Idealized representation of the hierarchical shotgun sequencing strategy.

Author: IHGSC

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Idealized representation of the hierarchical shotgun sequencing strategy.

A library is constructed by fragmenting the target genome and cloning it into a large fragment cloning vector; here, BAC vectors are used. The genomic DNA fragments represented in the library are then organized into a physical map, and individual BAC clones are selected and sequenced by the random shotgun strategy. Finally, the clone sequences are assembled to reconstruct the sequence of the genome.