Volume 25 Issue 4, April 2018

Volume 25 Issue 4

Cotranslational cuts to mRNA

Using a novel approach, Akron-Seq, Mourelatos and colleagues show that mRNAs are subject to ribothrypsis: repeated, cotranslational, endonucleolytic cuts at the ribosome exit channel.

See Ibrahim et al.

Image: Cover Art by Erin Dewalt. Cover Design: Erin Dewalt.

Obituary

News & Views

  • News & Views |

    Recent developments in transcriptome-wide sequencing technologies have enabled the identification of cellular mRNA decay intermediates. Although canonical mRNA decay has been shown to occur by deadenylation followed by decapping and subsequent exonucleolytic decay from both mRNA ends, a study by Mourelatos and colleagues now defines mRNA fragments that are generated on polysomes by endonucleolytic cleavages phased by the associated ribosome.

    • Tatsuaki Kurosaki
    •  & Lynne E. Maquat

Articles

  • Article |

    A lag in nascent strand DNA methylation contributes to heterogeneous methylation in asynchronous cell populations, but cancer cells and active transcription factor binding sites preserve heterogeneity even after cell cycle arrest.

    • Jocelyn Charlton
    • , Timothy L. Downing
    • , Zachary D. Smith
    • , Hongcang Gu
    • , Kendell Clement
    • , Ramona Pop
    • , Veronika Akopian
    • , Sven Klages
    • , David P. Santos
    • , Alexander M. Tsankov
    • , Bernd Timmermann
    • , Michael J. Ziller
    • , Evangelos Kiskinis
    • , Andreas Gnirke
    •  & Alexander Meissner
  • Article |

    Cryo-EM structures of human ABCG2 bound to synthetic derivatives of inhibitor Ko143 or the multidrug resistance modulator tariquidar together with insight into the structure–activity relationship of the Ko143 scaffold provide a basis for the design of novel ABCG2 inhibitors.

    • Scott M. Jackson
    • , Ioannis Manolaridis
    • , Julia Kowal
    • , Melanie Zechner
    • , Nicholas M. I. Taylor
    • , Manuel Bause
    • , Stefanie Bauer
    • , Ruben Bartholomaeus
    • , Guenther Bernhardt
    • , Burkhard Koenig
    • , Armin Buschauer
    • , Henning Stahlberg
    • , Karl-Heinz Altmann
    •  & Kaspar P. Locher
  • Article |

    Single-molecule FRET analysis of DNA binding and unwinding by two engineered Cas9s reveals that their improved specificity is achieved through lower binding and more transient unwinding of mismatched DNA and lower intrinsic cleavage reaction rates.

    • Digvijay Singh
    • , Yanbo Wang
    • , John Mallon
    • , Olivia Yang
    • , Jingyi Fei
    • , Anustup Poddar
    • , Damon Ceylan
    • , Scott Bailey
    •  & Taekjip Ha

Amendments & Corrections