The experiment that demonstrated that adenovirus DNA contains split genes.
- Author: Richard J. Roberts ,Phillip A. Sharp
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The experiment that demonstrated that adenovirus DNA contains split genes.
The genetic information in the messenger RNA resides in the DNA as four segments, which are separated by three intervening regions (a, b, and c). In the experimentally produced hybrid between one of the DNA strands and the RNA, the intervening sequences in the DNA strand appear as loops, i.e., the corresponding segments lack counterparts in the RNA. The hybrid could be directly visualized in the electron microscope.
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In 1958, Francis Crick's sequence hypothesis finally provided an answer to the question: what is a gene? Why is this definition now considered overly simplistic?
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