Volume 29

  • No. 9 September 2022

    Hand-over-hand substrate threading by AAA-ATPases

    Dynamic release of the ribosomal maturation factor Rlp24 from the pre-60S ribosome by the AAA-ATPase Drg1 occurs through hand-over-hand substrate translocation.

    See Prattes, Grishkovskaya et al.

  • No. 8 August 2022

    Tail-anchored membrane proteins

    Characterization of the guided entry of tail-anchored proteins (GET) pathway in Giardia intestinalis provides a model for the insertion of tail-anchored membrane proteins.

    See Fry et al.

  • No. 7 July 2022

    Loop extrusion on supercoiled DNA

    Single-molecule experiments reveal that condensin-induced DNA looping is stimulated by positive supercoils on DNA.

    See Kim et al.

  • No. 6 June 2022

    A brake for the chromatin looping cycle

    Two studies on 3D genome organization find that cohesin acetylation puts a brake on chromatin loop extrusion through recruitment of PDS5.

    See Bastié, Chapard et al.and van Ruiten et al.

  • No. 5 May 2022

    H1 & ssDNA enter a new phase

    Single-molecule imaging and manipulation reveals how linker histone H1 preferentially forms phase-separated droplets with single-stranded nucleic acids rather than with double-stranded DNA and nucleosomes, suggestive of a non-canonical nuclear function.

    See Leicher et al.

  • No. 4 April 2022

    Curare stabilizes desensitized nicotinic acetylcholine receptors

    Cryo-EM structures reveal how the nicotinic acetylcholine receptor is desensitized in response to an agonist, and how the arrow poison curare, extracted from Strychnos toxifera (pictured) and other plants, antagonizes the channel by stabilizing the desensitized state.

    See Rahman et al.

  • No. 3 March 2022

    Oxytocin signaling's cation dependence

    A cryo-EM structure and functional analyses of oxytocin bound to its receptor reveal the structural determinants of the magnesium ion dependence of vasopressin/oxytocin family receptors.

    See Article by Meyerowitz, Robertson et al.

  • No. 2 February 2022

    Piwi-directed heterochromatin

    Panoramix SUMOylation on chromatin connects the piRNA pathway to heterochromatin formation in Drosophila.

    See Article by Andreev, Yu, et al.

  • No. 1 January 2022

    IMPDH1 filament architectures

    A series of cryo-EM structures of human IMPDH1 variants reveal polymorphic filaments, which provides a basis for their role in retinal function and disease.

    See Article by Burrell et al.