Volume 22 Issue 7, July 2015

Volume 22 Issue 7

The integral membrane protein stearoyl-CoA desaturase-1 introduces a double bond into stearoyl-CoA during the biosynthesis of unsaturated fatty acids. Crystallographic analysis of human SCD-1 provides atomic details of the interaction with its substrate. Cover design by Erin Dewalt. (pp 581–585)

News and Views

  • News & Views |

    Two new studies reveal mechanistic insights into how neurons control the assembly of SNARE complexes and the rapid fusion of synaptic vesicles. Structural, biophysical and functional experiments are combined to elucidate the roles of two critical regulators: Munc13 and synaptotagmin.

    • Mary Munson

Articles

  • Article |

    Asymmetric selection of single-stranded guide RNAs from double-stranded RNA precursors is determined by Ago2, which detects 5′-nucleotide identity and thermodynamic stability of microRNA duplex ends via its MID domain.

    • Hiroshi I Suzuki
    • , Akihiro Katsura
    • , Takahiko Yasuda
    • , Toshihide Ueno
    • , Hiroyuki Mano
    • , Koichi Sugimoto
    •  & Kohei Miyazono
  • Article |

    The structure of the ligand-free HIV-1–Env trimer allows conformational fixation of Env and generation of an antigen that binds CD4 with high affinity and is recognized by broadly neutralizing antibodies but not poorly neutralizing ones.

    • Young Do Kwon
    • , Marie Pancera
    • , Priyamvada Acharya
    • , Ivelin S Georgiev
    • , Emma T Crooks
    • , Jason Gorman
    • , M Gordon Joyce
    • , Miklos Guttman
    • , Xiaochu Ma
    • , Sandeep Narpala
    • , Cinque Soto
    • , Daniel S Terry
    • , Yongping Yang
    • , Tongqing Zhou
    • , Goran Ahlsen
    • , Robert T Bailer
    • , Michael Chambers
    • , Gwo-Yu Chuang
    • , Nicole A Doria-Rose
    • , Aliaksandr Druz
    • , Mark A Hallen
    • , Adam Harned
    • , Tatsiana Kirys
    • , Mark K Louder
    • , Sijy O'Dell
    • , Gilad Ofek
    • , Keiko Osawa
    • , Madhu Prabhakaran
    • , Mallika Sastry
    • , Guillaume B E Stewart-Jones
    • , Jonathan Stuckey
    • , Paul V Thomas
    • , Tishina Tittley
    • , Constance Williams
    • , Baoshan Zhang
    • , Hong Zhao
    • , Zhou Zhou
    • , Bruce R Donald
    • , Lawrence K Lee
    • , Susan Zolla-Pazner
    • , Ulrich Baxa
    • , Arne Schön
    • , Ernesto Freire
    • , Lawrence Shapiro
    • , Kelly K Lee
    • , James Arthos
    • , James B Munro
    • , Scott C Blanchard
    • , Walther Mothes
    • , James M Binley
    • , Adrian B McDermott
    • , John R Mascola
    •  & Peter D Kwong

    Collection:

  • Article |

    New X-ray crystal structure and immunoanalyses of alanyl aminopeptidase N (AnAPN1), a gut antigen of the Anopheles mosquito vector of Plasmodium falciparum, reveal how AnAPN1-specific antibodies block transmission of the malarial parasite.

    • Sarah C Atkinson
    • , Jennifer S Armistead
    • , Derrick K Mathias
    • , Maurice M Sandeu
    • , Dingyin Tao
    • , Nahid Borhani-Dizaji
    • , Brian B Tarimo
    • , Isabelle Morlais
    • , Rhoel R Dinglasan
    •  & Natalie A Borg
  • Article |

    Crystallographic and functional studies reveal the arch-shaped architecture of the Munc13 MUN domain and show the molecular basis for Munc13's role in synaptic-vesicle priming by mediating syntaxin-1 opening and SNARE-complex assembly.

    • Xiaoyu Yang
    • , Shen Wang
    • , Yi Sheng
    • , Mingshu Zhang
    • , Wenjuan Zou
    • , Lijie Wu
    • , Lijun Kang
    • , Josep Rizo
    • , Rongguang Zhang
    • , Tao Xu
    •  & Cong Ma
  • Article |

    The NMR-derived structural model of the dynamic interaction between synaptotagmin-1 and the SNARE complex contributes to addressing the longstanding problem of how synaptotagmin-1 triggers neurotransmitter release.

    • Kyle D Brewer
    • , Taulant Bacaj
    • , Andrea Cavalli
    • , Carlo Camilloni
    • , James D Swarbrick
    • , Jin Liu
    • , Amy Zhou
    • , Peng Zhou
    • , Nicholas Barlow
    • , Junjie Xu
    • , Alpay B Seven
    • , Eric A Prinslow
    • , Rashmi Voleti
    • , Daniel Häussinger
    • , Alexandre M J J Bonvin
    • , Diana R Tomchick
    • , Michele Vendruscolo
    • , Bim Graham
    • , Thomas C Südhof
    •  & Josep Rizo
  • Article |

    Bacterial energy-coupling factor (ECF) transporters mediate ATP-dependent uptake of essential environmental micronutrients. Biochemical and fluorescence analyses now show that ATP binding promotes release of a substrate-capturing subunit that dynamically reassociates with the transmembrane module during the transport cycle.

    • Nathan K Karpowich
    • , Jin Mei Song
    • , Nicolette Cocco
    •  & Da-Neng Wang