Volume 22 Issue 10, October 2015

Volume 22 Issue 10

Stabilized capsids of foot-andmouth disease virus, engineered by strengthening protein-protein interfaces, generate improved antibody responses in calves and guinea pigs.Cover image by © Tetiana Vitsenko/Alamy Stock Photo (pp 788–794)

Editorial

  • Editorial |

    Sharing source data—the actual measurements and unprocessed images behind the graphical representations used in figures—helps to ensure transparency and reproducibility of research results. We urge our authors to submit and share the source data with their published papers.

News and Views

  • News & Views |

    The crystal structure of a prokaryotic proton-driven fumarate transporter, the first for the diverse SLC26 transporter family, reveals a rare transmembrane-segment topology. The opposite orientation of two short central helices leads to the formation of a dipole-mediated anion-binding site, which is made alternately accessible to either side of the membrane through the rocking movement of the core and gate domains of the transporter.

    • Reinhart A F Reithmeier
    •  & Trevor F Moraes
  • News & Views |

    The cellular 'alarmone' molecule ZTP accumulates when a critical cellular metabolic pathway is starved for substrate. The high-resolution structure of ZTP bound to its RNA-based sensor reveals unexpected strategies used by RNA to specifically recognize small-molecule ligands within the complex cellular mixture.

    • Marisa D Ruehle
    •  & Jeffrey S Kieft

Articles

  • Article |

    New data show that Clock–Bmal1, the central transcriptional activator that drives expression of circadian target genes, also recruits the Ddb1–Cullin-4 ubiquitin ligase to clock promoters to enhance the subsequent binding of the feedback repressors that generate the circadian periodicity of gene expression.

    • Alfred G Tamayo
    • , Hao A Duong
    • , Maria S Robles
    • , Matthias Mann
    •  & Charles J Weitz
  • Article |

    Crystallographic and cryo-EM analyses using short synthetic single-stranded RNAs unveil the structural basis for recognition of bacterial 23S rRNA and vesicular stomatitis virus by Toll-like receptor 13, which triggers an immune response.

    • Wen Song
    • , Jia Wang
    • , Zhifu Han
    • , Yifan Zhang
    • , Heqiao Zhang
    • , Weiguang Wang
    • , Junbiao Chang
    • , Bingshu Xia
    • , Shilong Fan
    • , Dekai Zhang
    • , Jiawei Wang
    • , Hong-Wei Wang
    •  & Jijie Chai
  • Article |

    Foot-and-mouth disease virus (FMDV) capsids are often unstable, thus limiting their use as vaccines. A computational method was used to strengthen protein-protein interfaces and engineer stabilized FMDV capsids, which generated improved antibody responses in vaccinated calves and guinea pigs.

    • Abhay Kotecha
    • , Julian Seago
    • , Katherine Scott
    • , Alison Burman
    • , Silvia Loureiro
    • , Jingshan Ren
    • , Claudine Porta
    • , Helen M Ginn
    • , Terry Jackson
    • , Eva Perez-Martin
    • , C Alistair Siebert
    • , Guntram Paul
    • , Juha T Huiskonen
    • , Ian M Jones
    • , Robert M Esnouf
    • , Elizabeth E Fry
    • , Francois F Maree
    • , Bryan Charleston
    •  & David I Stuart
  • Article |

    Solution NMR and functional analyses reveal the 3D structure of a transmembrane reductase, the archeal CcdA, and suggest a mechanism for how these enzymes relay electrons across cell membranes.

    • Jessica A Williamson
    • , Seung-Hyun Cho
    • , Jiqing Ye
    • , Jean-Francois Collet
    • , Jonathan R Beckwith
    •  & James J Chou
  • Article |

    Concentrations of cations and ATP affect the binding activities of synaptotagmin-1, to trigger synaptic vesicle exocytosis. In physiological ionic conditions, synaptotagmin-1's interaction with SNARE proteins is prevented, and its binding is confined to PIP2-containing membrane.

    • Yongsoo Park
    • , Jong Bae Seo
    • , Alicia Fraind
    • , Angel Pérez-Lara
    • , Halenur Yavuz
    • , Kyungreem Han
    • , Seung-Ryoung Jung
    • , Iman Kattan
    • , Peter Jomo Walla
    • , MooYoung Choi
    • , David S Cafiso
    • , Duk-Su Koh
    •  & Reinhard Jahn
  • Article |

    In somatic cells, L1 retrotransposition is kept in check by DNA methylation. However, in hypomethylated cells, such as cancer cells or iPSCs, miR-128 represses new retrotransposition events and thus might aid in maintaining genome stability.

    • Matthias Hamdorf
    • , Adam Idica
    • , Dimitrios G Zisoulis
    • , Lindsay Gamelin
    • , Charles Martin
    • , Katie J Sanders
    •  & Irene M Pedersen