Reagents
Methanol (Sigma, cat. no. 34860)
Diluent (10x) (Cell Biolabs, cat. no. 10905)
H2O2 (Sigma, cat. no. 349887)
Bovine Serum Albumin ([BSA] Sigma, cat. no. A4503)
SuperSript One-Step RT-PCR with Platinum Taq (Invitrogen, cat. no. 10928-034)
IllustraTM puRetaq Ready-to-go beads (GE Health, cat. no. 27-9557-01)
TRI Reagent ® (Sigma, cat. no. 9424)
2-Isopropanol, anhydrous, (Sigma, cat. no. 278475)
1-bromo-3-chloropropane (BCP) Phase Separation reagent (MRC, cat no. BP151)
DAB Substrate (25X) (Cell Biolabs, cat. no. 10903)
Dulbeccos Modified Medium ([DMEM] Mediatech, cat. No.10-017-CV)
Fetal bovine serum ([FBS] PPA Laboratories, cat. no.A11-034)
Penicillin-streptomycin (Mediatech, cat. no. 30-002-CI)
UltraPure agarose (Invitrogen, cat. no. 15510-027)
DEPC-treated water (Ambion, cat. no. 9915G)
Distilled water
Buffer
Tris, Acetic Acid, EDTA (TAE) buffer (Mediatech, cat. no. 46-010-CM)
Dulbeccos Phosphate Buffered Saline (DPBS) 1X (Mediatech, cat. No.10-030-CM)
Antibody
Anti-hexon antibody (1000X) (Cell Biolabs, cat. no. 10901)
Secondary antibody horse radish peroxidase (HRP) Conjugate (1000X) (Cell Biolabs, cat. no. 10902)
Cells
Human Embryonic Kidney (HEK) 293 cells (ATCC, cat. no. CCL-243)
Recombinant adenovirus (lot number)
AdC6HIVgag (L1121)
AdC7EGFP (L1074)
AdC7HIVgag (L1111)
AdC7SIVgp160 ( L1213)
AdHu5EGFP (L1093)
AdHu5SIVgp160 ( L1225)
Primers
For list of primers consult "Table Primers":http://www.nature.com/protocolexchange/system/uploads/1947/original/PRIMERStable.doc?1318360888