> for production of single-chain antibody fragments . Opens in a new window.
Research | 2 July 2006
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display
Nature Biotechnology 24 , 823–831

Rights & permissionsfor article Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display . Opens in a new window.

Quick links